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A3KGF7

- PLCB2_MOUSE

UniProt

A3KGF7 - PLCB2_MOUSE

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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

Gene
Plcb2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli By similarity.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Binds 1 calcium ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei327 – 3271 By similarity
Metal bindingi328 – 3281Calcium By similarity
Metal bindingi357 – 3571Calcium By similarity
Metal bindingi359 – 3591Calcium By similarity
Active sitei374 – 3741 By similarity
Metal bindingi408 – 4081Calcium By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. lipid catabolic process Source: UniProtKB-KW
  3. sensory perception of bitter taste Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196473. Synthesis of IP3 and IP4 in the cytosol.
REACT_198612. Presynaptic function of Kainate receptors.
REACT_207651. G alpha (q) signalling events.
REACT_221970. Ca2+ pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-beta-2
Phospholipase C-beta-2
Short name:
PLC-beta-2
Gene namesi
Name:Plcb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:107465. Plcb2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 118111811-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2PRO_0000295683Add
BLAST

Proteomic databases

PaxDbiA3KGF7.
PRIDEiA3KGF7.

PTM databases

PhosphoSiteiA3KGF7.

Expressioni

Gene expression databases

ArrayExpressiA3KGF7.
BgeeiA3KGF7.
CleanExiMM_PLCB2.
GenevestigatoriA3KGF7.

Interactioni

Subunit structurei

Interacts with RAC1 By similarity.

Protein-protein interaction databases

IntActiA3KGF7. 6 interactions.

Structurei

3D structure databases

ProteinModelPortaliA3KGF7.
SMRiA3KGF7. Positions 11-834, 880-1147.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini312 – 463152PI-PLC X-boxAdd
BLAST
Domaini547 – 663117PI-PLC Y-boxAdd
BLAST
Domaini670 – 76899C2Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili893 – 94048 Reviewed predictionAdd
BLAST
Coiled coili974 – 102653 Reviewed predictionAdd
BLAST
Coiled coili1075 – 114167 Reviewed predictionAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi492 – 54655Glu-richAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00730000110266.
HOGENOMiHOG000232046.
HOVERGENiHBG053609.
InParanoidiA3KGF7.
KOiK05858.
OMAiFIKWDDE.
OrthoDBiEOG7WDN1N.
PhylomeDBiA3KGF7.
TreeFamiTF313216.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028403. PLC-beta2.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF10. PTHR10336:SF10. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000956. PLC-beta. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: A3KGF7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSLLNPVLLP PNVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT     50
YQNQEMEFLD VTSIRDTRFG KFAKIPKSQK LREVFNMDFP DNHFLLKTLT 100
VVSGPDMVDL TFYNFVSYKE NVGKDWAEDV LALAKHPMTV NAPRSTFLDK 150
ILVKLKMQLN PEGKIPVKNF FQMFPADRKR VEAALGACHL AKGKNDAINP 200
EDFPESVYKS FLMSLCPRPE IDEIFTSYHS KAKPYMTKEH LTKFINQKQR 250
DPRLNSLLFP PARPEQVQVL IDKYEPSGIN VQRGQLSPEG MVWFLCGPEN 300
SVLAHDTLLI HQDMTQPLNH YFINSSHNTY LTAGQFSGLS SAEMYRQVLL 350
SGCRCVELDC WKGKPPDEEP IITHGFTMTT DILFKEAIEA IAESAFKTSP 400
YPVILSFENH VDSPRQQAKM AEYCRSMFGE TLLTDPLENF PLKPGIPLPS 450
PEDLRGKILI KNKKNQFSGP ASPSKKPGGV AEGSLPSSVP VEEDTGWTAE 500
DRTEVEEEEV VEEEEEEESG NLDEEEIKKM QSDEGTAGLE VTAYEEMSSL 550
VNYIQPTKFI SFEFSAQKNR SYVVSSFTEL KAYELLSKAS MQFVDYNKRQ 600
MSRVYPKGTR MDSSNYMPQM FWNAGCQMVA LNFQTMDLPM QQNMALFEFN 650
GQSGYLLKHE FMRRLDKQFN PFSVDRIDVV VATTLSITII SGQFLSERSV 700
RTYVEVELFG LPGDPKRRYR TKLSPTANSI NPVWKEEPFI FEKILMPELA 750
SLRIAVMEEG SKFLGHRIIP INALHSGYHH LCLRSESNMA LTMPALFVFL 800
EMKDYIPDTW ADLTVALANP IKYFNAQDKK SVKLKGVTGS LPEKLFSGTP 850
VASQSNGAPV SAGNGSTAPG TKATGEEATK EVTEPQTASL EELRELKGVV 900
KLQRRHEKEL RELERRGARR WEELLQRGAA QLAELQTQAA GCKLRPGKGS 950
RKKRTLPCEE TVVAPSEPHD RADPRVQELK DRLEQELQQQ GEEQYRSVLK 1000
RKEQHVTEQI AKMMELAREK QAAELKTFKE TSETDTKEMK KKLEAKRLER 1050
IQAMTKVTTD KVAQERLKRE INNSHIQEVV QAVKQMTETL ERHQEKLEER 1100
QTACLEQIQA MEKQFQEKAL AEYEAKMKGL EAEVKESVRA YFKDCFPTEA 1150
EDKPERSCEA SEESCPQEPL VSKADTQESR L 1181
Length:1,181
Mass (Da):134,546
Last modified:April 3, 2007 - v1
Checksum:i8E23BD29391ABE4E
GO
Isoform 2 (identifier: A3KGF7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     55-77: Missing.
     689-702: IISGQFLSERSVRT → ARPRADKWLGGLGS
     703-1181: Missing.

Show »
Length:679
Mass (Da):77,235
Checksum:i671A64A042AE7024
GO
Isoform 3 (identifier: A3KGF7-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     689-702: IISGQFLSERSVRT → ARPRADKWLGGLGS
     703-1181: Missing.

Show »
Length:702
Mass (Da):79,946
Checksum:iB07FB842B2C8CC73
GO
Isoform 4 (identifier: A3KGF7-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.
     637-688: DLPMQQNMAL...VVVATTLSIT → GKNTSKTREP...DPYSSLDKEL
     689-1181: Missing.

Show »
Length:633
Mass (Da):71,354
Checksum:i30D14CB6B1901634
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5555Missing in isoform 4. VSP_026981Add
BLAST
Alternative sequencei55 – 7723Missing in isoform 2. VSP_026982Add
BLAST
Alternative sequencei637 – 68852DLPMQ…TLSIT → GKNTSKTREPSPGPLTTSPE PSPGPCIPRCPPSEPSSGPC LSDPYSSLDKEL in isoform 4. VSP_026983Add
BLAST
Alternative sequencei689 – 1181493Missing in isoform 4. VSP_026984Add
BLAST
Alternative sequencei689 – 70214IISGQ…RSVRT → ARPRADKWLGGLGS in isoform 2 and isoform 3. VSP_026985Add
BLAST
Alternative sequencei703 – 1181479Missing in isoform 2 and isoform 3. VSP_026986Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY902324 Genomic DNA. Translation: AAX90609.1.
AK045469 mRNA. Translation: BAC32384.1.
AK169442 mRNA. Translation: BAE41180.1.
AL772255 Genomic DNA. Translation: CAM45993.1.
AL772255 Genomic DNA. Translation: CAM45994.1.
AL772255 Genomic DNA. Translation: CAM45995.1.
CCDSiCCDS16582.1. [A3KGF7-1]
RefSeqiNP_001277719.1. NM_001290790.1.
NP_808236.2. NM_177568.2. [A3KGF7-1]
UniGeneiMm.215156.

Genome annotation databases

EnsembliENSMUST00000102524; ENSMUSP00000099583; ENSMUSG00000040061. [A3KGF7-1]
GeneIDi18796.
KEGGimmu:18796.
UCSCiuc008lsi.1. mouse. [A3KGF7-1]
uc008lsk.1. mouse. [A3KGF7-2]
uc008lsl.1. mouse. [A3KGF7-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY902324 Genomic DNA. Translation: AAX90609.1 .
AK045469 mRNA. Translation: BAC32384.1 .
AK169442 mRNA. Translation: BAE41180.1 .
AL772255 Genomic DNA. Translation: CAM45993.1 .
AL772255 Genomic DNA. Translation: CAM45994.1 .
AL772255 Genomic DNA. Translation: CAM45995.1 .
CCDSi CCDS16582.1. [A3KGF7-1 ]
RefSeqi NP_001277719.1. NM_001290790.1.
NP_808236.2. NM_177568.2. [A3KGF7-1 ]
UniGenei Mm.215156.

3D structure databases

ProteinModelPortali A3KGF7.
SMRi A3KGF7. Positions 11-834, 880-1147.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi A3KGF7. 6 interactions.

PTM databases

PhosphoSitei A3KGF7.

Proteomic databases

PaxDbi A3KGF7.
PRIDEi A3KGF7.

Protocols and materials databases

DNASUi 18796.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000102524 ; ENSMUSP00000099583 ; ENSMUSG00000040061 . [A3KGF7-1 ]
GeneIDi 18796.
KEGGi mmu:18796.
UCSCi uc008lsi.1. mouse. [A3KGF7-1 ]
uc008lsk.1. mouse. [A3KGF7-2 ]
uc008lsl.1. mouse. [A3KGF7-4 ]

Organism-specific databases

CTDi 5330.
MGIi MGI:107465. Plcb2.

Phylogenomic databases

eggNOGi NOG149692.
GeneTreei ENSGT00730000110266.
HOGENOMi HOG000232046.
HOVERGENi HBG053609.
InParanoidi A3KGF7.
KOi K05858.
OMAi FIKWDDE.
OrthoDBi EOG7WDN1N.
PhylomeDBi A3KGF7.
TreeFami TF313216.

Enzyme and pathway databases

Reactomei REACT_196473. Synthesis of IP3 and IP4 in the cytosol.
REACT_198612. Presynaptic function of Kainate receptors.
REACT_207651. G alpha (q) signalling events.
REACT_221970. Ca2+ pathway.

Miscellaneous databases

ChiTaRSi PLCB2. mouse.
NextBioi 295084.
PROi A3KGF7.
SOURCEi Search...

Gene expression databases

ArrayExpressi A3KGF7.
Bgeei A3KGF7.
CleanExi MM_PLCB2.
Genevestigatori A3KGF7.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028403. PLC-beta2.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF10. PTHR10336:SF10. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF000956. PLC-beta. 1 hit.
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of quantitative trait loci influencing growth and adiposity using congenic mouse strains."
    Farber C.R., Corva P.M., Medrano J.F.
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 3).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
    Strain: C57BL/6J.
    Tissue: Corpora quadrigemina and Thymus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: C57BL/6J.

Entry informationi

Entry nameiPLCB2_MOUSE
AccessioniPrimary (citable) accession number: A3KGF7
Secondary accession number(s): Q2M4J1, Q3TER8, Q8BI81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: April 3, 2007
Last modified: September 3, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The receptor-mediated activation of PLC-beta-2 is most effectively mediated by one G-protein alpha subunit, alpha-16 By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi