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Protein

Pikachurin

Gene

EGFLAM

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Pikachurin
Alternative name(s):
EGF-like, fibronectin type-III and laminin G-like domain-containing protein
Gene namesi
Name:EGFLAM
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Secretedextracellular spaceextracellular matrix By similarity
  • Cell junctionsynapse By similarity

  • Note: Detected in the synaptic cleft of the ribbon synapse around the postsynaptic terminals of bipolar cells. Colocalizes with BSN, CTBP2 and DAG1 in photoreceptor synaptic terminals.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Extracellular matrix, Secreted, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 1018995PikachurinPRO_0000306802Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence analysis
Disulfide bondi356 ↔ 367By similarity
Disulfide bondi361 ↔ 378By similarity
Disulfide bondi380 ↔ 389By similarity
Disulfide bondi543 ↔ 573By similarity
Disulfide bondi578 ↔ 589By similarity
Disulfide bondi583 ↔ 599By similarity
Disulfide bondi601 ↔ 610By similarity
Disulfide bondi797 ↔ 808By similarity
Disulfide bondi802 ↔ 817By similarity
Disulfide bondi819 ↔ 828By similarity
Disulfide bondi988 ↔ 1015By similarity

Post-translational modificationi

O-glycosylated; contains chondroitin sulfate and heparan sulfate.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiA3KN33.
PRIDEiA3KN33.

Interactioni

Subunit structurei

Interacts with DAG1 alpha-dystroglycan.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026111.

Structurei

3D structure databases

ProteinModelPortaliA3KN33.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 145109Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini153 – 24896Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini352 – 39039EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini395 – 573179Laminin G-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini574 – 61138EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini618 – 797180Laminin G-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini793 – 82937EGF-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini836 – 1015180Laminin G-like 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 EGF-like domains.PROSITE-ProRule annotation
Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 3 laminin G-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0613. Eukaryota.
KOG3509. Eukaryota.
ENOG410XTD2. LUCA.
HOGENOMiHOG000112344.
HOVERGENiHBG107839.
InParanoidiA3KN33.

Family and domain databases

Gene3Di2.60.120.200. 3 hits.
2.60.40.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR001791. Laminin_G.
[Graphical view]
PfamiPF00008. EGF. 2 hits.
PF00041. fn3. 2 hits.
PF00054. Laminin_G_1. 2 hits.
PF02210. Laminin_G_2. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00060. FN3. 2 hits.
SM00282. LamG. 3 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49899. SSF49899. 3 hits.
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 3 hits.
PS50853. FN3. 2 hits.
PS50025. LAM_G_DOMAIN. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A3KN33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLIRGVLLR LLLLASSLGP GAAPLRSALR KQGKVGPPLD IILDALNCSA
60 70 80 90 100
FSIQWKMPRH PPSPIMGYTV FYSEVGIDKS LQERSYGVPP GLDTPTSGRL
110 120 130 140 150
DHQMIFEEVI GDLKPGTEYR VSMAAYSQTG KGRLSSPQHV TTLPQDSCLP
160 170 180 190 200
PTAPQQPHVI VVSDSEVALS WKPGESEGSS PIQYYSVEFT RPDFDKSWTS
210 220 230 240 250
IREQIQMDSM VIKGLDPDTN YQFAVRAVNP HGSSPRSQPS STIRTARPEE
260 270 280 290 300
SGSGRYGPHY ATDTEAGEDD DTFEDDLDLD ISFEEVKPLP AIKEGNKKFF
310 320 330 340 350
VESKMAPRPN PMTVSRLVPP TPASLPTTAV APQPTPVDRK GKHGVAVMPR
360 370 380 390 400
LFDTSCDETV CSADSFCVSD YTWGGSRCHC NLGKGGESCS EDIVIQYPQF
410 420 430 440 450
FGHSYVTFEP LKNSYQAFQI TLEFRAEAED GLLLYCGENE HGRGDFMSLA
460 470 480 490 500
VIRRSLQFRF NCGTGVAIIV SETKIKLGGW HTVTLYRDGL NGLLQLNNGT
510 520 530 540 550
PVTGQSQGQY SKITFRTPLY LGGAPSAYWL VRATGTNRGF QGCVQALTVN
560 570 580 590 600
GKRLDLRPWP LGKALSGADV GECSSGICDE ASCINGGTCM ASKADSYICL
610 620 630 640 650
CPLGFRGRHC EDAFTLTIPQ FKESLRSYAA TPWPLEPRHY LSFMEFEVTF
660 670 680 690 700
RPDSEDGVLL YSYDTGSKDF LSINMAGGHV EFRFDCGSGT GVLRSEEPLT
710 720 730 740 750
LGHWHELCVS RTAKNGILQV DKQKAVEGMA EGGFTQIKCN SDIFIGGVPN
760 770 780 790 800
YDDVKKNSGI LKPFSGSIQK IILNDRTIHV KHDFTWGVNV ENAAHPCVGS
810 820 830 840 850
PCAHGGSCRP RKEGYECDCP LGFEGLHCQK AITEAIEIPQ FIGRSYLTYD
860 870 880 890 900
NPDILKRVSG SRSNAFMRFK TTAKDGLLLW RGDSPMRPNS DFISLGLRDG
910 920 930 940 950
ALVFSYNLGS GVASIMVNGS FNDGRWHRVK AVRDGQSGKI TVDDYGARTG
960 970 980 990 1000
KSPGMMRQLN INGALYVGGM KEIALHTNRQ YMRGLVGCIS HFTLSTDYHI
1010
SLVEDAVDGK NINTCGAK
Length:1,018
Mass (Da):111,405
Last modified:April 3, 2007 - v1
Checksum:i440577855A45D92A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC133537 mRNA. Translation: AAI33538.1.
RefSeqiNP_001076947.1. NM_001083478.1.
UniGeneiBt.12654.

Genome annotation databases

GeneIDi534427.
KEGGibta:534427.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC133537 mRNA. Translation: AAI33538.1.
RefSeqiNP_001076947.1. NM_001083478.1.
UniGeneiBt.12654.

3D structure databases

ProteinModelPortaliA3KN33.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026111.

Proteomic databases

PaxDbiA3KN33.
PRIDEiA3KN33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi534427.
KEGGibta:534427.

Organism-specific databases

CTDi133584.

Phylogenomic databases

eggNOGiKOG0613. Eukaryota.
KOG3509. Eukaryota.
ENOG410XTD2. LUCA.
HOGENOMiHOG000112344.
HOVERGENiHBG107839.
InParanoidiA3KN33.

Family and domain databases

Gene3Di2.60.120.200. 3 hits.
2.60.40.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR001791. Laminin_G.
[Graphical view]
PfamiPF00008. EGF. 2 hits.
PF00041. fn3. 2 hits.
PF00054. Laminin_G_1. 2 hits.
PF02210. Laminin_G_2. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00060. FN3. 2 hits.
SM00282. LamG. 3 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49899. SSF49899. 3 hits.
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 3 hits.
PS50853. FN3. 2 hits.
PS50025. LAM_G_DOMAIN. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEGFLA_BOVIN
AccessioniPrimary (citable) accession number: A3KN33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: April 3, 2007
Last modified: June 8, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.