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A3LQ22

- A3LQ22_PICST

UniProt

A3LQ22 - A3LQ22_PICST

Protein

Histone-lysine N-methyltransferase, H3 lysine-79 specific

Gene

PICST_41120

Organism
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 2 (24 Jul 2007)
      Previous versions | rss
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    Functioni

    Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].UniRule annotation

    Enzyme regulationi

    Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.UniRule annotation

    GO - Molecular functioni

    1. histone-lysine N-methyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Chromatin regulatorUniRule annotation, MethyltransferaseUniRule annotation, Transferase

    Keywords - Ligandi

    S-adenosyl-L-methionineUniRule annotation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histone-lysine N-methyltransferase, H3 lysine-79 specificUniRule annotation (EC:2.1.1.43UniRule annotation)
    Alternative name(s):
    Histone H3-K79 methyltransferaseUniRule annotation
    Gene namesi
    ORF Names:PICST_41120Imported
    OrganismiScheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)Imported
    Taxonomic identifieri322104 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeScheffersomyces
    ProteomesiUP000002258: Chromosome 2

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    NucleusUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi4924.PICST_41120.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.UniRule annotation
    Contains 1 DOT1 domain.UniRule annotation

    Phylogenomic databases

    KOiK11427.
    OMAiDVLIINN.
    OrthoDBiEOG7KH9VN.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR013110. DOT1.
    IPR025789. Histone_H3-K79_MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF08123. DOT1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 2 hits.
    PROSITEiPS51569. DOT1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    A3LQ22-1 [UniParc]FASTAAdd to Basket

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    VKTLTASNVW TQDEIEHFHT CIVHNATRTG MGHLIEQKSI AELNNAVTQL     50
    MSRFYWTKGE MTFLENHLGA GVAELCHEIP LRAKHGIVQM KSVRDLEVTI 100
    AEKKVVNLTD SSMEDTTEDD NLAYCIQHDL SRETLEALFP DWSLSETLDR 150
    IVFQAGPLDS IALTTGEKQI IKDSIKKQKS LESVQFNFPC RSKDYLVEKF 200
    KEFEFVSSRK TKFKSLSERL LYEAKWVLYS SGETFTTTRR SRKRALEESF 250
    ETMEKEALVS IYTKPAPKQE MTPEELEERE RRREALKESR RLANERKALL 300
    RKQRKEDLER RKAAGLIKAK PKSDITHSIK DLLAGSEHFQ SVIGDKKKVE 350
    EGQKRKRIQA VHYRPEIEPK KPSKLKVRHR QAEKSKIKLA LKLKKQQEHN 400
    KRKPKKEPKK KKKTVEEESL ATPETEEFIK EEIDEDEEEE EEEDTYSPFD 450
    PVDLNSDSFV PLHGRQFYAE EIYVDKPHVP ELKFVEVTEE SENSLISSST 500
    LTETKRIMTT NDDDIVYEDC LAADIIRSHI KNYRDLPISF PPLLDPSSSE 550
    RKIYPTNKVR IRFLLYPQHC ESFILAAPKT NELDPVYEII KLFMIHYALF 600
    FSHSSEIRRI ITEEYCQKIE HSIEENDFSD FMFVVDKWNA LMLKLSPNEE 650
    AVQSIVQSGK EDINAGLRSY LSEQEIRVPT SEDLKLQAFL EAVILEDLSP 700
    TFQLIKEEPS DAEKQEFVPK HLGDVEAPKN VSDELKDMKP DDYNLIFFTR 750
    LKEKTEISRF ATQQILLRIY SRIVSTDSRK LRSYKAFTAE VYGELLPSFT 800
    SEVLEKVNLL PNQKFYDLGS GVGNTTFQAA LEFGASMSGG CELMEHASKL 850
    TKLQEGLLQK HMAVLGLKKL NFNFALSQSF VDNDPVRDAA SDCEVLIINN 900
    YLFDGNLNAE VGRLLCGLKP GTKIISLRNF ISPRYRATGD TIFDFFKVEK 950
    HEMSDFLSVS WTANKVPYYI STVQDRILPE YLGKDESPDS DRSTPTLKSE 1000
    NGSSENLAGS LTPFTATPEP DMFKDLSCVL GDEDDILLH 1039
    Length:1,039
    Mass (Da):119,382
    Last modified:July 24, 2007 - v2
    Checksum:i37D9E081E7B24A97
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11Imported

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000496 Genomic DNA. Translation: ABN64600.2.
    RefSeqiXP_001382629.2. XM_001382592.1.

    Genome annotation databases

    GeneIDi4837176.
    KEGGipic:PICST_41120.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000496 Genomic DNA. Translation: ABN64600.2 .
    RefSeqi XP_001382629.2. XM_001382592.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 4924.PICST_41120.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4837176.
    KEGGi pic:PICST_41120.

    Phylogenomic databases

    KOi K11427.
    OMAi DVLIINN.
    OrthoDBi EOG7KH9VN.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR013110. DOT1.
    IPR025789. Histone_H3-K79_MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF08123. DOT1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 2 hits.
    PROSITEi PS51569. DOT1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545Imported.

    Entry informationi

    Entry nameiA3LQ22_PICST
    AccessioniPrimary (citable) accession number: A3LQ22
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 3, 2007
    Last sequence update: July 24, 2007
    Last modified: October 1, 2014
    This is version 45 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3