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A3LS77 (TRMB_PICST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:TRM8
ORF Names:PICST_56862
OrganismScheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis) [Complete proteome]
Taxonomic identifier322104 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeScheffersomyces

Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with TRM82 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 303303tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370603

Regions

Region141 – 1422S-adenosyl-L-methionine binding By similarity
Region177 – 1782S-adenosyl-L-methionine binding By similarity
Region275 – 2773S-adenosyl-L-methionine binding By similarity

Sites

Active site2001 By similarity
Binding site1181S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1971S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
A3LS77 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: 62FC5E9AE2A03094

FASTA30335,818
        10         20         30         40         50         60 
MPKPHQVQVI KDRETQLREQ QEAESKRRTY RDVKEETRKK HKKAHFEASP TPEESTSDKI 

        70         80         90        100        110        120 
SLPRKRYYRQ RAHSNPFSDH RLEYPRSPDD MNWEKLFPGF YDEETKQMTS SVEIADVGCG 

       130        140        150        160        170        180 
YGGLLTKLAP EFPNSLILGM EIRVQVTQYV EDRIIALRNK HEDEDVNFQN IAVLRGNAMK 

       190        200        210        220        230        240 
FLPNFFRKGQ LSKMFFCFPD PHFKQRKHKA RIITNTLLSE YAYVLKEGGV VYTITDVEDL 

       250        260        270        280        290        300 
HNWMVKHLEE HPLFERLSKE WEEQDTCVSI MFNSTEEGQK VTRNKGSKWI ACYRRLPNPE 


ECV 

« Hide

References

[1]"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis."
Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M.
Nat. Biotechnol. 25:319-326(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000497 Genomic DNA. Translation: ABN65855.1.
RefSeqXP_001383884.1. XM_001383847.1.

3D structure databases

ProteinModelPortalA3LS77.
SMRA3LS77. Positions 75-299.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4924.PICST_56862.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4837797.
KEGGpic:PICST_56862.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000260965.
KOK03439.
OMACENDICV.
OrthoDBEOG708W9T.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_PICST
AccessionPrimary (citable) accession number: A3LS77
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: April 3, 2007
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways