Reviewed, UniProtKB/Swiss-Prot A3LQI7 (LKA42_PICST)

Names and origin

Protein names

Recommended name:
    Leukotriene A-4 hydrolase 2
    EC=3.3.2.6
Alternative name(s):
    Leukotriene A(4) hydrolase
      Short name=LTA-4 hydrolase

Gene names

Name:	LTA4
ORF Names:	PICST_82220

Organism

Pichia stipitis (Yeast) [Complete proteome]

Taxonomic identifier

4924 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	635 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes an epoxide moiety of LTA4 to form LTB4. The enzyme also has some peptidase activity By similarity.
Catalytic activity	(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O = (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Lipid metabolism; leukotriene B4 biosynthesis.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	leukotriene-A4 hydrolase activity Inferred from electronic annotation. Source: EC metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 635	635	Leukotriene A-4 hydrolase 2		PRO_0000324939
Sites
	Active site	295	1	By similarity
	Active site	399	1	Proton donor By similarity
	Metal binding	294	1	Zinc; catalytic By similarity
	Metal binding	298	1	Zinc; catalytic By similarity
	Metal binding	317	1	Zinc; catalytic By similarity
	Site	198	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3LQI7-1 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 71C9ADE733E4C1DA	FASTA	635	73,074
	10 20 30 40 50 60 MNVIKSRRPA VSPEIDPSTL SNYQNFAVSN TELFLDVLFD EKIVAGTVLY HLEVLNKDVS 70 80 90 100 110 120 EVILDTSFLT VESVTVNGSE AEFSLHERVE PLGSRLAISI PPNSQKVQLF ISFRTTERCT 130 140 150 160 170 180 AIQFLDKEAT DGKKAPYLFC QCQAIHARSL FPCFDTPALK STYKLSAKSP LFTLMSGRPV 190 200 210 220 230 240 NQEGDMYYFD QPVPIPSYLI SIASGDIVKA KIGPRSDIYS EPVKIKDCQW EFENDMEDFI 250 260 270 280 290 300 QIAEKLVFEY EWLRFDSLVL PSSFPYGGME IPNLCQLTPT LICKDRSQVT VMAHELAHSW 310 320 330 340 350 360 SGNLVTNCSW EHFWLNEGWT VYIERRIIEG IATAEAIKAG KKDPFAYGES MRHFSAIIGW 370 380 390 400 410 420 NDLENSIKAM GNDAERFSPL VLDLKAGEDP DDSFSTVPYE KGFNLLFLIE QTVGGKKVFD 430 440 450 460 470 480 KFIPSYFKKF RYGSLDTYQF VDYLYEFFND KKVELDSIDW ESWLYKPGMP PVMPKFDTTL 490 500 510 520 530 540 ADECYELADE WFSAIKNNSY LKHDFSSADI KSFEPNQSVV FLDTLISYNK HKDFNWKDHV 550 560 570 580 590 600 DALKLMETAY TEYDTSLNAE ILFRWYMLQV SGEREEFQHK LGQWLGTVGR MKFVRPGYVL 610 620 630 LNEVNRELAI YYFKKFESNY HPICKTMVKK DLGLV

References

[1]

"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed: 17334359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000496 Genomic DNA. Translation: ABN64689.2.
RefSeq	XP_001382718.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4837028.
GenomeReviews	Gene locus LTA4 in contig CP000496_GR.
KEGG	pic:PICST_82220.
Phylogenomic databases
OMA	FTNRIVE.
Enzyme and pathway databases
BRENDA	3.3.2.6. 81766.
Family and domain databases
InterPro	IPR012777. Leuk_A4_hydro_aminopept. IPR006025. Pept_M_Zn_BS. IPR001930. Peptidase_M1. IPR015211. Peptidase_M1_C. IPR014782. Peptidase_M1_N. [Graphical view]
PANTHER	PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF09127. Leuk-A4-hydro_C. 1 hit. PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
TIGRFAMs	TIGR02411. leuko_A4_hydro. 1 hit.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LKA42_PICST

Accession

Primary (citable) accession number: A3LQI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	July 24, 2007
Last modified:	November 3, 2009
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)