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A3LS77

- TRMB_PICST

UniProt

A3LS77 - TRMB_PICST

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei197 – 1971S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei200 – 2001UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:TRM8UniRule annotation
ORF Names:PICST_56862
OrganismiScheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
Taxonomic identifieri322104 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeScheffersomyces
ProteomesiUP000002258: Chromosome 3

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303tRNA (guanine-N(7)-)-methyltransferasePRO_0000370603Add
BLAST

Interactioni

Subunit structurei

Forms a complex with TRM82.UniRule annotation

Protein-protein interaction databases

STRINGi4924.PICST_56862.

Structurei

3D structure databases

ProteinModelPortaliA3LS77.
SMRiA3LS77. Positions 75-299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni141 – 1422S-adenosyl-L-methionine bindingUniRule annotation
Regioni177 – 1782S-adenosyl-L-methionine bindingUniRule annotation
Regioni275 – 2773S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000260965.
InParanoidiA3LS77.
KOiK03439.
OMAiHEDESNA.
OrthoDBiEOG708W9T.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3LS77-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPKPHQVQVI KDRETQLREQ QEAESKRRTY RDVKEETRKK HKKAHFEASP
60 70 80 90 100
TPEESTSDKI SLPRKRYYRQ RAHSNPFSDH RLEYPRSPDD MNWEKLFPGF
110 120 130 140 150
YDEETKQMTS SVEIADVGCG YGGLLTKLAP EFPNSLILGM EIRVQVTQYV
160 170 180 190 200
EDRIIALRNK HEDEDVNFQN IAVLRGNAMK FLPNFFRKGQ LSKMFFCFPD
210 220 230 240 250
PHFKQRKHKA RIITNTLLSE YAYVLKEGGV VYTITDVEDL HNWMVKHLEE
260 270 280 290 300
HPLFERLSKE WEEQDTCVSI MFNSTEEGQK VTRNKGSKWI ACYRRLPNPE

ECV
Length:303
Mass (Da):35,818
Last modified:April 3, 2007 - v1
Checksum:i62FC5E9AE2A03094
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000497 Genomic DNA. Translation: ABN65855.1.
RefSeqiXP_001383884.1. XM_001383847.1.

Genome annotation databases

GeneIDi4837797.
KEGGipic:PICST_56862.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000497 Genomic DNA. Translation: ABN65855.1 .
RefSeqi XP_001383884.1. XM_001383847.1.

3D structure databases

ProteinModelPortali A3LS77.
SMRi A3LS77. Positions 75-299.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 4924.PICST_56862.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4837797.
KEGGi pic:PICST_56862.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000260965.
InParanoidi A3LS77.
KOi K03439.
OMAi HEDESNA.
OrthoDBi EOG708W9T.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545.

Entry informationi

Entry nameiTRMB_PICST
AccessioniPrimary (citable) accession number: A3LS77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: April 3, 2007
Last modified: October 29, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3