A3KPF5 (PDI15_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein disulfide isomerase-like 1-5
Short name=AtPDIL1-5
EC=5.3.4.1

Alternative name(s):
Protein disulfide isomerase 3
Short name=AtPDI3
Protein disulfide isomerase-like 3-1
Short name=AtPDIL3-1

Gene names

Name:	PDIL1-5
Synonyms:	PDI3, PDIL3-1
Ordered Locus Names:	At1g52260
ORF Names:	F19K6.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds By similarity.
Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen Probable.
Tissue specificity	Widely expressed. Ref.5
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.
Sequence caution	The sequence AAG51554.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 16618929PubMed 22923678. Source: TAIR endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein disulfide isomerase activity Inferred from sequence or structural similarity Ref.5. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 537	508	Protein disulfide isomerase-like 1-5		PRO_0000400020
Regions
	Domain	58 – 184	127	Thioredoxin 1
	Domain	380 – 526	147	Thioredoxin 2
	Motif	534 – 537	4	Prevents secretion from ER By similarity
Sites
	Active site	106	1	Nucleophile By similarity
	Active site	447	1	Nucleophile By similarity
	Active site	450	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	160	1	N-linked (GlcNAc...) Potential
	Glycosylation	364	1	N-linked (GlcNAc...) Potential
	Glycosylation	416	1	N-linked (GlcNAc...) Potential
	Glycosylation	530	1	N-linked (GlcNAc...) Potential
	Disulfide bond	447 ↔ 450		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3KPF5 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 6380842BE6CFB1C4	FASTA	537	60,158
	10 20 30 40 50 60 MSLIPKPISK VSTFTFILLI LLSFTIIIAY SSPDSNVESN EPGFDSDLDQ LLAVDEQLQE 70 80 90 100 110 120 DRPEQQSEAE TVSKAQRIVL ELNGDYTKRV IDGNEFVMVL GYAPWCARSA ELMPRFAEAA 130 140 150 160 170 180 TALKEIGSSV LMAKIDGDRY SKIASELEIK GFPTLLLFVN GTSLTYNGGS SAEDIVIWVQ 190 200 210 220 230 240 KKTGAPIITL NTVDEAPRFL DKYHTFVLGL FEKFEGSEHN EFVKAAKSDD EIQFIETRDS 250 260 270 280 290 300 DVAKLLFPDL KSNNVFIGLV KPEAERYTVY DGSYKMEKIL EFLGSNKFPL FTKLTETNTV 310 320 330 340 350 360 WVYSSPVKLQ VMLFSKADDF QKLAQPLEDI ARKFKSKLMF IYVDITNENL AMPFLILFGI 370 380 390 400 410 420 EAGNKTVVAA FDNNLNSKYL LESDPSPNSI EEFCSGLAHG TVSRYYRSEP VPDNENASIV 430 440 450 460 470 480 TVVGKTFDGL VLNSRENVLL EVHTPWCVNC EALSKQIEKL AKHFKGFENL VFARIDASAN 490 500 510 520 530 EHTKLQVDDK YPIILLYKSG EKEKPLKLST KLSAKDIAVF INEELLKPKN GSAKDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins." Houston N.L., Fan C., Xiang J.Q., Schulze J.M., Jung R., Boston R.S. Plant Physiol. 137:762-778(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[5]	"Endoplasmic reticulum stress activates the expression of a sub-group of protein disulfide isomerase genes and AtbZIP60 modulates the response in Arabidopsis thaliana." Lu D.-P., Christopher D.A. Mol. Genet. Genomics 280:199-210(2008) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"The protein disulfide isomerase gene family in bread wheat (T. aestivum L.)." d'Aloisio E., Paolacci A.R., Dhanapal A.P., Tanzarella O.A., Porceddu E., Ciaffi M. BMC Plant Biol. 10:101-101(2010) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC037424 Genomic DNA. Translation: AAG51554.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE32775.1. BT030322 mRNA. Translation: ABO09885.1.
IPI	IPI00520170.
PIR	F96562.
RefSeq	NP_175636.2. NM_104105.2.
UniGene	At.49972.
3D structure databases
ProteinModelPortal	A3KPF5.
SMR	A3KPF5. Positions 79-528.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G52260.1-P.
Proteomic databases
PaxDb	A3KPF5.
PRIDE	A3KPF5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G52260.1; AT1G52260.1; AT1G52260.
GeneID	841656.
KEGG	ath:AT1G52260.
Organism-specific databases
TAIR	At1g52260.
Phylogenomic databases
eggNOG	COG0526.
HOGENOM	HOG000241706.
InParanoid	A3KPF5.
KO	K09580.
OMA	SANEHTK.
PhylomeDB	A3KPF5.
ProtClustDB	CLSN2680753.
Gene expression databases
Genevestigator	A3KPF5.
Family and domain databases
Gene3D	3.40.30.10. 3 hits.
InterPro	IPR012336. Thioredoxin-like_fold. IPR013766. Thioredoxin_domain. [Graphical view]
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 4 hits.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. False negative. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDI15_ARATH

Accession

Primary (citable) accession number: A3KPF5
Secondary accession number(s): Q9C818

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2010
Last sequence update:	April 3, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program