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Protein

RWD domain-containing protein 3

Gene

RWDD3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the: binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Positively regulates the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Negatively regulates the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Has no effect on ubiquitination (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
RWD domain-containing protein 3
Gene namesi
Name:RWDD3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Colocalizes with UBC9/UBE2I in nuclear spots.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267RWD domain-containing protein 3PRO_0000339462Add
BLAST

Proteomic databases

PaxDbiA3KN24.

Interactioni

Subunit structurei

Interacts with UBE2I/UBC9, NFKBIA, HIF1A and NCOA2.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000028701.

Structurei

3D structure databases

ProteinModelPortaliA3KN24.
SMRiA3KN24. Positions 1-121.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 114108RWDPROSITE-ProRule annotationAdd
BLAST

Domaini

The RWD domain is required for the sumoylation enhancement activity.By similarity

Sequence similaritiesi

Contains 1 RWD domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IGD3. Eukaryota.
ENOG4111KZ9. LUCA.
GeneTreeiENSGT00390000000954.
HOGENOMiHOG000133077.
HOVERGENiHBG057524.
InParanoidiA3KN24.
OMAiQLTRAQC.
OrthoDBiEOG7DZ8KK.
TreeFamiTF324344.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR006575. RWD-domain.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF05773. RWD. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50908. RWD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3KN24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPVRQELS ALAAIFCGPD EWEVLSLSET DGAVFRILTK AEGFMDTDIP
60 70 80 90 100
LQLVFHLPLS YPYCLPGIVV NSKHLTRAQC EIVKEKLLEQ AETLLLEPMV
110 120 130 140 150
HELVLWIQEN LRHILKCPEA GGGSEKCSSA ASMTVDDGLW MTLLHLDHMR
160 170 180 190 200
AKAKYVKTVE KWASDLRLTG RLMFMGKIIL ILLQGDRNDI KEYLILQKTC
210 220 230 240 250
KVDVDSSGKK CKEKMISVLF ETKVQTEHKR FLAFEVKEYS SLDELQKEFE
260
TTGLKKLFSE CVLRLVK
Length:267
Mass (Da):30,406
Last modified:April 3, 2007 - v1
Checksum:i049DDAD1825625B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC133512 mRNA. Translation: AAI33513.1.
RefSeqiNP_001091597.1. NM_001098128.1.
UniGeneiBt.22304.

Genome annotation databases

EnsembliENSBTAT00000028701; ENSBTAP00000028701; ENSBTAG00000021537.
GeneIDi614557.
KEGGibta:614557.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC133512 mRNA. Translation: AAI33513.1.
RefSeqiNP_001091597.1. NM_001098128.1.
UniGeneiBt.22304.

3D structure databases

ProteinModelPortaliA3KN24.
SMRiA3KN24. Positions 1-121.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000028701.

Proteomic databases

PaxDbiA3KN24.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000028701; ENSBTAP00000028701; ENSBTAG00000021537.
GeneIDi614557.
KEGGibta:614557.

Organism-specific databases

CTDi25950.

Phylogenomic databases

eggNOGiENOG410IGD3. Eukaryota.
ENOG4111KZ9. LUCA.
GeneTreeiENSGT00390000000954.
HOGENOMiHOG000133077.
HOVERGENiHBG057524.
InParanoidiA3KN24.
OMAiQLTRAQC.
OrthoDBiEOG7DZ8KK.
TreeFamiTF324344.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR006575. RWD-domain.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF05773. RWD. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50908. RWD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.

Entry informationi

Entry nameiRWDD3_BOVIN
AccessioniPrimary (citable) accession number: A3KN24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: April 3, 2007
Last modified: June 8, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.