Reviewed, UniProtKB/Swiss-Prot A3LT66 (MCR1_PICST)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 2
    EC=1.6.2.2
Alternative name(s):
    Mitochondrial cytochrome b reductase

Gene names

Name:	MCR1
ORF Names:	PICST_44816

Organism

Pichia stipitis (Yeast) [Complete proteome]

Taxonomic identifier

4924 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May mediate the reduction of outer membrane cytochrome b5 By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	NADH-cytochrome b5 reductase 2		PRO_0000330189
Regions
	Transmembrane	15 – 38	24	Potential
	Domain	49 – 153	105	FAD-binding FR-type
	Nucleotide binding	156 – 191	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3LT66-1 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 2CD1C2AB00F2FD44	FASTA	298	32,581
	10 20 30 40 50 60 MSFSRSFSRL ASSKFVLPVA AAAVGLASYS FTSSSFIANE PSKAFKGGDE WIDLKLISSH 70 80 90 100 110 120 DLSHDTKHLV FELPNKDDVS GLVTASLLMT KFVTPKGSNV IRPYTPVSDT EQAGTIDFVV 130 140 150 160 170 180 KKYEGGKMSS HIHDLKPNDT LSFKGPFVKW KWEPNQFKSI ALIGGGTGIT PLYQLIHEIT 190 200 210 220 230 240 KNPADKTQVS LFYGSQTPDD ILIKKELDAL AAKHKDQVKI VYFVDKADAS WKGETGYISK 250 260 270 280 290 EFLQKNLPAP GPDNKIFVCG PPPLYKAVSG PKVSPTDQGE LTGSLAELGF SKENVFKF

References

[1]

"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed: 17334359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000498 Genomic DNA. Translation: ABN66017.2.
RefSeq	XP_001384046.2.
3D structure databases
SMR	A3LT66. Positions 49-298.
ModBase	Search...
Protein-protein interaction databases
STRING	A3LT66.
Genome annotation databases
GeneID	4838868.
GenomeReviews	Gene locus MCR1 in contig CP000498_GR.
KEGG	pic:PICST_44816.
Phylogenomic databases
eggNOG	fuNOG07850.
HOGENOM	HBG591994.
OMA	PVASCIT.
OrthoDB	EOG9TB5TC.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD_bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCR1_PICST

Accession

Primary (citable) accession number: A3LT66

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	July 24, 2007
Last modified:	January 19, 2010
This is version 29 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)