Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

Gene

Plcb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli (By similarity).By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei327PROSITE-ProRule annotation1
Metal bindingi328CalciumBy similarity1
Metal bindingi357CalciumBy similarity1
Metal bindingi359CalciumBy similarity1
Active sitei374PROSITE-ProRule annotation1
Metal bindingi408CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • intracellular signal transduction Source: InterPro
  • lipid catabolic process Source: UniProtKB-KW
  • sensory perception of bitter taste Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-112043. PLC beta mediated events.
R-MMU-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-MMU-399997. Acetylcholine regulates insulin secretion.
R-MMU-4086398. Ca2+ pathway.
R-MMU-416476. G alpha (q) signalling events.
R-MMU-418217. G beta:gamma signalling through PLC beta.
R-MMU-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-MMU-500657. Presynaptic function of Kainate receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-beta-2
Phospholipase C-beta-2
Short name:
PLC-beta-2
Gene namesi
Name:Plcb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:107465. Plcb2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002956831 – 11811-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2Add BLAST1181

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei950PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiA3KGF7.
PaxDbiA3KGF7.
PeptideAtlasiA3KGF7.
PRIDEiA3KGF7.

PTM databases

iPTMnetiA3KGF7.
PhosphoSitePlusiA3KGF7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040061.
CleanExiMM_PLCB2.
ExpressionAtlasiA3KGF7. baseline and differential.
GenevisibleiA3KGF7. MM.

Interactioni

Subunit structurei

Interacts with RAC1.By similarity

Protein-protein interaction databases

IntActiA3KGF7. 6 interactors.
STRINGi10090.ENSMUSP00000099583.

Structurei

3D structure databases

ProteinModelPortaliA3KGF7.
SMRiA3KGF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini312 – 463PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST152
Domaini547 – 663PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini670 – 768C2PROSITE-ProRule annotationAdd BLAST99

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili893 – 940Sequence analysisAdd BLAST48
Coiled coili974 – 1026Sequence analysisAdd BLAST53
Coiled coili1075 – 1141Sequence analysisAdd BLAST67

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi492 – 546Glu-richAdd BLAST55

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000232046.
HOVERGENiHBG053609.
InParanoidiA3KGF7.
KOiK05858.
OMAiYEPSGIN.
OrthoDBiEOG091G00XL.
PhylomeDBiA3KGF7.
TreeFamiTF313216.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR011993. PH_dom-like.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028403. PLC-beta2.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLC_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF10. PTHR10336:SF10. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000956. PLC-beta. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A3KGF7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLNPVLLP PNVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT
60 70 80 90 100
YQNQEMEFLD VTSIRDTRFG KFAKIPKSQK LREVFNMDFP DNHFLLKTLT
110 120 130 140 150
VVSGPDMVDL TFYNFVSYKE NVGKDWAEDV LALAKHPMTV NAPRSTFLDK
160 170 180 190 200
ILVKLKMQLN PEGKIPVKNF FQMFPADRKR VEAALGACHL AKGKNDAINP
210 220 230 240 250
EDFPESVYKS FLMSLCPRPE IDEIFTSYHS KAKPYMTKEH LTKFINQKQR
260 270 280 290 300
DPRLNSLLFP PARPEQVQVL IDKYEPSGIN VQRGQLSPEG MVWFLCGPEN
310 320 330 340 350
SVLAHDTLLI HQDMTQPLNH YFINSSHNTY LTAGQFSGLS SAEMYRQVLL
360 370 380 390 400
SGCRCVELDC WKGKPPDEEP IITHGFTMTT DILFKEAIEA IAESAFKTSP
410 420 430 440 450
YPVILSFENH VDSPRQQAKM AEYCRSMFGE TLLTDPLENF PLKPGIPLPS
460 470 480 490 500
PEDLRGKILI KNKKNQFSGP ASPSKKPGGV AEGSLPSSVP VEEDTGWTAE
510 520 530 540 550
DRTEVEEEEV VEEEEEEESG NLDEEEIKKM QSDEGTAGLE VTAYEEMSSL
560 570 580 590 600
VNYIQPTKFI SFEFSAQKNR SYVVSSFTEL KAYELLSKAS MQFVDYNKRQ
610 620 630 640 650
MSRVYPKGTR MDSSNYMPQM FWNAGCQMVA LNFQTMDLPM QQNMALFEFN
660 670 680 690 700
GQSGYLLKHE FMRRLDKQFN PFSVDRIDVV VATTLSITII SGQFLSERSV
710 720 730 740 750
RTYVEVELFG LPGDPKRRYR TKLSPTANSI NPVWKEEPFI FEKILMPELA
760 770 780 790 800
SLRIAVMEEG SKFLGHRIIP INALHSGYHH LCLRSESNMA LTMPALFVFL
810 820 830 840 850
EMKDYIPDTW ADLTVALANP IKYFNAQDKK SVKLKGVTGS LPEKLFSGTP
860 870 880 890 900
VASQSNGAPV SAGNGSTAPG TKATGEEATK EVTEPQTASL EELRELKGVV
910 920 930 940 950
KLQRRHEKEL RELERRGARR WEELLQRGAA QLAELQTQAA GCKLRPGKGS
960 970 980 990 1000
RKKRTLPCEE TVVAPSEPHD RADPRVQELK DRLEQELQQQ GEEQYRSVLK
1010 1020 1030 1040 1050
RKEQHVTEQI AKMMELAREK QAAELKTFKE TSETDTKEMK KKLEAKRLER
1060 1070 1080 1090 1100
IQAMTKVTTD KVAQERLKRE INNSHIQEVV QAVKQMTETL ERHQEKLEER
1110 1120 1130 1140 1150
QTACLEQIQA MEKQFQEKAL AEYEAKMKGL EAEVKESVRA YFKDCFPTEA
1160 1170 1180
EDKPERSCEA SEESCPQEPL VSKADTQESR L
Length:1,181
Mass (Da):134,546
Last modified:April 3, 2007 - v1
Checksum:i8E23BD29391ABE4E
GO
Isoform 2 (identifier: A3KGF7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     55-77: Missing.
     689-702: IISGQFLSERSVRT → ARPRADKWLGGLGS
     703-1181: Missing.

Show »
Length:679
Mass (Da):77,235
Checksum:i671A64A042AE7024
GO
Isoform 3 (identifier: A3KGF7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     689-702: IISGQFLSERSVRT → ARPRADKWLGGLGS
     703-1181: Missing.

Show »
Length:702
Mass (Da):79,946
Checksum:iB07FB842B2C8CC73
GO
Isoform 4 (identifier: A3KGF7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.
     637-688: DLPMQQNMAL...VVVATTLSIT → GKNTSKTREP...DPYSSLDKEL
     689-1181: Missing.

Show »
Length:633
Mass (Da):71,354
Checksum:i30D14CB6B1901634
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0269811 – 55Missing in isoform 4. 1 PublicationAdd BLAST55
Alternative sequenceiVSP_02698255 – 77Missing in isoform 2. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_026983637 – 688DLPMQ…TLSIT → GKNTSKTREPSPGPLTTSPE PSPGPCIPRCPPSEPSSGPC LSDPYSSLDKEL in isoform 4. 1 PublicationAdd BLAST52
Alternative sequenceiVSP_026984689 – 1181Missing in isoform 4. 1 PublicationAdd BLAST493
Alternative sequenceiVSP_026985689 – 702IISGQ…RSVRT → ARPRADKWLGGLGS in isoform 2 and isoform 3. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_026986703 – 1181Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST479

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY902324 Genomic DNA. Translation: AAX90609.1.
AK045469 mRNA. Translation: BAC32384.1.
AK169442 mRNA. Translation: BAE41180.1.
AL772255 Genomic DNA. Translation: CAM45993.1.
AL772255 Genomic DNA. Translation: CAM45994.1.
AL772255 Genomic DNA. Translation: CAM45995.1.
CCDSiCCDS16582.1. [A3KGF7-1]
RefSeqiNP_001277719.1. NM_001290790.1.
NP_808236.2. NM_177568.2. [A3KGF7-1]
UniGeneiMm.215156.

Genome annotation databases

EnsembliENSMUST00000102524; ENSMUSP00000099583; ENSMUSG00000040061. [A3KGF7-1]
GeneIDi18796.
KEGGimmu:18796.
UCSCiuc008lsi.1. mouse. [A3KGF7-1]
uc008lsk.1. mouse. [A3KGF7-2]
uc008lsl.1. mouse. [A3KGF7-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY902324 Genomic DNA. Translation: AAX90609.1.
AK045469 mRNA. Translation: BAC32384.1.
AK169442 mRNA. Translation: BAE41180.1.
AL772255 Genomic DNA. Translation: CAM45993.1.
AL772255 Genomic DNA. Translation: CAM45994.1.
AL772255 Genomic DNA. Translation: CAM45995.1.
CCDSiCCDS16582.1. [A3KGF7-1]
RefSeqiNP_001277719.1. NM_001290790.1.
NP_808236.2. NM_177568.2. [A3KGF7-1]
UniGeneiMm.215156.

3D structure databases

ProteinModelPortaliA3KGF7.
SMRiA3KGF7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiA3KGF7. 6 interactors.
STRINGi10090.ENSMUSP00000099583.

PTM databases

iPTMnetiA3KGF7.
PhosphoSitePlusiA3KGF7.

Proteomic databases

EPDiA3KGF7.
PaxDbiA3KGF7.
PeptideAtlasiA3KGF7.
PRIDEiA3KGF7.

Protocols and materials databases

DNASUi18796.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102524; ENSMUSP00000099583; ENSMUSG00000040061. [A3KGF7-1]
GeneIDi18796.
KEGGimmu:18796.
UCSCiuc008lsi.1. mouse. [A3KGF7-1]
uc008lsk.1. mouse. [A3KGF7-2]
uc008lsl.1. mouse. [A3KGF7-4]

Organism-specific databases

CTDi5330.
MGIiMGI:107465. Plcb2.

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000232046.
HOVERGENiHBG053609.
InParanoidiA3KGF7.
KOiK05858.
OMAiYEPSGIN.
OrthoDBiEOG091G00XL.
PhylomeDBiA3KGF7.
TreeFamiTF313216.

Enzyme and pathway databases

ReactomeiR-MMU-112043. PLC beta mediated events.
R-MMU-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-MMU-399997. Acetylcholine regulates insulin secretion.
R-MMU-4086398. Ca2+ pathway.
R-MMU-416476. G alpha (q) signalling events.
R-MMU-418217. G beta:gamma signalling through PLC beta.
R-MMU-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-MMU-500657. Presynaptic function of Kainate receptors.

Miscellaneous databases

ChiTaRSiPlcb2. mouse.
PROiA3KGF7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040061.
CleanExiMM_PLCB2.
ExpressionAtlasiA3KGF7. baseline and differential.
GenevisibleiA3KGF7. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR011993. PH_dom-like.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028403. PLC-beta2.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLC_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF10. PTHR10336:SF10. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000956. PLC-beta. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLCB2_MOUSE
AccessioniPrimary (citable) accession number: A3KGF7
Secondary accession number(s): Q2M4J1, Q3TER8, Q8BI81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: April 3, 2007
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The receptor-mediated activation of PLC-beta-2 is most effectively mediated by one G-protein alpha subunit, alpha-16.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.