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A3KNW0 (PLD6_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Mitochondrial cardiolipin hydrolase
EC=3.1.4.-
Alternative name(s):
Choline phosphatase 6
Mitochondrial phospholipase
Short name=MitoPLD
Phosphatidylcholine-hydrolyzing phospholipase D6
Phospholipase D6
Short name=PLD 6
Gene names
Name:pld6
ORF Names:zgc:162591
OrganismDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cardiolipin hydrolase present at the mitochondrial outer membrane required both for mitochondrial fusion and piRNA metabolic process. Acts by catalyzing the hydrolysis of cardiolipin (diphosphatidylglycerol) to form phosphatidate (phosphatidic acid or PA) at the mitochondrial outer membrane surface, promoting mitochondrial fusion. The production of phosphatidate also regulates the piRNA metabolic process by promoting recruitment and/or activation of components of the meiotic nuage, also named P granule, a critical step for primary biogenesis of piRNAs. Required during spermatogenesis to repress transposable elements and prevent their mobilization via its role in the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion outer membrane; Single-pass membrane protein By similarity.

Domain

In contrast to other members of the phospholipase D family, contains only one PLD phosphodiesterase domain, suggesting that it has a single half-catalytic and requires homodimerization to form a complete active site By similarity.

Sequence similarities

Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily.

Contains 1 PLD phosphodiesterase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227Mitochondrial cardiolipin hydrolase
PRO_0000325912

Regions

Topological domain1 – 1414Mitochondrial intermembrane Potential
Transmembrane15 – 3319Helical; Potential
Topological domain34 – 227194Cytoplasmic Potential
Domain153 – 18028PLD phosphodiesterase

Sites

Active site1581 Potential
Active site1601 Potential
Active site1651 Potential

Experimental info

Sequence conflict731F → L in AAI34036. Ref.1
Sequence conflict2161L → H in AAI34036. Ref.1

Sequences

Sequence LengthMass (Da)Tools
A3KNW0 [UniParc].

Last modified May 3, 2011. Version 2.
Checksum: 08A46DAC6C218155

FASTA22725,866
        10         20         30         40         50         60 
MDVFKQMSFK ELMKVLGLGT VAFVLGVEWL NWLTRRLRDS RGPLKEVLFF PSPQVCVEHL 

        70         80         90        100        110        120 
FTSHRSFPCA CPFPHGIQTS FSRLLEHLLS ARTSLEMCIF SFSNMEMSRA ILLLHKRGVV 

       130        140        150        160        170        180 
VRVVTDRDYM TITGSQIGAL RKAGISVRHE MSSAVHMHHK FALVDGRKLI SGSLNWTLTA 

       190        200        210        220 
VQSNKENVII TEEPELVRPF QQEFLKLWEA SDPANLKLQS KNGQIKK

« Hide

References

[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX901942 Genomic DNA. Translation: CAX14562.1.
BC134035 mRNA. Translation: AAI34036.1.
IPIIPI00503736.
RefSeqNP_001082883.1. NM_001089414.1.
UniGeneDr.79030.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000083946; ENSDARP00000078381; ENSDARG00000059951.
GeneID567338.
KEGGdre:567338.

Organism-specific databases

ZFINZDB-GENE-030131-3825. zgc:162591.

Phylogenomic databases

eggNOGfiNOG15850.
HOGENOMHBG714389.
HOVERGENHBG108267.
InParanoidA3KNW0.
OMAPRREVLF.
OrthoDBEOG402WTD.

Gene expression databases

BgeeA3KNW0.

Family and domain databases

PROSITEPS50035. PLD. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePLD6_DANRE
AccessionPrimary (citable) accession number: A3KNW0
Secondary accession number(s): B8A667
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 3, 2011
Last modified: September 21, 2011
This is version 33 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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