A3KPA3 (NAA60_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-alpha-acetyltransferase 60
EC=2.3.1.48
EC=2.3.1.88

Alternative name(s):
N-acetyltransferase 15
NatF catalytic subunit

Gene names

Name:	naa60
Synonyms:	nat15
ORF Names:	zgc:163109

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Histone acetyltransferase localized in the Golgi apparatus that mediates acetylation of free histone H4, thereby facilitating nucleosome assembly. Has a preference for free histone H4 'Lys-20'(H4K20ac), 'Lys-79'(H4K79ac) and 'Lys-91' (H4K91ac). Also displays alpha (N-terminal) acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase By similarity.
Catalytic activity	Acetyl-CoA + peptide = N(alpha)-acetylpeptide + CoA. Acetyl-CoA + [histone] = CoA + acetyl-[histone].
Subcellular location	Golgi apparatus membrane By similarity.
Sequence similarities	Belongs to the acetyltransferase family. NAA60 subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Chromosome partition
Cellular component	Golgi apparatus Membrane
Molecular function	Acyltransferase Chromatin regulator Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-terminal peptidyl-methionine acetylation Inferred from sequence or structural similarity. Source: UniProtKB cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB chromosome segregation Inferred from sequence or structural similarity. Source: UniProtKB nucleosome assembly Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	Golgi membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	H4 histone acetyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB peptide alpha-N-acetyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	N-alpha-acetyltransferase 60		PRO_0000321569
Regions
	Domain	13 – 182	170	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	A3KPA3 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 8FC6B77734949A09	FASTA	242	27,601
	10 20 30 40 50 60 MTDVVPTTAL SEIQLRLLCH DDIDRIKVLC GEWFPIEYPD SWYHDITSNK KFFSLAATFR 70 80 90 100 110 120 GGIVGMIVAE IKSRTKVHKE DGDILASSFP VDTQVAYILS LGVVKEFRKH GIGSLLLDSL 130 140 150 160 170 180 KEHISTTAQD HCKAIYLHVL TTNNTAIHFY ENRDFKQHHY LPYYYSIRGV LKDGFTYVLY 190 200 210 220 230 240 INGGHPPWTI FDYIHHIGSA LASLSPCSIP QRIYRQAQNL LRSFLPWSGI SSKSGIEYSR TM

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC134236 mRNA. Translation: AAI34237.1.
IPI	IPI00512433.
RefSeq	NP_001076341.1. NM_001082872.1.
UniGene	Dr.159507.
3D structure databases
ProteinModelPortal	A3KPA3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000086994; ENSDARP00000081428; ENSDARG00000061185.
GeneID	570640.
KEGG	dre:570640.
Organism-specific databases
CTD	570640.
ZFIN	ZDB-GENE-041111-143. nat15.
Phylogenomic databases
eggNOG	NOG276305.
GeneTree	ENSGT00390000008314.
HOGENOM	HOG000280717.
HOVERGEN	HBG061680.
InParanoid	A3KPA3.
OMA	PWTILYP.
OrthoDB	EOG45TCNW.
Gene expression databases
Bgee	A3KPA3.
Family and domain databases
Gene3D	3.40.630.30. 1 hit.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GNAT_dom. [Graphical view]
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20890227.

Entry information

Entry name

NAA60_DANRE

Accession

Primary (citable) accession number: A3KPA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	April 3, 2007
Last modified:	April 3, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program