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A3KGF7

- PLCB2_MOUSE

UniProt

A3KGF7 - PLCB2_MOUSE

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

Gene

Plcb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (03 Apr 2007)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli By similarity.By similarity

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei327 – 3271PROSITE-ProRule annotation
    Metal bindingi328 – 3281CalciumBy similarity
    Metal bindingi357 – 3571CalciumBy similarity
    Metal bindingi359 – 3591CalciumBy similarity
    Active sitei374 – 3741PROSITE-ProRule annotation
    Metal bindingi408 – 4081CalciumBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. lipid catabolic process Source: UniProtKB-KW
    3. sensory perception of bitter taste Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196473. Synthesis of IP3 and IP4 in the cytosol.
    REACT_198612. Presynaptic function of Kainate receptors.
    REACT_207651. G alpha (q) signalling events.
    REACT_221970. Ca2+ pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C-beta-2
    Phospholipase C-beta-2
    Short name:
    PLC-beta-2
    Gene namesi
    Name:Plcb2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:107465. Plcb2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 118111811-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2PRO_0000295683Add
    BLAST

    Proteomic databases

    PaxDbiA3KGF7.
    PRIDEiA3KGF7.

    PTM databases

    PhosphoSiteiA3KGF7.

    Expressioni

    Gene expression databases

    ArrayExpressiA3KGF7.
    BgeeiA3KGF7.
    CleanExiMM_PLCB2.
    GenevestigatoriA3KGF7.

    Interactioni

    Subunit structurei

    Interacts with RAC1.By similarity

    Protein-protein interaction databases

    IntActiA3KGF7. 6 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliA3KGF7.
    SMRiA3KGF7. Positions 11-834, 880-1147.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini312 – 463152PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini547 – 663117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini670 – 76899C2PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili893 – 94048Sequence AnalysisAdd
    BLAST
    Coiled coili974 – 102653Sequence AnalysisAdd
    BLAST
    Coiled coili1075 – 114167Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi492 – 54655Glu-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG149692.
    GeneTreeiENSGT00730000110266.
    HOGENOMiHOG000232046.
    HOVERGENiHBG053609.
    InParanoidiA3KGF7.
    KOiK05858.
    OMAiFIKWDDE.
    OrthoDBiEOG7WDN1N.
    PhylomeDBiA3KGF7.
    TreeFamiTF313216.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR016280. PLC-beta.
    IPR028403. PLC-beta2.
    IPR014815. PLC-beta_C.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF10. PTHR10336:SF10. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    PF08703. PLC-beta_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000956. PLC-beta. 1 hit.
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: A3KGF7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLLNPVLLP PNVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT     50
    YQNQEMEFLD VTSIRDTRFG KFAKIPKSQK LREVFNMDFP DNHFLLKTLT 100
    VVSGPDMVDL TFYNFVSYKE NVGKDWAEDV LALAKHPMTV NAPRSTFLDK 150
    ILVKLKMQLN PEGKIPVKNF FQMFPADRKR VEAALGACHL AKGKNDAINP 200
    EDFPESVYKS FLMSLCPRPE IDEIFTSYHS KAKPYMTKEH LTKFINQKQR 250
    DPRLNSLLFP PARPEQVQVL IDKYEPSGIN VQRGQLSPEG MVWFLCGPEN 300
    SVLAHDTLLI HQDMTQPLNH YFINSSHNTY LTAGQFSGLS SAEMYRQVLL 350
    SGCRCVELDC WKGKPPDEEP IITHGFTMTT DILFKEAIEA IAESAFKTSP 400
    YPVILSFENH VDSPRQQAKM AEYCRSMFGE TLLTDPLENF PLKPGIPLPS 450
    PEDLRGKILI KNKKNQFSGP ASPSKKPGGV AEGSLPSSVP VEEDTGWTAE 500
    DRTEVEEEEV VEEEEEEESG NLDEEEIKKM QSDEGTAGLE VTAYEEMSSL 550
    VNYIQPTKFI SFEFSAQKNR SYVVSSFTEL KAYELLSKAS MQFVDYNKRQ 600
    MSRVYPKGTR MDSSNYMPQM FWNAGCQMVA LNFQTMDLPM QQNMALFEFN 650
    GQSGYLLKHE FMRRLDKQFN PFSVDRIDVV VATTLSITII SGQFLSERSV 700
    RTYVEVELFG LPGDPKRRYR TKLSPTANSI NPVWKEEPFI FEKILMPELA 750
    SLRIAVMEEG SKFLGHRIIP INALHSGYHH LCLRSESNMA LTMPALFVFL 800
    EMKDYIPDTW ADLTVALANP IKYFNAQDKK SVKLKGVTGS LPEKLFSGTP 850
    VASQSNGAPV SAGNGSTAPG TKATGEEATK EVTEPQTASL EELRELKGVV 900
    KLQRRHEKEL RELERRGARR WEELLQRGAA QLAELQTQAA GCKLRPGKGS 950
    RKKRTLPCEE TVVAPSEPHD RADPRVQELK DRLEQELQQQ GEEQYRSVLK 1000
    RKEQHVTEQI AKMMELAREK QAAELKTFKE TSETDTKEMK KKLEAKRLER 1050
    IQAMTKVTTD KVAQERLKRE INNSHIQEVV QAVKQMTETL ERHQEKLEER 1100
    QTACLEQIQA MEKQFQEKAL AEYEAKMKGL EAEVKESVRA YFKDCFPTEA 1150
    EDKPERSCEA SEESCPQEPL VSKADTQESR L 1181
    Length:1,181
    Mass (Da):134,546
    Last modified:April 3, 2007 - v1
    Checksum:i8E23BD29391ABE4E
    GO
    Isoform 2 (identifier: A3KGF7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         55-77: Missing.
         689-702: IISGQFLSERSVRT → ARPRADKWLGGLGS
         703-1181: Missing.

    Show »
    Length:679
    Mass (Da):77,235
    Checksum:i671A64A042AE7024
    GO
    Isoform 3 (identifier: A3KGF7-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         689-702: IISGQFLSERSVRT → ARPRADKWLGGLGS
         703-1181: Missing.

    Show »
    Length:702
    Mass (Da):79,946
    Checksum:iB07FB842B2C8CC73
    GO
    Isoform 4 (identifier: A3KGF7-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-55: Missing.
         637-688: DLPMQQNMAL...VVVATTLSIT → GKNTSKTREP...DPYSSLDKEL
         689-1181: Missing.

    Show »
    Length:633
    Mass (Da):71,354
    Checksum:i30D14CB6B1901634
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5555Missing in isoform 4. 1 PublicationVSP_026981Add
    BLAST
    Alternative sequencei55 – 7723Missing in isoform 2. 1 PublicationVSP_026982Add
    BLAST
    Alternative sequencei637 – 68852DLPMQ…TLSIT → GKNTSKTREPSPGPLTTSPE PSPGPCIPRCPPSEPSSGPC LSDPYSSLDKEL in isoform 4. 1 PublicationVSP_026983Add
    BLAST
    Alternative sequencei689 – 1181493Missing in isoform 4. 1 PublicationVSP_026984Add
    BLAST
    Alternative sequencei689 – 70214IISGQ…RSVRT → ARPRADKWLGGLGS in isoform 2 and isoform 3. 1 PublicationVSP_026985Add
    BLAST
    Alternative sequencei703 – 1181479Missing in isoform 2 and isoform 3. 1 PublicationVSP_026986Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY902324 Genomic DNA. Translation: AAX90609.1.
    AK045469 mRNA. Translation: BAC32384.1.
    AK169442 mRNA. Translation: BAE41180.1.
    AL772255 Genomic DNA. Translation: CAM45993.1.
    AL772255 Genomic DNA. Translation: CAM45994.1.
    AL772255 Genomic DNA. Translation: CAM45995.1.
    CCDSiCCDS16582.1. [A3KGF7-1]
    RefSeqiNP_001277719.1. NM_001290790.1.
    NP_808236.2. NM_177568.2. [A3KGF7-1]
    UniGeneiMm.215156.

    Genome annotation databases

    EnsembliENSMUST00000102524; ENSMUSP00000099583; ENSMUSG00000040061. [A3KGF7-1]
    GeneIDi18796.
    KEGGimmu:18796.
    UCSCiuc008lsi.1. mouse. [A3KGF7-1]
    uc008lsk.1. mouse. [A3KGF7-2]
    uc008lsl.1. mouse. [A3KGF7-4]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY902324 Genomic DNA. Translation: AAX90609.1 .
    AK045469 mRNA. Translation: BAC32384.1 .
    AK169442 mRNA. Translation: BAE41180.1 .
    AL772255 Genomic DNA. Translation: CAM45993.1 .
    AL772255 Genomic DNA. Translation: CAM45994.1 .
    AL772255 Genomic DNA. Translation: CAM45995.1 .
    CCDSi CCDS16582.1. [A3KGF7-1 ]
    RefSeqi NP_001277719.1. NM_001290790.1.
    NP_808236.2. NM_177568.2. [A3KGF7-1 ]
    UniGenei Mm.215156.

    3D structure databases

    ProteinModelPortali A3KGF7.
    SMRi A3KGF7. Positions 11-834, 880-1147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi A3KGF7. 6 interactions.

    PTM databases

    PhosphoSitei A3KGF7.

    Proteomic databases

    PaxDbi A3KGF7.
    PRIDEi A3KGF7.

    Protocols and materials databases

    DNASUi 18796.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102524 ; ENSMUSP00000099583 ; ENSMUSG00000040061 . [A3KGF7-1 ]
    GeneIDi 18796.
    KEGGi mmu:18796.
    UCSCi uc008lsi.1. mouse. [A3KGF7-1 ]
    uc008lsk.1. mouse. [A3KGF7-2 ]
    uc008lsl.1. mouse. [A3KGF7-4 ]

    Organism-specific databases

    CTDi 5330.
    MGIi MGI:107465. Plcb2.

    Phylogenomic databases

    eggNOGi NOG149692.
    GeneTreei ENSGT00730000110266.
    HOGENOMi HOG000232046.
    HOVERGENi HBG053609.
    InParanoidi A3KGF7.
    KOi K05858.
    OMAi FIKWDDE.
    OrthoDBi EOG7WDN1N.
    PhylomeDBi A3KGF7.
    TreeFami TF313216.

    Enzyme and pathway databases

    Reactomei REACT_196473. Synthesis of IP3 and IP4 in the cytosol.
    REACT_198612. Presynaptic function of Kainate receptors.
    REACT_207651. G alpha (q) signalling events.
    REACT_221970. Ca2+ pathway.

    Miscellaneous databases

    ChiTaRSi PLCB2. mouse.
    NextBioi 295084.
    PROi A3KGF7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi A3KGF7.
    Bgeei A3KGF7.
    CleanExi MM_PLCB2.
    Genevestigatori A3KGF7.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR016280. PLC-beta.
    IPR028403. PLC-beta2.
    IPR014815. PLC-beta_C.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF10. PTHR10336:SF10. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    PF08703. PLC-beta_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000956. PLC-beta. 1 hit.
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of quantitative trait loci influencing growth and adiposity using congenic mouse strains."
      Farber C.R., Corva P.M., Medrano J.F.
      Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 3).
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
      Strain: C57BL/6J.
      Tissue: Corpora quadrigemina and Thymus.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiPLCB2_MOUSE
    AccessioniPrimary (citable) accession number: A3KGF7
    Secondary accession number(s): Q2M4J1, Q3TER8, Q8BI81
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2007
    Last sequence update: April 3, 2007
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The receptor-mediated activation of PLC-beta-2 is most effectively mediated by one G-protein alpha subunit, alpha-16.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3