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A3LQ22

- A3LQ22_PICST

UniProt

A3LQ22 - A3LQ22_PICST

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Protein

Histone-lysine N-methyltransferase, H3 lysine-79 specific

Gene

PICST_41120

Organism
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].UniRule annotation

Enzyme regulationi

Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.UniRule annotation

GO - Molecular functioni

  1. histone-lysine N-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulatorUniRule annotation, MethyltransferaseUniRule annotation, Transferase

Keywords - Ligandi

S-adenosyl-L-methionineUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-79 specificUniRule annotation (EC:2.1.1.43UniRule annotation)
Alternative name(s):
Histone H3-K79 methyltransferaseUniRule annotation
Gene namesi
ORF Names:PICST_41120Imported
OrganismiScheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)Imported
Taxonomic identifieri322104 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeScheffersomyces
ProteomesiUP000002258: Chromosome 2

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

NucleusUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi4924.PICST_41120.

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.UniRule annotation
Contains 1 DOT1 domain.UniRule annotation

Phylogenomic databases

InParanoidiA3LQ22.
KOiK11427.
OMAiDVLIINN.
OrthoDBiEOG7KH9VN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013110. DOT1.
IPR025789. Histone_H3-K79_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51569. DOT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

A3LQ22-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
VKTLTASNVW TQDEIEHFHT CIVHNATRTG MGHLIEQKSI AELNNAVTQL
60 70 80 90 100
MSRFYWTKGE MTFLENHLGA GVAELCHEIP LRAKHGIVQM KSVRDLEVTI
110 120 130 140 150
AEKKVVNLTD SSMEDTTEDD NLAYCIQHDL SRETLEALFP DWSLSETLDR
160 170 180 190 200
IVFQAGPLDS IALTTGEKQI IKDSIKKQKS LESVQFNFPC RSKDYLVEKF
210 220 230 240 250
KEFEFVSSRK TKFKSLSERL LYEAKWVLYS SGETFTTTRR SRKRALEESF
260 270 280 290 300
ETMEKEALVS IYTKPAPKQE MTPEELEERE RRREALKESR RLANERKALL
310 320 330 340 350
RKQRKEDLER RKAAGLIKAK PKSDITHSIK DLLAGSEHFQ SVIGDKKKVE
360 370 380 390 400
EGQKRKRIQA VHYRPEIEPK KPSKLKVRHR QAEKSKIKLA LKLKKQQEHN
410 420 430 440 450
KRKPKKEPKK KKKTVEEESL ATPETEEFIK EEIDEDEEEE EEEDTYSPFD
460 470 480 490 500
PVDLNSDSFV PLHGRQFYAE EIYVDKPHVP ELKFVEVTEE SENSLISSST
510 520 530 540 550
LTETKRIMTT NDDDIVYEDC LAADIIRSHI KNYRDLPISF PPLLDPSSSE
560 570 580 590 600
RKIYPTNKVR IRFLLYPQHC ESFILAAPKT NELDPVYEII KLFMIHYALF
610 620 630 640 650
FSHSSEIRRI ITEEYCQKIE HSIEENDFSD FMFVVDKWNA LMLKLSPNEE
660 670 680 690 700
AVQSIVQSGK EDINAGLRSY LSEQEIRVPT SEDLKLQAFL EAVILEDLSP
710 720 730 740 750
TFQLIKEEPS DAEKQEFVPK HLGDVEAPKN VSDELKDMKP DDYNLIFFTR
760 770 780 790 800
LKEKTEISRF ATQQILLRIY SRIVSTDSRK LRSYKAFTAE VYGELLPSFT
810 820 830 840 850
SEVLEKVNLL PNQKFYDLGS GVGNTTFQAA LEFGASMSGG CELMEHASKL
860 870 880 890 900
TKLQEGLLQK HMAVLGLKKL NFNFALSQSF VDNDPVRDAA SDCEVLIINN
910 920 930 940 950
YLFDGNLNAE VGRLLCGLKP GTKIISLRNF ISPRYRATGD TIFDFFKVEK
960 970 980 990 1000
HEMSDFLSVS WTANKVPYYI STVQDRILPE YLGKDESPDS DRSTPTLKSE
1010 1020 1030
NGSSENLAGS LTPFTATPEP DMFKDLSCVL GDEDDILLH
Length:1,039
Mass (Da):119,382
Last modified:July 24, 2007 - v2
Checksum:i37D9E081E7B24A97
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11Imported

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000496 Genomic DNA. Translation: ABN64600.2.
RefSeqiXP_001382629.2. XM_001382592.1.

Genome annotation databases

GeneIDi4837176.
KEGGipic:PICST_41120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000496 Genomic DNA. Translation: ABN64600.2 .
RefSeqi XP_001382629.2. XM_001382592.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 4924.PICST_41120.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4837176.
KEGGi pic:PICST_41120.

Phylogenomic databases

InParanoidi A3LQ22.
KOi K11427.
OMAi DVLIINN.
OrthoDBi EOG7KH9VN.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR013110. DOT1.
IPR025789. Histone_H3-K79_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF08123. DOT1. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 2 hits.
PROSITEi PS51569. DOT1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545Imported.

Entry informationi

Entry nameiA3LQ22_PICST
AccessioniPrimary (citable) accession number: A3LQ22
Entry historyi
Integrated into UniProtKB/TrEMBL: April 3, 2007
Last sequence update: July 24, 2007
Last modified: October 29, 2014
This is version 46 of the entry and version 2 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3