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Protein

ATPase inhibitor A, mitochondrial

Gene

atpif1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endogenous F1F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F1F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F1F(o)-ATP synthase enzyme acts as an ATP hydrolase (By similarity). Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing fech to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • erythrocyte differentiation Source: UniProtKB
  • heme biosynthetic process Source: UniProtKB
  • negative regulation of ATPase activity Source: GO_Central
  • negative regulation of hydrolase activity Source: UniProtKB
  • protein homotetramerization Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase inhibitor A, mitochondrial
Alternative name(s):
Inhibitor of F(1)F(o)-ATPase A
Short name:
IF(1) A
Short name:
IF1 A
Protein pinotage
Gene namesi
Name:atpif1
Synonyms:atpia, atpif1a, pnt
ORF Names:Zgc:162207
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 19

Organism-specific databases

ZFINiZDB-GENE-070410-36 atp5if1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Defects in circulating erythroid cells. Embryos are anemic despite normal expression of the erythroid cell markers. The erythrocytes from embryos that survive to adult stage exhibit hypochromic, microcytic anemia. Histological analysis of adult haematopoietic tissues does not show morphological defects. Defects are due to changes in the mitochondrial pH-and consequently the redox potential-to change to a level that reduces [2Fe-2S] cluster-containing fech activity, thereby reducing heme synthesis, resulting in hypochromic anemia.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000421768? – 105ATPase inhibitor A, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

PaxDbiA3KNL5
PeptideAtlasiA3KNL5

Expressioni

Gene expression databases

BgeeiENSDARG00000067975
ExpressionAtlasiA3KNL5 baseline and differential

Interactioni

Subunit structurei

Homodimer; represents the active form and is present at a pH value below 6.5. Homotetramer; represents the inactive form and is present at a pH value above 7.0 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000081676

Structurei

3D structure databases

ProteinModelPortaliA3KNL5
SMRiA3KNL5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 51N-terminal inhibitory regionBy similarityAdd BLAST30
Regioni73 – 105Antiparallel alpha-helical coiled coil regionBy similarityAdd BLAST33

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili58 – 105Sequence analysisAdd BLAST48

Domaini

Forms an alpha-helical dimer with monomers associated via an antiparallel alpha-helical coiled coil composed of residues 73-105, leaving each N-terminal inhibitory region (residues 22-51) accessible for interaction with an F1 catalytic domain. The inhibitory N-terminal region (residues 22-51) binds the alpha(ADP-bound)-beta(ADP-bound) (ATP5F1A-ATP5F1B) interface of F1-ATPase, and also contact the central gamma subunit (ATP5F1C). This dimeric state is favored by pH values below 7.0, and at higher values the dimers associate to form inactive homotetramer, where the inhibitory region is occluded, masking its inhibitory activity (By similarity).By similarity

Sequence similaritiesi

Belongs to the ATPase inhibitor family.Curated

Keywords - Domaini

Coiled coil, Transit peptide

Phylogenomic databases

eggNOGiENOG410J2TJ Eukaryota
ENOG41127Z5 LUCA
GeneTreeiENSGT00390000006264
HOGENOMiHOG000247022
HOVERGENiHBG061381
InParanoidiA3KNL5
KOiK22255
OMAiQEVDHHK
PhylomeDBiA3KNL5
TreeFamiTF320659

Family and domain databases

InterProiView protein in InterPro
IPR007648 ATPase_inhibitor_mt
PfamiView protein in Pfam
PF04568 IATP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A3KNL5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARLLLRRGF FSSHIRMSSD QLGELGTGAG KGGGGGGSVR AAGGSFGRRE
60 70 80 90 100
AAEEERYFRQ KEREQLAALK NHHEEEIDHH KKEIERLQRE IDRHKGKIRK

LKHDD
Length:105
Mass (Da):11,944
Last modified:April 3, 2007 - v1
Checksum:i851D1A097A16239E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU633999 Genomic DNA No translation available.
CU634003 Genomic DNA No translation available.
BC133905 mRNA Translation: AAI33906.1
RefSeqiNP_001082990.1, NM_001089521.1
UniGeneiDr.51212

Genome annotation databases

EnsembliENSDART00000087242; ENSDARP00000081676; ENSDARG00000067975
GeneIDi100037369
KEGGidre:100037369

Similar proteinsi

Entry informationi

Entry nameiATF1A_DANRE
AccessioniPrimary (citable) accession number: A3KNL5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: April 3, 2007
Last modified: May 23, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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