Reviewed, UniProtKB/Swiss-Prot A3LNF8 (KMO_PICST)

Names and origin

Protein names

Recommended name:
    Kynurenine 3-monooxygenase
    EC=1.14.13.9
Alternative name(s):
    Kynurenine 3-hydroxylase
    Biosynthesis of nicotinic acid protein 4

Gene names

Name:	BNA4
ORF Names:	PICST_40545

Organism

Pichia stipitis (Yeast) [Complete proteome]

Taxonomic identifier

4924 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid By similarity.
Catalytic activity	L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the aromatic-ring hydroxylase family. KMO subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Mitochondrion
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro kynurenine 3-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Kynurenine 3-monooxygenase		PRO_0000361932

Sequences

	Sequence		Length	Mass (Da)
	A3LNF8-1 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 3F983C198687AEAE	FASTA	478	54,389
	10 20 30 40 50 60 MSVLESVDTS NRHQGVGIVG AGLVGCLAAL AFAAKGYSVT LFELRPDPKT VDASERNLRS 70 80 90 100 110 120 INLAVSNRGI RALKYVDDAM ADRILEHIIP MKGRMIHDTT GTKQESQLYG LFGESINSID 130 140 150 160 170 180 RGFLNDCLLA EMRHSDDINV LFNHKLVQLD HLMREDETPT MTFVDTRDNK AEPKTFEFDY 190 200 210 220 230 240 IVGADGAHSQ FRYQMQRSMR MDFQQKYIDM QYLELYIPPN TLEGATSKFS IDPNHLHIWP 250 260 270 280 290 300 RHNFMLIALA NKDGSFTSTF FSPWSVIESI KSAQEWVVFF KKNFPDAYKL MGDDHLISVY 310 320 330 340 350 360 ESNPRGTLMQ VTAYPYHNPT GRAIIIGDAA HSMVPFYGQG MNCGFEDVRV LMELIDTNHG 370 380 390 400 410 420 NVTKSFKQYS DARKKDLDAI CKLALDNYHE MSSKVTSPLY LIRKKLDYTL GKYANGTLFQ 430 440 450 460 470 WLPLYTMISF RDDIPYAKAI AIEKRQATIL NRVQIVSLTA LALYGAVKAA QCWDRFRR

References

[1]

"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed: 17334359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000496 Genomic DNA. Translation: ABN64850.2.
RefSeq	XP_001382879.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3LNF8.
Genome annotation databases
GeneID	4836628.
GenomeReviews	Gene locus BNA4 in contig CP000496_GR.
KEGG	pic:PICST_40545.
Phylogenomic databases
OMA	LHAIMPS.
Family and domain databases
InterPro	IPR002218. GIDA-rel. IPR003042. Rng_hydrolase-like. [Graphical view]
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

KMO_PICST

Accession

Primary (citable) accession number: A3LNF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	July 24, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)