A3LS77 (TRMB_PICST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	TRM8
ORF Names:	PICST_56862

Organism

Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis) [Complete proteome]

Taxonomic identifier

322104 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Scheffersomyces › Scheffersomyces stipitis

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subunit structure	Forms a complex with TRM82 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370603
Regions
	Region	141 – 142	2	S-adenosyl-L-methionine binding By similarity
	Region	177 – 178	2	S-adenosyl-L-methionine binding By similarity
	Region	275 – 277	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	200	1	By similarity
	Binding site	118	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	197	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3LS77 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 62FC5E9AE2A03094	FASTA	303	35,818
	10 20 30 40 50 60 MPKPHQVQVI KDRETQLREQ QEAESKRRTY RDVKEETRKK HKKAHFEASP TPEESTSDKI 70 80 90 100 110 120 SLPRKRYYRQ RAHSNPFSDH RLEYPRSPDD MNWEKLFPGF YDEETKQMTS SVEIADVGCG 130 140 150 160 170 180 YGGLLTKLAP EFPNSLILGM EIRVQVTQYV EDRIIALRNK HEDEDVNFQN IAVLRGNAMK 190 200 210 220 230 240 FLPNFFRKGQ LSKMFFCFPD PHFKQRKHKA RIITNTLLSE YAYVLKEGGV VYTITDVEDL 250 260 270 280 290 300 HNWMVKHLEE HPLFERLSKE WEEQDTCVSI MFNSTEEGQK VTRNKGSKWI ACYRRLPNPE ECV

References

[1]

"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000497 Genomic DNA. Translation: ABN65855.1.
RefSeq	XP_001383884.1. XM_001383847.1.
3D structure databases
ProteinModelPortal	A3LS77.
SMR	A3LS77. Positions 75-299.
ModBase	Search...
Protein-protein interaction databases
STRING	4924.PICST_56862.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4837797.
KEGG	pic:PICST_56862.
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000260965.
KO	K03439.
OMA	LPIRTRP.
OrthoDB	EOG4M3DJF.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_PICST

Accession

Primary (citable) accession number: A3LS77

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	April 3, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program