A3LUB9 (SSN3_PICST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase SSN3
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cyclin-dependent kinase 8

Gene names

Name:	SSN3
Synonyms:	CDK8
ORF Names:	PICST_89025

Organism

Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis) [Complete proteome]

Taxonomic identifier

322104 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Scheffersomyces › Scheffersomyces stipitis

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the srb8-11 complex. The srb8-11 complex is a regulatory module of the Mediator complex which is itself dependent transcription. The srb8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The srb8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Component of the srb8-11 complex, a regulatory module of the Mediator complex By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Activator Kinase Repressor Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Serine/threonine-protein kinase SSN3		PRO_0000312950
Regions
	Domain	27 – 355	329	Protein kinase
	Nucleotide binding	33 – 41	9	ATP By similarity
	Compositional bias	407 – 424	18	Asn-rich
Sites
	Active site	174	1	Proton acceptor By similarity
	Binding site	59	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3LUB9 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 2A3FA1E2D2D696CA	FASTA	431	49,251
	10 20 30 40 50 60 MASNSILTLG PFKHRKDLTR ESVLSTYQII GYIAAGTYGK VYKAKLKNTA SAEQLFAIKK 70 80 90 100 110 120 FKSDNHSSNR SHHNHDINGN EIVHYTGISQ SAIREMSLCR ELNNKNITKL VDIILENKSI 130 140 150 160 170 180 YMIFEFCEHD LLQIIHYHSH PEVKPIPQAT VKSLIWQILN GVTFLHQNWI FHRDLKPANI 190 200 210 220 230 240 MVSSSGVVKI GDLGLARKFN NPLQSLYTGD KVVVTIWYRA PELLLGARHY TPAIDLWAVG 250 260 270 280 290 300 CILAELLSLR PIFKGEEAKI DINNKKSVPF QKNQFQKIVE VLGTPSMKNW PALNKYPEFI 310 320 330 340 350 360 SFQQQLTTNY PPNLVNWYKM IGSSNKQCLE LLKGLLEYDP LVRLTADNAL VHPYFLELPK 370 380 390 400 410 420 VQENAFEGLN LKYPKRRIYT DDNDIISNQA INQNFKRHGG AYDDQHNNSN NNTNNSLNAN 430 NANNVPRKRA R

References

[1]

"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000498 Genomic DNA. Translation: ABN66561.2.
RefSeq	XP_001384590.2. XM_001384553.1.
3D structure databases
ProteinModelPortal	A3LUB9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4838657.
KEGG	pic:PICST_89025.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
KO	K02208.
OMA	FHSHPEK.
OrthoDB	EOG470XRW.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SSN3_PICST

Accession

Primary (citable) accession number: A3LUB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 24, 2007
Last modified:	May 1, 2013
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program