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A3LQ22 (A3LQ22_PICST) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Histone-lysine N-methyltransferase, H3 lysine-79 specific RuleBase RU271113

EC=2.1.1.43 RuleBase RU271113
Alternative name(s):
Histone H3-K79 methyltransferase RuleBase RU271113
Gene names
ORF Names:PICST_41120 EMBL ABN64600.2
OrganismScheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis) [Complete proteome] EMBL ABN64600.2
Taxonomic identifier322104 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeScheffersomyces

Protein attributes

Sequence length1039 AA.
Sequence statusFragment.
Protein existenceInferred from homology

General annotation (Comments)

Function

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones By similarity. RuleBase RU271113

Catalytic activity

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]. RuleBase RU271113

Enzyme regulation

Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3 By similarity. RuleBase RU271113

Subcellular location

Nucleus By similarity RuleBase RU271113.

Miscellaneous

In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones By similarity. RuleBase RU271113

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family. RuleBase RU271113

Contains 1 DOT1 domain. RuleBase RU271113

Ontologies

Keywords
   Cellular componentNucleus RuleBase RU271113
   LigandS-adenosyl-L-methionine RuleBase RU271113
   Molecular functionChromatin regulator RuleBase RU271113
Methyltransferase RuleBase RU271113
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionhistone-lysine N-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Experimental info

Non-terminal residue11 EMBL ABN64600.2

Sequences

Sequence LengthMass (Da)Tools
A3LQ22 [UniParc].

Last modified July 24, 2007. Version 2.
Checksum: 37D9E081E7B24A97

FASTA1,039119,382
        10         20         30         40         50         60 
VKTLTASNVW TQDEIEHFHT CIVHNATRTG MGHLIEQKSI AELNNAVTQL MSRFYWTKGE 

        70         80         90        100        110        120 
MTFLENHLGA GVAELCHEIP LRAKHGIVQM KSVRDLEVTI AEKKVVNLTD SSMEDTTEDD 

       130        140        150        160        170        180 
NLAYCIQHDL SRETLEALFP DWSLSETLDR IVFQAGPLDS IALTTGEKQI IKDSIKKQKS 

       190        200        210        220        230        240 
LESVQFNFPC RSKDYLVEKF KEFEFVSSRK TKFKSLSERL LYEAKWVLYS SGETFTTTRR 

       250        260        270        280        290        300 
SRKRALEESF ETMEKEALVS IYTKPAPKQE MTPEELEERE RRREALKESR RLANERKALL 

       310        320        330        340        350        360 
RKQRKEDLER RKAAGLIKAK PKSDITHSIK DLLAGSEHFQ SVIGDKKKVE EGQKRKRIQA 

       370        380        390        400        410        420 
VHYRPEIEPK KPSKLKVRHR QAEKSKIKLA LKLKKQQEHN KRKPKKEPKK KKKTVEEESL 

       430        440        450        460        470        480 
ATPETEEFIK EEIDEDEEEE EEEDTYSPFD PVDLNSDSFV PLHGRQFYAE EIYVDKPHVP 

       490        500        510        520        530        540 
ELKFVEVTEE SENSLISSST LTETKRIMTT NDDDIVYEDC LAADIIRSHI KNYRDLPISF 

       550        560        570        580        590        600 
PPLLDPSSSE RKIYPTNKVR IRFLLYPQHC ESFILAAPKT NELDPVYEII KLFMIHYALF 

       610        620        630        640        650        660 
FSHSSEIRRI ITEEYCQKIE HSIEENDFSD FMFVVDKWNA LMLKLSPNEE AVQSIVQSGK 

       670        680        690        700        710        720 
EDINAGLRSY LSEQEIRVPT SEDLKLQAFL EAVILEDLSP TFQLIKEEPS DAEKQEFVPK 

       730        740        750        760        770        780 
HLGDVEAPKN VSDELKDMKP DDYNLIFFTR LKEKTEISRF ATQQILLRIY SRIVSTDSRK 

       790        800        810        820        830        840 
LRSYKAFTAE VYGELLPSFT SEVLEKVNLL PNQKFYDLGS GVGNTTFQAA LEFGASMSGG 

       850        860        870        880        890        900 
CELMEHASKL TKLQEGLLQK HMAVLGLKKL NFNFALSQSF VDNDPVRDAA SDCEVLIINN 

       910        920        930        940        950        960 
YLFDGNLNAE VGRLLCGLKP GTKIISLRNF ISPRYRATGD TIFDFFKVEK HEMSDFLSVS 

       970        980        990       1000       1010       1020 
WTANKVPYYI STVQDRILPE YLGKDESPDS DRSTPTLKSE NGSSENLAGS LTPFTATPEP 

      1030 
DMFKDLSCVL GDEDDILLH 

« Hide

References

[1]"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis."
Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M.
Nat. Biotechnol. 25:319-326(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000496 Genomic DNA. Translation: ABN64600.2.
RefSeqXP_001382629.2. XM_001382592.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4924.PICST_41120.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4837176.
KEGGpic:PICST_41120.

Phylogenomic databases

KOK11427.
OMADVLIINN.
OrthoDBEOG7KH9VN.

Family and domain databases

InterProIPR013110. DOT1.
IPR025789. Histone_H3-K79_MeTrfase.
[Graphical view]
PfamPF08123. DOT1. 1 hit.
[Graphical view]
PROSITEPS51569. DOT1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA3LQ22_PICST
AccessionPrimary (citable) accession number: A3LQ22
Entry history
Integrated into UniProtKB/TrEMBL: April 3, 2007
Last sequence update: July 24, 2007
Last modified: April 16, 2014
This is version 42 of the entry and version 2 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)