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Reviewed, UniProtKB/Swiss-Prot A3LTB7 (ALG3_PICST)

Last modified November 3, 2009. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
    EC=2.4.1.130
Alternative name(s):
    Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
    Asparagine-linked glycosylation protein 6
Gene names
Name: ALG3
ORF Names: PICST_58095
OrganismPichia stipitis (Yeast) [Complete proteome]
Taxonomic identifier4924 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

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Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc(2)-PP-Dol By similarity.

Catalytic activity

Transfers an alpha-D-mannosyl residue from dolichyl-phosphate D-mannose into membrane lipid-linked oligosaccharide.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the ALG3 family.

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Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
   DomainTransmembrane
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentendoplasmic reticulum

Inferred from electronic annotation. Source: UniProtKB-KW

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
PRO_0000350931

Regions

Topological domain1 – 4848Lumenal Potential
Transmembrane49 – 6921 Potential
Topological domain70 – 13162Cytoplasmic Potential
Transmembrane132 – 15221 Potential
Topological domain153 – 17220Lumenal Potential
Transmembrane173 – 19321 Potential
Topological domain194 – 20310Cytoplasmic Potential
Transmembrane204 – 22421 Potential
Topological domain2251Lumenal Potential
Transmembrane226 – 24318 Potential
Topological domain244 – 2474Cytoplasmic Potential
Transmembrane248 – 27023 Potential
Topological domain271 – 31646Lumenal Potential
Transmembrane317 – 33721 Potential
Topological domain338 – 37134Cytoplasmic Potential
Transmembrane372 – 39221 Potential
Topological domain393 – 40614Lumenal Potential
Transmembrane407 – 42721 Potential
Topological domain428 – 4336Cytoplasmic Potential
Transmembrane434 – 45421 Potential
Topological domain455 – 47723Lumenal Potential

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Sequences

Sequence LengthMass (Da)Tools
A3LTB7-1 [UniParc].

Last modified July 24, 2007. Version 2.
Checksum: 54DA89F304148739

FASTA47753,790
        10         20         30         40         50         60 
MSEPESSVAN NDGESTQAPQ LAELTLKNVL GDIVNGVHFI IFDPLGNRFV VPVIILLTSI 

        70         80         90        100        110        120 
ITKVIITKVP YTEIDFVTYM QQIQLVNQGE IDYAEISGDT GPIVYPAGFV QIYQWLYSQT 

       130        140        150        160        170        180 
NGGADIATGQ SIFGYLMTAT VVFVCVAYTM SPTTKPWVLF LLLGSKRLYS IYVLRLFNDC 

       190        200        210        220        230        240 
FTTAAMVGVT VFLQQGSYWY STSSFISFLF AIVGADLFSI AISIKMNALL YLPAVVIIAY 

       250        260        270        280        290        300 
FLVGENILLF AIVLAIVPLV QILVGWKFLL PLFDDEAASQ IRWNYINNAF NFGRKFLFKW 

       310        320        330        340        350        360 
TVNWRFIGEE TFLSDKFSIL LMGGHIVVLL FFIFTRFLNS KVTGKSIWQL IKDAFKPSST 

       370        380        390        400        410        420 
ISSNNKLIDY NVGPKLILLI LATTNVIGVL FSRSLHYQFL SWYCWQLPFL LHSTGWNFIV 

       430        440        450        460        470 
CLVLWGAHEW TWNVYPSTVA SSLVLVGILS SVLVGTWRNE AVWFEENNVV DDEKKNE

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References

[1]"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis."
Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M.
Nat. Biotechnol. 25:319-326(2007) [PubMed: 17334359] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.

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Cross-references

Sequence databases

CP000498 Genomic DNA. Translation: ABN66377.2.
RefSeqXP_001384406.2.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGA3LTB7.

Genome annotation databases

GeneID4838968.
GenomeReviewsGene locus ALG3 in contig CP000498_GR.
KEGGpic:PICST_58095.

Phylogenomic databases

OMALRLFNDC.

Family and domain databases

InterProIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERPTHR12646. ALG3. 1 hit.
PfamPF05208. ALG3. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameALG3_PICST
AccessionPrimary (citable) accession number: A3LTB7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: July 24, 2007
Last modified: November 3, 2009
This is version 20 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents