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A3LS77

- TRMB_PICST

UniProt

A3LS77 - TRMB_PICST

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
TRM8, PICST_56862
Organism
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei197 – 1971S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei200 – 2001 By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    Transfer RNA methyltransferase 8
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:TRM8
    ORF Names:PICST_56862
    OrganismiScheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
    Taxonomic identifieri322104 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeScheffersomyces
    ProteomesiUP000002258: Chromosome 3

    Subcellular locationi

    Nucleus By similarity UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 303303tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_0000370603Add
    BLAST

    Interactioni

    Subunit structurei

    Forms a complex with TRM82 By similarity.UniRule annotation

    Protein-protein interaction databases

    STRINGi4924.PICST_56862.

    Structurei

    3D structure databases

    ProteinModelPortaliA3LS77.
    SMRiA3LS77. Positions 75-299.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni141 – 1422S-adenosyl-L-methionine binding By similarity
    Regioni177 – 1782S-adenosyl-L-methionine binding By similarity
    Regioni275 – 2773S-adenosyl-L-methionine binding By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000260965.
    KOiK03439.
    OMAiHEDESNA.
    OrthoDBiEOG708W9T.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A3LS77-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPKPHQVQVI KDRETQLREQ QEAESKRRTY RDVKEETRKK HKKAHFEASP    50
    TPEESTSDKI SLPRKRYYRQ RAHSNPFSDH RLEYPRSPDD MNWEKLFPGF 100
    YDEETKQMTS SVEIADVGCG YGGLLTKLAP EFPNSLILGM EIRVQVTQYV 150
    EDRIIALRNK HEDEDVNFQN IAVLRGNAMK FLPNFFRKGQ LSKMFFCFPD 200
    PHFKQRKHKA RIITNTLLSE YAYVLKEGGV VYTITDVEDL HNWMVKHLEE 250
    HPLFERLSKE WEEQDTCVSI MFNSTEEGQK VTRNKGSKWI ACYRRLPNPE 300
    ECV 303
    Length:303
    Mass (Da):35,818
    Last modified:April 3, 2007 - v1
    Checksum:i62FC5E9AE2A03094
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000497 Genomic DNA. Translation: ABN65855.1.
    RefSeqiXP_001383884.1. XM_001383847.1.

    Genome annotation databases

    GeneIDi4837797.
    KEGGipic:PICST_56862.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000497 Genomic DNA. Translation: ABN65855.1 .
    RefSeqi XP_001383884.1. XM_001383847.1.

    3D structure databases

    ProteinModelPortali A3LS77.
    SMRi A3LS77. Positions 75-299.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 4924.PICST_56862.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4837797.
    KEGGi pic:PICST_56862.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000260965.
    KOi K03439.
    OMAi HEDESNA.
    OrthoDBi EOG708W9T.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545.

    Entry informationi

    Entry nameiTRMB_PICST
    AccessioniPrimary (citable) accession number: A3LS77
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: April 3, 2007
    Last modified: June 11, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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