Reviewed, UniProtKB/Swiss-Prot A3KPF2 (CARMB_ARATH)

Names and origin

Protein names

Recommended name:
    Probable histone-arginine methyltransferase CARM1B
    EC=2.1.1.125
    EC=2.1.1.-
Alternative name(s):
    Protein arginine N-methyltransferase 4B

Gene names

Name:	CARM1B
Synonyms:	PRMT4B
Ordered Locus Names:	At5g49020
ORF Names:	K19E20.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation, methylates histone H3 and activates transcription via chromatin remodeling By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone-arginine = S-adenosyl-L-homocysteine + histone-N(omega)-methyl-arginine.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity.
Sequence similarities	Belongs to the protein arginine N-methyltransferase family.
Sequence caution	The sequence BAB10326.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histone H3-R17 methylation Inferred from genetic interaction. Source: TAIR histone H3-R2 methylation Inferred from direct assay. Source: TAIR histone H3-R26 methylation Inferred from direct assay. Source: TAIR peptidyl-arginine methylation, to asymmetrical-dimethyl arginine Inferred from direct assay. Source: TAIR regulation of flower development Inferred from genetic interaction. Source: TAIR regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW vegetative to reproductive phase transition Inferred from genetic interaction. Source: TAIR
Cellular component	cytoplasm Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay. Source: TAIR
Molecular function	[myelin basic protein]-arginine N-methyltransferase activity Inferred from direct assay. Source: TAIR histone-arginine N-methyltransferase activity Inferred from direct assay. Source: TAIR protein heterodimerization activity Inferred from physical interaction. Source: TAIR protein homodimerization activity Inferred from physical interaction. Source: TAIR protein-arginine omega-N asymmetric methyltransferase activity Inferred from direct assay. Source: TAIR protein-arginine omega-N monomethyltransferase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A3KPF2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A3KPF2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     123-124: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 528	528	Probable histone-arginine methyltransferase CARM1B		PRO_0000294005
Natural variations
	Alternative sequence	123 – 124	2	Missing in isoform 2.		VSP_026577

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 31BC878D35445BD8	FASTA	528	58,857
	10 20 30 40 50 60 MEIPSLNKQQ EFTLASVTDL TSPSSSLSSS PVVATFSCVN EVKELRFQES KSSDGFSFDL 70 80 90 100 110 120 SSTQLFKLGP LQFTCVSDGS ISSAKEKSSF SRGVVIKFRD EKDSKEFCDS FEECKKDDAV 130 140 150 160 170 180 KQGSALPNGT VVSANKSKFD DKIEAASAKM YFHYYGQLLH QQNMLQDYVR TGTYHAAVME 190 200 210 220 230 240 NRSDFSGRVV VDVGAGSGIL SMFAALAGAK HVYAVEASEM AEYARKLIAG NPLLAERITV 250 260 270 280 290 300 IKGKIEDIEL PEKADVLISE PMGTLLVNER MLETYVIARD RFLSPNGKMF PTVGRIHMAP 310 320 330 340 350 360 FADEFLFVEM ANKALFWQQQ NYYGVDLTPL YVSAHQGYFS QPVVDAFDPR LLVAPSMFHV 370 380 390 400 410 420 IDFTMMTEEQ FYEIDIPLKF TASVCTRIHG LACWFDVLFD GSTVQRWFTT APGAPTTHWY 430 440 450 460 470 480 QIRCVLSQPI HVMAGQEITG RLHLIAHSAQ SYTINLTLSA KMWGPGANQG GILQTSSCKL 490 500 510 520 DLKEPYYRMS QPQVYPTQEP PAQSQDIHIH SDDLEELELL QQNANAQL
	Isoform 2. Checksum: 0EFB2A5FC319DDA8	FASTA	526	58,713
	10 20 30 40 50 60 MEIPSLNKQQ EFTLASVTDL TSPSSSLSSS PVVATFSCVN EVKELRFQES KSSDGFSFDL 70 80 90 100 110 120 SSTQLFKLGP LQFTCVSDGS ISSAKEKSSF SRGVVIKFRD EKDSKEFCDS FEECKKDDAV 130 140 150 160 170 180 KQALPNGTVV SANKSKFDDK IEAASAKMYF HYYGQLLHQQ NMLQDYVRTG TYHAAVMENR 190 200 210 220 230 240 SDFSGRVVVD VGAGSGILSM FAALAGAKHV YAVEASEMAE YARKLIAGNP LLAERITVIK 250 260 270 280 290 300 GKIEDIELPE KADVLISEPM GTLLVNERML ETYVIARDRF LSPNGKMFPT VGRIHMAPFA 310 320 330 340 350 360 DEFLFVEMAN KALFWQQQNY YGVDLTPLYV SAHQGYFSQP VVDAFDPRLL VAPSMFHVID 370 380 390 400 410 420 FTMMTEEQFY EIDIPLKFTA SVCTRIHGLA CWFDVLFDGS TVQRWFTTAP GAPTTHWYQI 430 440 450 460 470 480 RCVLSQPIHV MAGQEITGRL HLIAHSAQSY TINLTLSAKM WGPGANQGGI LQTSSCKLDL 490 500 510 520 KEPYYRMSQP QVYPTQEPPA QSQDIHIHSD DLEELELLQQ NANAQL

References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S. DNA Res. 6:183-195(1999) [PubMed: 10470850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Arabidopsis ORF clones." Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 94-528. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB017061 Genomic DNA. Translation: BAB10326.1. Sequence problems. BT030319 mRNA. Translation: ABO09883.1. BT030323 mRNA. Translation: ABO09886.1. BT002972 mRNA. Translation: AAO22781.1.
IPI	IPI00523648. IPI00544269.
RefSeq	NP_199713.2. NP_974913.1.
UniGene	At.27882
3D structure databases
HSSP	HSSP built from PDB template 1G6Q based on UniProtKB P38074.
ModBase	Search...
Proteomic databases
PRIDE	A3KPF2.
Genome annotation databases
GeneID	834961.
GenomeReviews	Gene locus AT5G49020 in contig BA000015_GR.
KEGG	ath:AT5G49020.
NMPDR	fig|3702.1.peg.26740.
Organism-specific databases
TAIR	At5g49020.
Phylogenomic databases
OMA	LFWQQQN.
Enzyme and pathway databases
BRENDA	2.1.1.125. 302.
Gene expression databases
Genevestigator	A3KPF2.
Family and domain databases
InterPro	IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARMB_ARATH

Accession

Primary (citable) accession number: A3KPF2
Secondary accession number(s): Q3E8E0, Q84WN5, Q9FI68

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)