A3KGT9 (GPT3L_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase 3-like
EC=2.3.1.15

Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 9-like
Short name=1-AGP acyltransferase 9-like
Short name=1-AGPAT 9-like
Lysophosphatidic acid acyltransferase theta-like
Short name=LPAAT-theta-like

Gene names

Name:	agpat9l
ORF Names:	si:ch211-155m12.4

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis By similarity.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis. Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW triglyceride biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Glycerol-3-phosphate acyltransferase 3-like		PRO_0000291574
Regions
	Transmembrane	15 – 35	21	Helical; Potential
	Transmembrane	146 – 166	21	Helical; Potential
	Transmembrane	170 – 190	21	Helical; Potential
	Transmembrane	358 – 378	21	Helical; Potential
	Motif	238 – 243	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	A3KGT9 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 34F445FFB2C393AD	FASTA	443	50,522
	10 20 30 40 50 60 MEGYWAVLFP VLKVWFSCVI VLIMLPAMFG ISLGITETYM KLLIKTLEWA THRIQRASRA 70 80 90 100 110 120 EEILKESASN GLIQRDNSSL EQEIEELRRN RPKSADRGDF TLSDVLYFSR KGFESIVEDD 130 140 150 160 170 180 VTQRFTSEEL VSWNLLTRTN NNFQYISLRL TVLWVVGVVV RYCILLPLRI TLTTIGLTWL 190 200 210 220 230 240 VIGTTTVGFL PNCRVKNWLS ELVHLMCYRI CARGLSATIH FHNKQNRPKK GGICVANHTS 250 260 270 280 290 300 PIDVVILAND GCYAMVGQVH GGLMGVLQRA MERSCPHIWF ERSEMRDRHL VTQRLKDHVN 310 320 330 340 350 360 AKTKLPILIF PEGTCINNTS VMMFKKGSFE IGGTIYPVAI KYDPQFGDAF WNSSKYSIMS 370 380 390 400 410 420 YLLRMMTSWA IVCNVWYLPP MTHEEGEDAV QFANRVKSTI AQQGGLVDLA WDGGLKRAKV 430 440 KDSFKEQQQK KYSHMVVGED SSD

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL928901 Genomic DNA. Translation: CAM46900.1.
IPI	IPI00512565.
RefSeq	NP_001092920.1. NM_001099450.1.
UniGene	Dr.85777.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3KGT9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000046440; ENSDARP00000046439; ENSDARG00000006491.
GeneID	567414.
KEGG	dre:567414.
Organism-specific databases
CTD	567414.
ZFIN	ZDB-GENE-060531-19. agpat9l.
Phylogenomic databases
eggNOG	fiNOG05701.
InParanoid	A3KGT9.
OMA	KPQKGGI.
OrthoDB	EOG4Z62NH.
Gene expression databases
Bgee	A3KGT9.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
KO	K13506.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPT3L_DANRE

Accession

Primary (citable) accession number: A3KGT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	April 3, 2007
Last modified:	November 16, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program