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A3LUB9 (SSN3_PICST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase SSN3

EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cyclin-dependent kinase 8
Gene names
Name:SSN3
Synonyms:CDK8
ORF Names:PICST_89025
OrganismScheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis) [Complete proteome]
Taxonomic identifier322104 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeScheffersomyces

Protein attributes

Sequence length431 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the srb8-11 complex. The srb8-11 complex is a regulatory module of the Mediator complex which is itself dependent transcription. The srb8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The srb8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactor

Magnesium By similarity.

Subunit structure

Component of the srb8-11 complex, a regulatory module of the Mediator complex By similarity.

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 431431Serine/threonine-protein kinase SSN3
PRO_0000312950

Regions

Domain27 – 355329Protein kinase
Nucleotide binding33 – 419ATP By similarity
Compositional bias407 – 42418Asn-rich

Sites

Active site1741Proton acceptor By similarity
Binding site591ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A3LUB9 [UniParc].

Last modified July 24, 2007. Version 2.
Checksum: 2A3FA1E2D2D696CA

FASTA43149,251
        10         20         30         40         50         60 
MASNSILTLG PFKHRKDLTR ESVLSTYQII GYIAAGTYGK VYKAKLKNTA SAEQLFAIKK 

        70         80         90        100        110        120 
FKSDNHSSNR SHHNHDINGN EIVHYTGISQ SAIREMSLCR ELNNKNITKL VDIILENKSI 

       130        140        150        160        170        180 
YMIFEFCEHD LLQIIHYHSH PEVKPIPQAT VKSLIWQILN GVTFLHQNWI FHRDLKPANI 

       190        200        210        220        230        240 
MVSSSGVVKI GDLGLARKFN NPLQSLYTGD KVVVTIWYRA PELLLGARHY TPAIDLWAVG 

       250        260        270        280        290        300 
CILAELLSLR PIFKGEEAKI DINNKKSVPF QKNQFQKIVE VLGTPSMKNW PALNKYPEFI 

       310        320        330        340        350        360 
SFQQQLTTNY PPNLVNWYKM IGSSNKQCLE LLKGLLEYDP LVRLTADNAL VHPYFLELPK 

       370        380        390        400        410        420 
VQENAFEGLN LKYPKRRIYT DDNDIISNQA INQNFKRHGG AYDDQHNNSN NNTNNSLNAN 

       430 
NANNVPRKRA R 

« Hide

References

[1]"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis."
Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M.
Nat. Biotechnol. 25:319-326(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000498 Genomic DNA. Translation: ABN66561.2.
RefSeqXP_001384590.2. XM_001384553.1.

3D structure databases

ProteinModelPortalA3LUB9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4924.PICST_89025.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4838657.
KEGGpic:PICST_89025.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
KOK02208.
OMAVSQDDND.
OrthoDBEOG7K3TWD.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSSN3_PICST
AccessionPrimary (citable) accession number: A3LUB9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 24, 2007
Last modified: April 16, 2014
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families