Reviewed, UniProtKB/Swiss-Prot A3LRX6 (LKA41_PICST)

Names and origin

Protein names

Recommended name:
    Leukotriene A-4 hydrolase 1
    EC=3.3.2.6
Alternative name(s):
    Leukotriene A(4) hydrolase
      Short name=LTA-4 hydrolase

Gene names

Name:	LKA4
ORF Names:	PICST_76777

Organism

Pichia stipitis (Yeast) [Complete proteome]

Taxonomic identifier

4924 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	626 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes an epoxide moiety of LTA4 to form LTB4. The enzyme also has some peptidase activity By similarity.
Catalytic activity	(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O = (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Lipid metabolism; leukotriene B4 biosynthesis.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	leukotriene-A4 hydrolase activity Inferred from electronic annotation. Source: EC metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 626	626	Leukotriene A-4 hydrolase 1		PRO_0000324938
Sites
	Active site	289	1	By similarity
	Active site	389	1	Proton donor By similarity
	Metal binding	288	1	Zinc; catalytic By similarity
	Metal binding	292	1	Zinc; catalytic By similarity
	Metal binding	311	1	Zinc; catalytic By similarity
	Site	192	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3LRX6-1 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: FD6212CA990E2688	FASTA	626	71,543
	10 20 30 40 50 60 MSKFENISKR SHELDPCTNS NYAEFKVVHT DLSLSVSFES KNISGNVIYT LKKLTKTNRL 70 80 90 100 110 120 VLDTSFLEVS TAKVNGKEVV FELLPHKAPF GSPLVIPIDG ENESLTVEVD FRTTDKCTAI 130 140 150 160 170 180 QFIQGDTGPY VFSQCQAIHA RSLFPCFDTP GVKSPYKFTA KSPSVCTMSG RPQPSNEAGV 190 200 210 220 230 240 YHFDQPIPIP SYLVSITSGN LHKAPIGPRS DVYSEEPSLK DCQWEFEKDM ENFIQIAEKI 250 260 270 280 290 300 VFEYEWSRFD SLVLPSSFPY GGMEIPNMTQ LTPTLISKDR TQVKVMAHEL AHSWSGNLVT 310 320 330 340 350 360 NCSWEHFWLN EGWTVYLERR IIGAIAAAEA QEEGRANPEK YGEQVRHFNA IIGWNALVET 370 380 390 400 410 420 VESFDPKFTS LVWDLASGDP DDAFSRIPYE KGFNFLFHIE TKVGGTKEFD PFIKHYFKKF 430 440 450 460 470 480 RYQSLNSAQF IETLYDFYTP LGKKDALDTI DLEKWLFQPG LPDDPKFDTT LADQVYVLVE 490 500 510 520 530 540 KWVDFVKSGE TEVKFSEADV KEFEGEQEML FIETLTDRFK DLDVSPELIR KLPSIYPKYA 550 560 570 580 590 600 ASKNGEVLAR WNELLIKYGN YTSSDEQVKF FADWLGTVGR MKYVRPGYKL LQTSVSIEFA 610 620 VETFKRFEDK YHPICKTMVQ KDLNLA

References

[1]

"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed: 17334359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000497 Genomic DNA. Translation: ABN65808.2.
RefSeq	XP_001383837.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3LRX6.
Genome annotation databases
GeneID	4837771.
GenomeReviews	Gene locus LKA4 in contig CP000497_GR.
KEGG	pic:PICST_76777.
Phylogenomic databases
OMA	SERCAVW.
Enzyme and pathway databases
BRENDA	3.3.2.6. 81766.
Family and domain databases
InterPro	IPR012777. Leuk_A4_hydro_aminopept. IPR006025. Pept_M_Zn_BS. IPR001930. Peptidase_M1. IPR015211. Peptidase_M1_C. IPR014782. Peptidase_M1_N. [Graphical view]
PANTHER	PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF09127. Leuk-A4-hydro_C. 1 hit. PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
TIGRFAMs	TIGR02411. leuko_A4_hydro. 1 hit.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LKA41_PICST

Accession

Primary (citable) accession number: A3LRX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	July 24, 2007
Last modified:	November 3, 2009
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)