1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2

Names and origin

Protein names

Recommended name:
    1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2
    EC=3.1.4.11
Alternative name(s):
    Phosphoinositide phospholipase C-beta-2
    Phospholipase C-beta-2
      Short name=PLC-beta-2

Gene names

Name:

Plcb2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	1181 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli By similarity.
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H₂O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	Interacts with RAC1 By similarity.
Miscellaneous	The receptor-mediated activation of PLC-beta-2 is most effectively mediated by one G-protein alpha subunit, alpha-16 By similarity.
Sequence similarities	Contains 1 C2 domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.

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Ontologies

Keywords
Biological process	Lipid degradation
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Transducer
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoinositide phospholipase C activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A3KGF7-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A3KGF7-2) The sequence of this isoform differs from the canonical sequence as follows: 55-77: Missing. 689-702: IISGQFLSERSVRT → ARPRADKWLGGLGS 703-1181: Missing.

Isoform 3 (identifier: A3KGF7-3) The sequence of this isoform differs from the canonical sequence as follows: 689-702: IISGQFLSERSVRT → ARPRADKWLGGLGS 703-1181: Missing.

Isoform 4 (identifier: A3KGF7-4) The sequence of this isoform differs from the canonical sequence as follows: 1-55: Missing. 637-688: DLPMQQNMAL...VVVATTLSIT → GKNTSKTREP...DPYSSLDKEL 689-1181: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1181

1181

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2

PRO_0000295683

Regions

Domain

312 – 463

152

PI-PLC X-box

Domain

547 – 663

117

PI-PLC Y-box

Domain

670 – 768

99

C2

Coiled coil

893 – 940

48

Potential

Coiled coil

974 – 1026

53

Potential

Coiled coil

1075 – 1141

67

Potential

Compositional bias

492 – 546

55

Glu-rich

Sites

Active site

327

1

By similarity

Active site

374

1

By similarity

Metal binding

328

1

Calcium By similarity

Metal binding

357

1

Calcium By similarity

Metal binding

359

1

Calcium By similarity

Metal binding

408

1

Calcium By similarity

Natural variations

Alternative sequence

1 – 55

55

Missing in isoform 4.

VSP_026981

Alternative sequence

55 – 77

23

Missing in isoform 2.

VSP_026982

Alternative sequence

637 – 688

52

DLPMQ…TLSIT → GKNTSKTREPSPGPLTTSPE PSPGPCIPRCPPSEPSSGPC LSDPYSSLDKEL in isoform 4.

VSP_026983

Alternative sequence

689 – 1181

493

Missing in isoform 4.

VSP_026984

Alternative sequence

689 – 702

14

IISGQ…RSVRT → ARPRADKWLGGLGS in isoform 2 and isoform 3.

VSP_026985

Alternative sequence

703 – 1181

479

Missing in isoform 2 and isoform 3.

VSP_026986

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 8E23BD29391ABE4E Show »	FASTA	1,181	134,546
10 20 30 40 50 60 MSLLNPVLLP PNVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT YQNQEMEFLD 70 80 90 100 110 120 VTSIRDTRFG KFAKIPKSQK LREVFNMDFP DNHFLLKTLT VVSGPDMVDL TFYNFVSYKE 130 140 150 160 170 180 NVGKDWAEDV LALAKHPMTV NAPRSTFLDK ILVKLKMQLN PEGKIPVKNF FQMFPADRKR 190 200 210 220 230 240 VEAALGACHL AKGKNDAINP EDFPESVYKS FLMSLCPRPE IDEIFTSYHS KAKPYMTKEH 250 260 270 280 290 300 LTKFINQKQR DPRLNSLLFP PARPEQVQVL IDKYEPSGIN VQRGQLSPEG MVWFLCGPEN 310 320 330 340 350 360 SVLAHDTLLI HQDMTQPLNH YFINSSHNTY LTAGQFSGLS SAEMYRQVLL SGCRCVELDC 370 380 390 400 410 420 WKGKPPDEEP IITHGFTMTT DILFKEAIEA IAESAFKTSP YPVILSFENH VDSPRQQAKM 430 440 450 460 470 480 AEYCRSMFGE TLLTDPLENF PLKPGIPLPS PEDLRGKILI KNKKNQFSGP ASPSKKPGGV 490 500 510 520 530 540 AEGSLPSSVP VEEDTGWTAE DRTEVEEEEV VEEEEEEESG NLDEEEIKKM QSDEGTAGLE 550 560 570 580 590 600 VTAYEEMSSL VNYIQPTKFI SFEFSAQKNR SYVVSSFTEL KAYELLSKAS MQFVDYNKRQ 610 620 630 640 650 660 MSRVYPKGTR MDSSNYMPQM FWNAGCQMVA LNFQTMDLPM QQNMALFEFN GQSGYLLKHE 670 680 690 700 710 720 FMRRLDKQFN PFSVDRIDVV VATTLSITII SGQFLSERSV RTYVEVELFG LPGDPKRRYR 730 740 750 760 770 780 TKLSPTANSI NPVWKEEPFI FEKILMPELA SLRIAVMEEG SKFLGHRIIP INALHSGYHH 790 800 810 820 830 840 LCLRSESNMA LTMPALFVFL EMKDYIPDTW ADLTVALANP IKYFNAQDKK SVKLKGVTGS 850 860 870 880 890 900 LPEKLFSGTP VASQSNGAPV SAGNGSTAPG TKATGEEATK EVTEPQTASL EELRELKGVV 910 920 930 940 950 960 KLQRRHEKEL RELERRGARR WEELLQRGAA QLAELQTQAA GCKLRPGKGS RKKRTLPCEE 970 980 990 1000 1010 1020 TVVAPSEPHD RADPRVQELK DRLEQELQQQ GEEQYRSVLK RKEQHVTEQI AKMMELAREK 1030 1040 1050 1060 1070 1080 QAAELKTFKE TSETDTKEMK KKLEAKRLER IQAMTKVTTD KVAQERLKRE INNSHIQEVV 1090 1100 1110 1120 1130 1140 QAVKQMTETL ERHQEKLEER QTACLEQIQA MEKQFQEKAL AEYEAKMKGL EAEVKESVRA 1150 1160 1170 1180 YFKDCFPTEA EDKPERSCEA SEESCPQEPL VSKADTQESR L « Hide
Isoform 2. Checksum: 671A64A042AE7024 Show »	FASTA	679	77,235
10 20 30 40 50 60 MSLLNPVLLP PNVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT YQNQSQKLRE 70 80 90 100 110 120 VFNMDFPDNH FLLKTLTVVS GPDMVDLTFY NFVSYKENVG KDWAEDVLAL AKHPMTVNAP 130 140 150 160 170 180 RSTFLDKILV KLKMQLNPEG KIPVKNFFQM FPADRKRVEA ALGACHLAKG KNDAINPEDF 190 200 210 220 230 240 PESVYKSFLM SLCPRPEIDE IFTSYHSKAK PYMTKEHLTK FINQKQRDPR LNSLLFPPAR 250 260 270 280 290 300 PEQVQVLIDK YEPSGINVQR GQLSPEGMVW FLCGPENSVL AHDTLLIHQD MTQPLNHYFI 310 320 330 340 350 360 NSSHNTYLTA GQFSGLSSAE MYRQVLLSGC RCVELDCWKG KPPDEEPIIT HGFTMTTDIL 370 380 390 400 410 420 FKEAIEAIAE SAFKTSPYPV ILSFENHVDS PRQQAKMAEY CRSMFGETLL TDPLENFPLK 430 440 450 460 470 480 PGIPLPSPED LRGKILIKNK KNQFSGPASP SKKPGGVAEG SLPSSVPVEE DTGWTAEDRT 490 500 510 520 530 540 EVEEEEVVEE EEEEESGNLD EEEIKKMQSD EGTAGLEVTA YEEMSSLVNY IQPTKFISFE 550 560 570 580 590 600 FSAQKNRSYV VSSFTELKAY ELLSKASMQF VDYNKRQMSR VYPKGTRMDS SNYMPQMFWN 610 620 630 640 650 660 AGCQMVALNF QTMDLPMQQN MALFEFNGQS GYLLKHEFMR RLDKQFNPFS VDRIDVVVAT 670 TLSITARPRA DKWLGGLGS « Hide
Isoform 3. Checksum: B07FB842B2C8CC73 Show »	FASTA	702	79,946
10 20 30 40 50 60 MSLLNPVLLP PNVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT YQNQEMEFLD 70 80 90 100 110 120 VTSIRDTRFG KFAKIPKSQK LREVFNMDFP DNHFLLKTLT VVSGPDMVDL TFYNFVSYKE 130 140 150 160 170 180 NVGKDWAEDV LALAKHPMTV NAPRSTFLDK ILVKLKMQLN PEGKIPVKNF FQMFPADRKR 190 200 210 220 230 240 VEAALGACHL AKGKNDAINP EDFPESVYKS FLMSLCPRPE IDEIFTSYHS KAKPYMTKEH 250 260 270 280 290 300 LTKFINQKQR DPRLNSLLFP PARPEQVQVL IDKYEPSGIN VQRGQLSPEG MVWFLCGPEN 310 320 330 340 350 360 SVLAHDTLLI HQDMTQPLNH YFINSSHNTY LTAGQFSGLS SAEMYRQVLL SGCRCVELDC 370 380 390 400 410 420 WKGKPPDEEP IITHGFTMTT DILFKEAIEA IAESAFKTSP YPVILSFENH VDSPRQQAKM 430 440 450 460 470 480 AEYCRSMFGE TLLTDPLENF PLKPGIPLPS PEDLRGKILI KNKKNQFSGP ASPSKKPGGV 490 500 510 520 530 540 AEGSLPSSVP VEEDTGWTAE DRTEVEEEEV VEEEEEEESG NLDEEEIKKM QSDEGTAGLE 550 560 570 580 590 600 VTAYEEMSSL VNYIQPTKFI SFEFSAQKNR SYVVSSFTEL KAYELLSKAS MQFVDYNKRQ 610 620 630 640 650 660 MSRVYPKGTR MDSSNYMPQM FWNAGCQMVA LNFQTMDLPM QQNMALFEFN GQSGYLLKHE 670 680 690 700 FMRRLDKQFN PFSVDRIDVV VATTLSITAR PRADKWLGGL GS « Hide
Isoform 4. Checksum: 30D14CB6B1901634 Show »	FASTA	633	71,354
10 20 30 40 50 60 MEFLDVTSIR DTRFGKFAKI PKSQKLREVF NMDFPDNHFL LKTLTVVSGP DMVDLTFYNF 70 80 90 100 110 120 VSYKENVGKD WAEDVLALAK HPMTVNAPRS TFLDKILVKL KMQLNPEGKI PVKNFFQMFP 130 140 150 160 170 180 ADRKRVEAAL GACHLAKGKN DAINPEDFPE SVYKSFLMSL CPRPEIDEIF TSYHSKAKPY 190 200 210 220 230 240 MTKEHLTKFI NQKQRDPRLN SLLFPPARPE QVQVLIDKYE PSGINVQRGQ LSPEGMVWFL 250 260 270 280 290 300 CGPENSVLAH DTLLIHQDMT QPLNHYFINS SHNTYLTAGQ FSGLSSAEMY RQVLLSGCRC 310 320 330 340 350 360 VELDCWKGKP PDEEPIITHG FTMTTDILFK EAIEAIAESA FKTSPYPVIL SFENHVDSPR 370 380 390 400 410 420 QQAKMAEYCR SMFGETLLTD PLENFPLKPG IPLPSPEDLR GKILIKNKKN QFSGPASPSK 430 440 450 460 470 480 KPGGVAEGSL PSSVPVEEDT GWTAEDRTEV EEEEVVEEEE EEESGNLDEE EIKKMQSDEG 490 500 510 520 530 540 TAGLEVTAYE EMSSLVNYIQ PTKFISFEFS AQKNRSYVVS SFTELKAYEL LSKASMQFVD 550 560 570 580 590 600 YNKRQMSRVY PKGTRMDSSN YMPQMFWNAG CQMVALNFQT MGKNTSKTRE PSPGPLTTSP 610 620 630 EPSPGPCIPR CPPSEPSSGP CLSDPYSSLD KEL « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of quantitative trait loci influencing growth and adiposity using congenic mouse strains." Farber C.R., Corva P.M., Medrano J.F. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 3).
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4). Strain: C57BL/6J. Tissue: Corpora quadrigemina and Thymus.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: C57BL/6J.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AY902324 Genomic DNA. Translation: AAX90609.1. AK045469 mRNA. Translation: BAC32384.1. AK169442 mRNA. Translation: BAE41180.1. AL772255 Genomic DNA. Translation: CAM45993.1. AL772255 Genomic DNA. Translation: CAM45994.1. AL772255 Genomic DNA. Translation: CAM45995.1.
IPI	IPI00222032. IPI00652801. IPI00756159. IPI00855131.
RefSeq	NP_808236.2.
UniGene	Mm.215156
3D structure databases
SMR	A3KGF7. Positions 11-804, 878-1147.
ModBase	Search...
Protein-protein interaction databases
STRING	A3KGF7.
PTM databases
PhosphoSite	A3KGF7.
Proteomic databases
PRIDE	A3KGF7.
Genome annotation databases
Ensembl	ENSMUST00000006415; ENSMUSP00000006415; ENSMUSG00000040061; Mus musculus. [Genome view] ENSMUST00000102524; ENSMUSP00000099583; ENSMUSG00000040061; Mus musculus. [Genome view]
GeneID	18796.
KEGG	mmu:18796.
Organism-specific databases
CTD	18796.
MGI	MGI:107465. Plcb2.
Phylogenomic databases
HOGENOM	HBG315986.
InParanoid	A3KGF7.
OMA	VKAYLSQ.
OrthoDB	EOG9HQH4M.
PhylomeDB	A3KGF7.
Enzyme and pathway databases
BRENDA	3.1.4.11. 244.
Gene expression databases
Bgee	A3KGF7.
CleanEx	MM_PLCB2.
Genevestigator	A3KGF7.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR011992. EF-hand-like_dom. IPR015359. Phospholipase_C_EF-hand-like. IPR001192. Phospholipase_C_Pinositol-sp_C. IPR001711. Phospholipase_C_Pinositol-sp_Y. IPR016280. PLC-beta. IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR000909. PLipase_C_PInositol-sp_X_dom. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit. G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 1 hit.
Pfam	PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PIRSF	PIRSF000956. PLC-beta. 1 hit.
PRINTS	PR00390. PHPHLIPASEC.
SMART	SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
PROSITE	PS50004. C2. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	295084.
SOURCE	Search...

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Entry information

Entry name

PLCB2_MOUSE

Accession

Primary (citable) accession number: A3KGF7
Secondary accession number(s): Q2M4J1, Q3TER8, Q8BI81

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	April 3, 2007
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents