A3LTB7 (ALG3_PICST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
EC=2.4.1.258

Alternative name(s):
Asparagine-linked glycosylation protein 6
Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase

Gene names

Name:	ALG3
ORF Names:	PICST_58095

Organism

Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis) [Complete proteome]

Taxonomic identifier

322104 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Scheffersomyces

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc(2)-PP-Dol By similarity.
Catalytic activity	Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the ALG3 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase		PRO_0000350931
Regions
	Topological domain	1 – 48	48	Lumenal Potential
	Transmembrane	49 – 69	21	Helical; Potential
	Topological domain	70 – 131	62	Cytoplasmic Potential
	Transmembrane	132 – 152	21	Helical; Potential
	Topological domain	153 – 172	20	Lumenal Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Topological domain	194 – 203	10	Cytoplasmic Potential
	Transmembrane	204 – 224	21	Helical; Potential
	Topological domain	225	1	Lumenal Potential
	Transmembrane	226 – 243	18	Helical; Potential
	Topological domain	244 – 247	4	Cytoplasmic Potential
	Transmembrane	248 – 270	23	Helical; Potential
	Topological domain	271 – 316	46	Lumenal Potential
	Transmembrane	317 – 337	21	Helical; Potential
	Topological domain	338 – 371	34	Cytoplasmic Potential
	Transmembrane	372 – 392	21	Helical; Potential
	Topological domain	393 – 406	14	Lumenal Potential
	Transmembrane	407 – 427	21	Helical; Potential
	Topological domain	428 – 433	6	Cytoplasmic Potential
	Transmembrane	434 – 454	21	Helical; Potential
	Topological domain	455 – 477	23	Lumenal Potential

Sequences

	Sequence		Length	Mass (Da)
	A3LTB7 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 54DA89F304148739	FASTA	477	53,790
	10 20 30 40 50 60 MSEPESSVAN NDGESTQAPQ LAELTLKNVL GDIVNGVHFI IFDPLGNRFV VPVIILLTSI 70 80 90 100 110 120 ITKVIITKVP YTEIDFVTYM QQIQLVNQGE IDYAEISGDT GPIVYPAGFV QIYQWLYSQT 130 140 150 160 170 180 NGGADIATGQ SIFGYLMTAT VVFVCVAYTM SPTTKPWVLF LLLGSKRLYS IYVLRLFNDC 190 200 210 220 230 240 FTTAAMVGVT VFLQQGSYWY STSSFISFLF AIVGADLFSI AISIKMNALL YLPAVVIIAY 250 260 270 280 290 300 FLVGENILLF AIVLAIVPLV QILVGWKFLL PLFDDEAASQ IRWNYINNAF NFGRKFLFKW 310 320 330 340 350 360 TVNWRFIGEE TFLSDKFSIL LMGGHIVVLL FFIFTRFLNS KVTGKSIWQL IKDAFKPSST 370 380 390 400 410 420 ISSNNKLIDY NVGPKLILLI LATTNVIGVL FSRSLHYQFL SWYCWQLPFL LHSTGWNFIV 430 440 450 460 470 CLVLWGAHEW TWNVYPSTVA SSLVLVGILS SVLVGTWRNE AVWFEENNVV DDEKKNE

References

[1]

"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed: 17334359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000498 Genomic DNA. Translation: ABN66377.2.
RefSeq	XP_001384406.2. XM_001384369.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3LTB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4838968.
GenomeReviews	Gene locus ALG3 in contig CP000498_GR.
KEGG	pic:PICST_58095.
Phylogenomic databases
eggNOG	fuNOG07998.
HOGENOM	HBG525447.
OMA	IHRVAYT.
OrthoDB	EOG4MPN04.
Family and domain databases
InterPro	IPR007873. Glycosyltransferase_ALG3. [Graphical view]
KO	K03845.
PANTHER	PTHR12646. ALG3. 1 hit.
Pfam	PF05208. ALG3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG3_PICST

Accession

Primary (citable) accession number: A3LTB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	July 24, 2007
Last modified:	November 16, 2011
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program