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Protein

Ral GTPase-activating protein subunit alpha-2

Gene

Ralgapa2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of the heterodimeric RalGAP2 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB.By similarity

GO - Molecular functioni

  • GTPase activator activity Source: MGI
  • protein heterodimerization activity Source: UniProtKB

GO - Biological processi

  • activation of GTPase activity Source: MGI
  • Ral protein signal transduction Source: MGI
  • regulation of exocyst localization Source: MGI
  • regulation of protein localization Source: MGI
  • regulation of small GTPase mediated signal transduction Source: InterPro

Keywordsi

Molecular functionGTPase activation

Names & Taxonomyi

Protein namesi
Recommended name:
Ral GTPase-activating protein subunit alpha-2
Alternative name(s):
250 kDa substrate of Akt
Short name:
AS250
P220
Gene namesi
Name:Ralgapa2
Synonyms:Kiaa1272
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:3036245 Ralgapa2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002869731 – 1872Ral GTPase-activating protein subunit alpha-2Add BLAST1872

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei373PhosphoserineCombined sources1
Modified residuei376PhosphoserineCombined sources1
Modified residuei379PhosphoserineCombined sources1
Modified residuei486PhosphoserineCombined sources1
Modified residuei696Phosphoserine; by PKBBy similarity1
Modified residuei715Phosphothreonine; by PKBBy similarity1
Modified residuei819PhosphoserineCombined sources1
Modified residuei820PhosphoserineCombined sources1
Modified residuei1592PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA3KGS3
PaxDbiA3KGS3
PeptideAtlasiA3KGS3
PRIDEiA3KGS3

PTM databases

iPTMnetiA3KGS3
PhosphoSitePlusiA3KGS3

Expressioni

Tissue specificityi

Abundantly expressed in testis, pancreas, lung, thymus, brown fat, and white fat.1 Publication

Gene expression databases

BgeeiENSMUSG00000037110
CleanExiMM_A230067G21RIK
ExpressionAtlasiA3KGS3 baseline and differential

Interactioni

Subunit structurei

Component of the heterodimeric RalGAP2 complex with RALGAPB. Heterodimerization is required for activity (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi232342, 2 interactors
STRINGi10090.ENSMUSP00000105613

Structurei

3D structure databases

ProteinModelPortaliA3KGS3
SMRiA3KGS3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1634 – 1842Rap-GAPPROSITE-ProRule annotationAdd BLAST209

Phylogenomic databases

eggNOGiKOG3686 Eukaryota
ENOG410XTIX LUCA
GeneTreeiENSGT00760000119182
HOVERGENiHBG051842
InParanoidiA3KGS3
PhylomeDBiA3KGS3
TreeFamiTF324484

Family and domain databases

Gene3Di3.40.50.11210, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR035974 Rap/Ran-GAP_sf
IPR000331 Rap_GAP_dom
IPR027107 Tuberin/Ral-act_asu
PANTHERiPTHR10063 PTHR10063, 1 hit
PfamiView protein in Pfam
PF02145 Rap_GAP, 1 hit
SUPFAMiSSF111347 SSF111347, 1 hit
SSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50085 RAPGAP, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A3KGS3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFSRRSHGDV KKSTQKVLDP KKDVLTRLKH LRALLDNVDA SDLKQFFETN
60 70 80 90 100
YSQIYFIFYE NFITLENSLK LKGNNKSQRE ELDSILFLFE KILQFLPERI
110 120 130 140 150
FFRWHYQSIG STLKKLLHTG NSIKIRCEGI RLFLLWLQAL QTNCAEEQVL
160 170 180 190 200
IFACLVPGFP AVLSSRGPCT LETLINPSPS IVDAKIYPEE ITPLLPAISG
210 220 230 240 250
EKIAEDQTCF FLQILLKYMV IQAASLEWKN KENQDTGFKF LFTLFRKYYL
260 270 280 290 300
PHLFPSFTKL TNIYKPVLEI PHLRPKPVYV TVTRDNETIY STKIPYMAAR
310 320 330 340 350
VVFIKWIVTF FLEKKYLTAT QNTKNGVDVL PKIIQTVGGG AIQEKVPELD
360 370 380 390 400
GAGSTEQDKS HSNSSTLSDR RLSNSSLCSI EEEHRTVYEM VQRILLSTRG
410 420 430 440 450
YVNFVNEVFR QAFLLPSCEI SVTRKVVQVY RKWILQNKPV FMEEPDKKDV
460 470 480 490 500
AQEDADKLGL SETDSKEASS ESSGHKRSSS WGRTYSFTSA MSRGCVTEED
510 520 530 540 550
NTNVKAGAQA MLQVFLTNAA NVFLLEPCAE VPMLLREQVD ASKAVLIIFR
560 570 580 590 600
RMIMELTMNQ KTWEQMLQIL LRITEAVMQK PKDKHVKDLF AQSLAGLLFR
610 620 630 640 650
TLIVAWIRAN LCVYISRELW DDFLRVLSSL TEWEELITEW SNIMDSLTAV
660 670 680 690 700
LARTVYGVEM TNLPLDKLSE QKEKKQRGKG CILEPQKGTA VGRSFSLSWR
710 720 730 740 750
SHPDVTEPMR FRSATTSGAP GVEKARNTVR QKATEVEEFQ QAESTAAADC
760 770 780 790 800
DYLVVGQQQV PRSSSTSDIT ERLYSDSSQG QKVEHSQNLS SSEPKSVQES
810 820 830 840 850
KGHVTHEHEG ITMLVRRSSS PAELELKDDL QQAHGRCRQR QTSESTGSDT
860 870 880 890 900
VVGYSNEAEL PVSPWQACEE DPDLSTPTDA VADSDARHWL QLSPTDASNL
910 920 930 940 950
TDSRECLADD CSIIAGGNLT GWHPDSAAVL WRRVLGILGD VNNIQSPKIH
960 970 980 990 1000
AKVFGYLYEL WYKLAKIRDN LAISLDNQSS PSPPLLIPPL RMFASWLFKA
1010 1020 1030 1040 1050
TTLPNEYKEG KLQAYKLICA MMTRRQDVLP NSDFLVHFYL VMHLGLTSED
1060 1070 1080 1090 1100
QDVLNTIIKN CSPRFFSLGL PGFSMLVGDF ITAAARVLST DMLAAPRSEA
1110 1120 1130 1140 1150
LTLLGSLVCF PNTYQEIPLL QSVPEVSDVV TGAEDVKHYL INILLKNATE
1160 1170 1180 1190 1200
EPNECARCIA ICSLGVWICE ELAQSASHPQ VKDAINVIGV TLKFPNKIVA
1210 1220 1230 1240 1250
QVACDVLQLL VSYWEKLQMF ETALPRKMAE ILVATIAFLL PSAEYSSVET
1260 1270 1280 1290 1300
DKKFIVSLLL CLLDWCMALP VSALLHPVST AVLEELHPSR APLLDYIYRV
1310 1320 1330 1340 1350
LHCCVCGSST YTQQSHYTLT LADLSSTDYD PFLPLANVRN SEPIQYHSSA
1360 1370 1380 1390 1400
DLGNLLTVEE EKKRRSVELI PLTARMVMAH LVNHLGHYPL SGGPAVLHSL
1410 1420 1430 1440 1450
VSENHDNAHV EGTELSSEVF RSPNLQLFVF NDSTLISYLQ TPAEGPAGGT
1460 1470 1480 1490 1500
SGGSLSDVRV IVRDISGKYS WDGKVLYGPL EGRLAPNGRN PSFQISGWHH
1510 1520 1530 1540 1550
HTCGPQKDLF NGEEGDDVLD KLLENIGHTS PECLLPSQLN LNEPSPTPCA
1560 1570 1580 1590 1600
MNWDQEKAIM EVILRQSAQE DEYVQRCNSD SSVTVTSQGQ PSPVEPRGPF
1610 1620 1630 1640 1650
YFCRLLLDDL GMNSWDRRKN FHLLKKNSKL LRELKNLDSR QCRETHKIAV
1660 1670 1680 1690 1700
FYIAEGQEDK CSILANERGS QAYEDFVAGL GWEVDLSTHC GFMGGLQRNG
1710 1720 1730 1740 1750
STGQTAPYYA TSTVEVIFHV STRMPSDSDD SLTKKLRHLG NDEVHIVWSE
1760 1770 1780 1790 1800
HSRDYRRGII PTAFGDVSII IYPMKNHMFF ITITKKPEVP FFGPLFDGAI
1810 1820 1830 1840 1850
VSGKLLPSLI CATCINASRA VKCLIPLYQS FYEERALYLE AIIQNHREVM
1860 1870
TFEDFAAQVF SPSPSYSVSG TD
Length:1,872
Mass (Da):210,288
Last modified:May 15, 2007 - v2
Checksum:iCCB0AA23790D863A
GO
Isoform 2 (identifier: A3KGS3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1789-1807: VPFFGPLFDGAIVSGKLLP → LLLKIEKFHSLGPCSMERS
     1808-1872: Missing.

Show »
Length:1,807
Mass (Da):203,224
Checksum:i69100CE538A321E1
GO

Sequence cautioni

The sequence BAC30146 differs from that shown. Probable cloning artifact leading to erroneous C-terminus.Curated
The sequence BAC30146 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAM46007 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM46125 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM46200 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti436Q → R in BAC30146 (PubMed:19468303).Curated1
Sequence conflicti447K → E in BAE34270 (PubMed:19468303).Curated1
Sequence conflicti1511N → H in AAH53994 (PubMed:15489334).Curated1
Sequence conflicti1727D → V in BAD32418 (PubMed:15368895).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0252511789 – 1807VPFFG…GKLLP → LLLKIEKFHSLGPCSMERS in isoform 2. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_0252521808 – 1872Missing in isoform 2. 1 PublicationAdd BLAST65

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL845273, AL845430, AL935056 Genomic DNA Translation: CAM46125.1 Sequence problems.
AL845273, AL845430, AL935056 Genomic DNA Translation: CAX15311.1
AL845430, AL845273, AL935056 Genomic DNA Translation: CAM46200.1 Sequence problems.
AL845430, AL845273, AL935056 Genomic DNA Translation: CAX15398.1
AL935056, AL845273, AL845430 Genomic DNA Translation: CAM46007.1 Sequence problems.
AL935056, AL845273, AL845430 Genomic DNA Translation: CAX15696.1
AK038838 mRNA Translation: BAC30146.1 Sequence problems.
AK157932 mRNA Translation: BAE34270.1
BC053994 mRNA Translation: AAH53994.1
BC096528 mRNA Translation: AAH96528.1
AK173140 mRNA Translation: BAD32418.1
RefSeqiNP_001028520.2, NM_001033348.3
UniGeneiMm.222310

Genome annotation databases

EnsembliENSMUST00000131824; ENSMUSP00000122039; ENSMUSG00000037110 [A3KGS3-1]
GeneIDi241694
KEGGimmu:241694

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRGPA2_MOUSE
AccessioniPrimary (citable) accession number: A3KGS3
Secondary accession number(s): A3KGS5
, B7ZCU9, Q3TZD9, Q4VA60, Q69ZM8, Q7TQL4, Q8BYP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: March 28, 2018
This is version 92 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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