Reviewed, UniProtKB/Swiss-Prot A3LN91 (HOG1_PICST)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase HOG1
      Short name=MAP kinase HOG1
    EC=2.7.11.24

Gene names

Name:	HOG1
ORF Names:	PICST_54431

Organism

Pichia stipitis (Yeast) [Complete proteome]

Taxonomic identifier

4924 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-174 and Tyr-176, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Mitogen-activated protein kinase HOG1		PRO_0000289700
Regions
	Domain	23 – 305	283	Protein kinase
	Nucleotide binding	29 – 37	9	ATP By similarity
	Motif	174 – 176	3	TXY By similarity
Sites
	Active site	144	1	Proton acceptor By similarity
	Binding site	52	1	ATP By similarity
Amino acid modifications
	Modified residue	174	1	Phosphothreonine By similarity
	Modified residue	176	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3LN91-1 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 7F3EB9BB7AD80556	FASTA	385	44,024
	10 20 30 40 50 60 MSSDGEFTRT QIFGTVFEIT NRYTDLNPVG MGAFGLVCSA VDKLTGQNVA VKKIMKPFST 70 80 90 100 110 120 SVLAKRTYRE LKLLKHLKHE NLITLDDIFL SPLEDIYFVN ELQGTDLHRL LTSRPLEKQF 130 140 150 160 170 180 IQYFTYQILR GLKYIHSAGV IHRDLKPSNI LINENCDLKI CDFGLARIQD PQMTGYVSTR 190 200 210 220 230 240 YYRAPEIMLT WQKYDTEVDL WSVGCILAEM IEGKPLFPGK DHVHQFSIIT ELLGSPPPDV 250 260 270 280 290 300 IDTICSENTL RFVQSLPHRD PIPFNERFAQ CTHVEPEAID LLAKMLIFDP KKRISAASAL 310 320 330 340 350 360 THPYMEPYHD PTDEPICETK FDWSFNDADL PVDTWRVMMY SEILDFHQIG GVGEEAGQSV 370 380 TQEEIAHIQQ DGIQAPQQPQ EQQVE

References

[1]

"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed: 17334359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000496 Genomic DNA. Translation: ABN64819.2.
RefSeq	XP_001382848.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4837018.
GenomeReviews	Gene locus HOG1 in contig CP000496_GR.
KEGG	pic:PICST_54431.
Phylogenomic databases
OMA	SEILDFH.
Enzyme and pathway databases
BRENDA	2.7.11.24. 81766.
Family and domain databases
InterPro	IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOG1_PICST

Accession

Primary (citable) accession number: A3LN91

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 24, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)