A3LTB7 (ALG3_PICST) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 37.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase EC=2.4.1.258 Alternative name(s): Asparagine-linked glycosylation protein 6 Dol-P-Man-dependent alpha(1-3)-mannosyltransferase Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase | ||||
| Gene names |
| ||||
| Organism | Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis) [Complete proteome] | ||||
| Taxonomic identifier | 322104 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Scheffersomyces |
Protein attributes
| Sequence length | 477 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc(2)-PP-Dol By similarity. |
| Catalytic activity | Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. |
| Sequence similarities | Belongs to the ALG3 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane Transmembrane helix |
| Molecular function | Glycosyltransferase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 477 | 477 | Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase | PRO_0000350931 | |||||
Regions | |||||||||
| Topological domain | 1 – 48 | 48 | Lumenal Potential | ||||||
| Transmembrane | 49 – 69 | 21 | Helical; Potential | ||||||
| Topological domain | 70 – 131 | 62 | Cytoplasmic Potential | ||||||
| Transmembrane | 132 – 152 | 21 | Helical; Potential | ||||||
| Topological domain | 153 – 172 | 20 | Lumenal Potential | ||||||
| Transmembrane | 173 – 193 | 21 | Helical; Potential | ||||||
| Topological domain | 194 – 203 | 10 | Cytoplasmic Potential | ||||||
| Transmembrane | 204 – 224 | 21 | Helical; Potential | ||||||
| Topological domain | 225 | 1 | Lumenal Potential | ||||||
| Transmembrane | 226 – 243 | 18 | Helical; Potential | ||||||
| Topological domain | 244 – 247 | 4 | Cytoplasmic Potential | ||||||
| Transmembrane | 248 – 270 | 23 | Helical; Potential | ||||||
| Topological domain | 271 – 316 | 46 | Lumenal Potential | ||||||
| Transmembrane | 317 – 337 | 21 | Helical; Potential | ||||||
| Topological domain | 338 – 371 | 34 | Cytoplasmic Potential | ||||||
| Transmembrane | 372 – 392 | 21 | Helical; Potential | ||||||
| Topological domain | 393 – 406 | 14 | Lumenal Potential | ||||||
| Transmembrane | 407 – 427 | 21 | Helical; Potential | ||||||
| Topological domain | 428 – 433 | 6 | Cytoplasmic Potential | ||||||
| Transmembrane | 434 – 454 | 21 | Helical; Potential | ||||||
| Topological domain | 455 – 477 | 23 | Lumenal Potential | ||||||
Sequences
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References
| [1] | "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed: 17334359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CP000498 Genomic DNA. Translation: ABN66377.2. |
| RefSeq | XP_001384406.2. XM_001384369.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | A3LTB7. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 4838968. |
| GenomeReviews | Gene locus ALG3 in contig CP000498_GR. |
| KEGG | pic:PICST_58095. |
Phylogenomic databases | |
| eggNOG | fuNOG07998. |
| HOGENOM | HBG525447. |
| OMA | IHRVAYT. |
| OrthoDB | EOG4MPN04. |
Family and domain databases | |
| InterPro | IPR007873. Glycosyltransferase_ALG3. [Graphical view] |
| KO | K03845. |
| PANTHER | PTHR12646. ALG3. 1 hit. |
| Pfam | PF05208. ALG3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ALG3_PICST | ||||||||
| Accession | Primary (citable) accession number: A3LTB7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with