Reviewed, UniProtKB/Swiss-Prot A3LQD7 (KYNU_PICST)

Names and origin

Protein names

Recommended name:
    Kynureninase
    EC=3.7.1.3
Alternative name(s):
    L-kynurenine hydrolase
    Biosynthesis of nicotinic acid protein 5

Gene names

Name:	BNA5
ORF Names:	PICST_55542

Organism

Pichia stipitis (Yeast) [Complete proteome]

Taxonomic identifier

4924 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively By similarity.
Catalytic activity	L-kynurenine + H2O = anthranilate + L-alanine. L-3-hydroxykynurenine + H2O = 3-hydroxyanthranilate + L-alanine.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1. Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the kynureninase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro tryptophan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kynureninase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Kynureninase		PRO_0000356984
Regions
	Region	144 – 147	4	Pyridoxal phosphate binding By similarity
Sites
	Binding site	116	1	Pyridoxal phosphate; via amide nitrogen By similarity
	Binding site	117	1	Pyridoxal phosphate By similarity
	Binding site	231	1	Pyridoxal phosphate By similarity
	Binding site	234	1	Pyridoxal phosphate By similarity
	Binding site	256	1	Pyridoxal phosphate By similarity
	Binding site	291	1	Pyridoxal phosphate By similarity
	Binding site	319	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	257	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3LQD7-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: AB373BBF56454822	FASTA	465	52,840
	10 20 30 40 50 60 MSLEKAQELD TKYPTYKDEF AVPTFKSLGI ETSQFNENTD SIYLCGNSLG LMPKSTKQAI 70 80 90 100 110 120 NDELNAWIER GVESHFNHPG QSEGKTPWVD IDLPLVPLVA PIVGAKENEV AVMGSLTSNL 130 140 150 160 170 180 NALLINFYKP KDKRTKILFE KQAFPSDYYA FLNLVKLHGY DESHLVQLKV LPGNTYLTTE 190 200 210 220 230 240 QIKKAVDDNI DELAMVCFPG IQYYTGQFFK IEEITNYVKN KGKNEIVVGW DLAHAVGNVP 250 260 270 280 290 300 LKLHDWNVDF AAWCSYKYLN SGPGGIAGIY VHEQYTKDNS KTSFSPRLAG WWGNNASDRF 310 320 330 340 350 360 KMLEEFDPIN SALSYRQSNP SVIDVVAVKS SLELFKKVGG VPKLRKKSIA LTQFLQDLLV 370 380 390 400 410 420 SSKYYFRSDN DTDRSKIGFT ILTPLNQEER GAQLSVLFQP HYDDKQKNIM ERVSGYLHNH 430 440 450 460 AIICDERRPD VIRFAPLPLY NTFEETYYAA QRLFEALDKI SSEEI

References

[1]

"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed: 17334359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000496 Genomic DNA. Translation: ABN65189.1.
RefSeq	XP_001383218.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3LQD7.
Genome annotation databases
GeneID	4836702.
GenomeReviews	Gene locus BNA5 in contig CP000496_GR.
KEGG	pic:PICST_55542.
Phylogenomic databases
OMA	WQPLSGW.
Family and domain databases
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR010111. Kynureninase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR14084. Kynureninase. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
TIGRFAMs	TIGR01814. kynureninase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KYNU_PICST

Accession

Primary (citable) accession number: A3LQD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)