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Protein

Ral GTPase-activating protein subunit alpha-2

Gene

Ralgapa2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of the heterodimeric RalGAP2 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB.By similarity

GO - Molecular functioni

GO - Biological processi

  • activation of GTPase activity Source: UniProtKB
  • membrane organization Source: Reactome
  • Ral protein signal transduction Source: MGI
  • regulation of exocyst localization Source: MGI
  • regulation of protein localization Source: MGI
  • regulation of small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Ral GTPase-activating protein subunit alpha-2
Alternative name(s):
250 kDa substrate of Akt
Short name:
AS250
P220
Gene namesi
Name:Ralgapa2
Synonyms:Kiaa1272
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:3036245. Ralgapa2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18721872Ral GTPase-activating protein subunit alpha-2PRO_0000286973Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei373 – 3731PhosphoserineCombined sources
Modified residuei376 – 3761PhosphoserineCombined sources
Modified residuei379 – 3791PhosphoserineCombined sources
Modified residuei486 – 4861PhosphoserineCombined sources
Modified residuei696 – 6961Phosphoserine; by PKBBy similarity
Modified residuei715 – 7151Phosphothreonine; by PKBBy similarity
Modified residuei819 – 8191PhosphoserineCombined sources
Modified residuei820 – 8201PhosphoserineCombined sources
Modified residuei1592 – 15921PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA3KGS3.
PaxDbiA3KGS3.
PeptideAtlasiA3KGS3.
PRIDEiA3KGS3.

PTM databases

iPTMnetiA3KGS3.
PhosphoSiteiA3KGS3.

Expressioni

Tissue specificityi

Abundantly expressed in testis, pancreas, lung, thymus, brown fat, and white fat.1 Publication

Gene expression databases

BgeeiENSMUSG00000037110.
CleanExiMM_A230067G21RIK.
ExpressionAtlasiA3KGS3. baseline and differential.

Interactioni

Subunit structurei

Component of the heterodimeric RalGAP2 complex with RALGAPB. Heterodimerization is required for activity (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi232342. 1 interaction.
STRINGi10090.ENSMUSP00000105613.

Structurei

3D structure databases

ProteinModelPortaliA3KGS3.
SMRiA3KGS3. Positions 1629-1843.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1634 – 1842209Rap-GAPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rap-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3686. Eukaryota.
ENOG410XTIX. LUCA.
GeneTreeiENSGT00760000119182.
HOVERGENiHBG051842.
InParanoidiA3KGS3.
PhylomeDBiA3KGS3.
TreeFamiTF324484.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR000331. Rap_GAP_dom.
[Graphical view]
PfamiPF02145. Rap_GAP. 1 hit.
[Graphical view]
SUPFAMiSSF111347. SSF111347. 1 hit.
SSF48371. SSF48371. 1 hit.
PROSITEiPS50085. RAPGAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A3KGS3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFSRRSHGDV KKSTQKVLDP KKDVLTRLKH LRALLDNVDA SDLKQFFETN
60 70 80 90 100
YSQIYFIFYE NFITLENSLK LKGNNKSQRE ELDSILFLFE KILQFLPERI
110 120 130 140 150
FFRWHYQSIG STLKKLLHTG NSIKIRCEGI RLFLLWLQAL QTNCAEEQVL
160 170 180 190 200
IFACLVPGFP AVLSSRGPCT LETLINPSPS IVDAKIYPEE ITPLLPAISG
210 220 230 240 250
EKIAEDQTCF FLQILLKYMV IQAASLEWKN KENQDTGFKF LFTLFRKYYL
260 270 280 290 300
PHLFPSFTKL TNIYKPVLEI PHLRPKPVYV TVTRDNETIY STKIPYMAAR
310 320 330 340 350
VVFIKWIVTF FLEKKYLTAT QNTKNGVDVL PKIIQTVGGG AIQEKVPELD
360 370 380 390 400
GAGSTEQDKS HSNSSTLSDR RLSNSSLCSI EEEHRTVYEM VQRILLSTRG
410 420 430 440 450
YVNFVNEVFR QAFLLPSCEI SVTRKVVQVY RKWILQNKPV FMEEPDKKDV
460 470 480 490 500
AQEDADKLGL SETDSKEASS ESSGHKRSSS WGRTYSFTSA MSRGCVTEED
510 520 530 540 550
NTNVKAGAQA MLQVFLTNAA NVFLLEPCAE VPMLLREQVD ASKAVLIIFR
560 570 580 590 600
RMIMELTMNQ KTWEQMLQIL LRITEAVMQK PKDKHVKDLF AQSLAGLLFR
610 620 630 640 650
TLIVAWIRAN LCVYISRELW DDFLRVLSSL TEWEELITEW SNIMDSLTAV
660 670 680 690 700
LARTVYGVEM TNLPLDKLSE QKEKKQRGKG CILEPQKGTA VGRSFSLSWR
710 720 730 740 750
SHPDVTEPMR FRSATTSGAP GVEKARNTVR QKATEVEEFQ QAESTAAADC
760 770 780 790 800
DYLVVGQQQV PRSSSTSDIT ERLYSDSSQG QKVEHSQNLS SSEPKSVQES
810 820 830 840 850
KGHVTHEHEG ITMLVRRSSS PAELELKDDL QQAHGRCRQR QTSESTGSDT
860 870 880 890 900
VVGYSNEAEL PVSPWQACEE DPDLSTPTDA VADSDARHWL QLSPTDASNL
910 920 930 940 950
TDSRECLADD CSIIAGGNLT GWHPDSAAVL WRRVLGILGD VNNIQSPKIH
960 970 980 990 1000
AKVFGYLYEL WYKLAKIRDN LAISLDNQSS PSPPLLIPPL RMFASWLFKA
1010 1020 1030 1040 1050
TTLPNEYKEG KLQAYKLICA MMTRRQDVLP NSDFLVHFYL VMHLGLTSED
1060 1070 1080 1090 1100
QDVLNTIIKN CSPRFFSLGL PGFSMLVGDF ITAAARVLST DMLAAPRSEA
1110 1120 1130 1140 1150
LTLLGSLVCF PNTYQEIPLL QSVPEVSDVV TGAEDVKHYL INILLKNATE
1160 1170 1180 1190 1200
EPNECARCIA ICSLGVWICE ELAQSASHPQ VKDAINVIGV TLKFPNKIVA
1210 1220 1230 1240 1250
QVACDVLQLL VSYWEKLQMF ETALPRKMAE ILVATIAFLL PSAEYSSVET
1260 1270 1280 1290 1300
DKKFIVSLLL CLLDWCMALP VSALLHPVST AVLEELHPSR APLLDYIYRV
1310 1320 1330 1340 1350
LHCCVCGSST YTQQSHYTLT LADLSSTDYD PFLPLANVRN SEPIQYHSSA
1360 1370 1380 1390 1400
DLGNLLTVEE EKKRRSVELI PLTARMVMAH LVNHLGHYPL SGGPAVLHSL
1410 1420 1430 1440 1450
VSENHDNAHV EGTELSSEVF RSPNLQLFVF NDSTLISYLQ TPAEGPAGGT
1460 1470 1480 1490 1500
SGGSLSDVRV IVRDISGKYS WDGKVLYGPL EGRLAPNGRN PSFQISGWHH
1510 1520 1530 1540 1550
HTCGPQKDLF NGEEGDDVLD KLLENIGHTS PECLLPSQLN LNEPSPTPCA
1560 1570 1580 1590 1600
MNWDQEKAIM EVILRQSAQE DEYVQRCNSD SSVTVTSQGQ PSPVEPRGPF
1610 1620 1630 1640 1650
YFCRLLLDDL GMNSWDRRKN FHLLKKNSKL LRELKNLDSR QCRETHKIAV
1660 1670 1680 1690 1700
FYIAEGQEDK CSILANERGS QAYEDFVAGL GWEVDLSTHC GFMGGLQRNG
1710 1720 1730 1740 1750
STGQTAPYYA TSTVEVIFHV STRMPSDSDD SLTKKLRHLG NDEVHIVWSE
1760 1770 1780 1790 1800
HSRDYRRGII PTAFGDVSII IYPMKNHMFF ITITKKPEVP FFGPLFDGAI
1810 1820 1830 1840 1850
VSGKLLPSLI CATCINASRA VKCLIPLYQS FYEERALYLE AIIQNHREVM
1860 1870
TFEDFAAQVF SPSPSYSVSG TD
Length:1,872
Mass (Da):210,288
Last modified:May 15, 2007 - v2
Checksum:iCCB0AA23790D863A
GO
Isoform 2 (identifier: A3KGS3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1789-1807: VPFFGPLFDGAIVSGKLLP → LLLKIEKFHSLGPCSMERS
     1808-1872: Missing.

Show »
Length:1,807
Mass (Da):203,224
Checksum:i69100CE538A321E1
GO

Sequence cautioni

The sequence BAC30146 differs from that shown.Probable cloning artifact leading to erroneous C-terminus.Curated
The sequence BAC30146 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAM46007 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAM46125 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAM46200 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti436 – 4361Q → R in BAC30146 (PubMed:19468303).Curated
Sequence conflicti447 – 4471K → E in BAE34270 (PubMed:19468303).Curated
Sequence conflicti1511 – 15111N → H in AAH53994 (PubMed:15489334).Curated
Sequence conflicti1727 – 17271D → V in BAD32418 (PubMed:15368895).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1789 – 180719VPFFG…GKLLP → LLLKIEKFHSLGPCSMERS in isoform 2. 1 PublicationVSP_025251Add
BLAST
Alternative sequencei1808 – 187265Missing in isoform 2. 1 PublicationVSP_025252Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL845273, AL845430, AL935056 Genomic DNA. Translation: CAM46125.1. Sequence problems.
AL845273, AL845430, AL935056 Genomic DNA. Translation: CAX15311.1.
AL845430, AL845273, AL935056 Genomic DNA. Translation: CAM46200.1. Sequence problems.
AL845430, AL845273, AL935056 Genomic DNA. Translation: CAX15398.1.
AL935056, AL845273, AL845430 Genomic DNA. Translation: CAM46007.1. Sequence problems.
AL935056, AL845273, AL845430 Genomic DNA. Translation: CAX15696.1.
AK038838 mRNA. Translation: BAC30146.1. Sequence problems.
AK157932 mRNA. Translation: BAE34270.1.
BC053994 mRNA. Translation: AAH53994.1.
BC096528 mRNA. Translation: AAH96528.1.
AK173140 mRNA. Translation: BAD32418.1.
RefSeqiNP_001028520.2. NM_001033348.3.
UniGeneiMm.222310.

Genome annotation databases

EnsembliENSMUST00000131824; ENSMUSP00000122039; ENSMUSG00000037110. [A3KGS3-1]
GeneIDi241694.
KEGGimmu:241694.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL845273, AL845430, AL935056 Genomic DNA. Translation: CAM46125.1. Sequence problems.
AL845273, AL845430, AL935056 Genomic DNA. Translation: CAX15311.1.
AL845430, AL845273, AL935056 Genomic DNA. Translation: CAM46200.1. Sequence problems.
AL845430, AL845273, AL935056 Genomic DNA. Translation: CAX15398.1.
AL935056, AL845273, AL845430 Genomic DNA. Translation: CAM46007.1. Sequence problems.
AL935056, AL845273, AL845430 Genomic DNA. Translation: CAX15696.1.
AK038838 mRNA. Translation: BAC30146.1. Sequence problems.
AK157932 mRNA. Translation: BAE34270.1.
BC053994 mRNA. Translation: AAH53994.1.
BC096528 mRNA. Translation: AAH96528.1.
AK173140 mRNA. Translation: BAD32418.1.
RefSeqiNP_001028520.2. NM_001033348.3.
UniGeneiMm.222310.

3D structure databases

ProteinModelPortaliA3KGS3.
SMRiA3KGS3. Positions 1629-1843.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232342. 1 interaction.
STRINGi10090.ENSMUSP00000105613.

PTM databases

iPTMnetiA3KGS3.
PhosphoSiteiA3KGS3.

Proteomic databases

MaxQBiA3KGS3.
PaxDbiA3KGS3.
PeptideAtlasiA3KGS3.
PRIDEiA3KGS3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000131824; ENSMUSP00000122039; ENSMUSG00000037110. [A3KGS3-1]
GeneIDi241694.
KEGGimmu:241694.

Organism-specific databases

CTDi57186.
MGIiMGI:3036245. Ralgapa2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3686. Eukaryota.
ENOG410XTIX. LUCA.
GeneTreeiENSGT00760000119182.
HOVERGENiHBG051842.
InParanoidiA3KGS3.
PhylomeDBiA3KGS3.
TreeFamiTF324484.

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

ChiTaRSiRalgapa2. mouse.
PROiA3KGS3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037110.
CleanExiMM_A230067G21RIK.
ExpressionAtlasiA3KGS3. baseline and differential.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR000331. Rap_GAP_dom.
[Graphical view]
PfamiPF02145. Rap_GAP. 1 hit.
[Graphical view]
SUPFAMiSSF111347. SSF111347. 1 hit.
SSF48371. SSF48371. 1 hit.
PROSITEiPS50085. RAPGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRGPA2_MOUSE
AccessioniPrimary (citable) accession number: A3KGS3
Secondary accession number(s): A3KGS5
, B7ZCU9, Q3TZD9, Q4VA60, Q69ZM8, Q7TQL4, Q8BYP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: September 7, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.