Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A3KGF7

- PLCB2_MOUSE

UniProt

A3KGF7 - PLCB2_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

Gene

Plcb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli (By similarity).By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Ca2+By similarityNote: Binds 1 Ca(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei327 – 3271PROSITE-ProRule annotation
Metal bindingi328 – 3281CalciumBy similarity
Metal bindingi357 – 3571CalciumBy similarity
Metal bindingi359 – 3591CalciumBy similarity
Active sitei374 – 3741PROSITE-ProRule annotation
Metal bindingi408 – 4081CalciumBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. lipid catabolic process Source: UniProtKB-KW
  3. sensory perception of bitter taste Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196473. Synthesis of IP3 and IP4 in the cytosol.
REACT_198612. Presynaptic function of Kainate receptors.
REACT_207651. G alpha (q) signalling events.
REACT_221970. Ca2+ pathway.
REACT_247495. Acetylcholine regulates insulin secretion.
REACT_253342. PLC beta mediated events.
REACT_258788. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
REACT_262889. G beta:gamma signalling through PLC beta.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-beta-2
Phospholipase C-beta-2
Short name:
PLC-beta-2
Gene namesi
Name:Plcb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:107465. Plcb2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 118111811-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2PRO_0000295683Add
BLAST

Proteomic databases

PaxDbiA3KGF7.
PRIDEiA3KGF7.

PTM databases

PhosphoSiteiA3KGF7.

Expressioni

Gene expression databases

BgeeiA3KGF7.
CleanExiMM_PLCB2.
ExpressionAtlasiA3KGF7. baseline and differential.
GenevestigatoriA3KGF7.

Interactioni

Subunit structurei

Interacts with RAC1.By similarity

Protein-protein interaction databases

IntActiA3KGF7. 6 interactions.

Structurei

3D structure databases

ProteinModelPortaliA3KGF7.
SMRiA3KGF7. Positions 11-834, 880-1147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini312 – 463152PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini547 – 663117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini670 – 76899C2PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili893 – 94048Sequence AnalysisAdd
BLAST
Coiled coili974 – 102653Sequence AnalysisAdd
BLAST
Coiled coili1075 – 114167Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi492 – 54655Glu-richAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000232046.
HOVERGENiHBG053609.
InParanoidiA3KGF7.
KOiK05858.
OMAiFIKWDDE.
OrthoDBiEOG7WDN1N.
PhylomeDBiA3KGF7.
TreeFamiTF313216.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028403. PLC-beta2.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF10. PTHR10336:SF10. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000956. PLC-beta. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: A3KGF7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLNPVLLP PNVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT
60 70 80 90 100
YQNQEMEFLD VTSIRDTRFG KFAKIPKSQK LREVFNMDFP DNHFLLKTLT
110 120 130 140 150
VVSGPDMVDL TFYNFVSYKE NVGKDWAEDV LALAKHPMTV NAPRSTFLDK
160 170 180 190 200
ILVKLKMQLN PEGKIPVKNF FQMFPADRKR VEAALGACHL AKGKNDAINP
210 220 230 240 250
EDFPESVYKS FLMSLCPRPE IDEIFTSYHS KAKPYMTKEH LTKFINQKQR
260 270 280 290 300
DPRLNSLLFP PARPEQVQVL IDKYEPSGIN VQRGQLSPEG MVWFLCGPEN
310 320 330 340 350
SVLAHDTLLI HQDMTQPLNH YFINSSHNTY LTAGQFSGLS SAEMYRQVLL
360 370 380 390 400
SGCRCVELDC WKGKPPDEEP IITHGFTMTT DILFKEAIEA IAESAFKTSP
410 420 430 440 450
YPVILSFENH VDSPRQQAKM AEYCRSMFGE TLLTDPLENF PLKPGIPLPS
460 470 480 490 500
PEDLRGKILI KNKKNQFSGP ASPSKKPGGV AEGSLPSSVP VEEDTGWTAE
510 520 530 540 550
DRTEVEEEEV VEEEEEEESG NLDEEEIKKM QSDEGTAGLE VTAYEEMSSL
560 570 580 590 600
VNYIQPTKFI SFEFSAQKNR SYVVSSFTEL KAYELLSKAS MQFVDYNKRQ
610 620 630 640 650
MSRVYPKGTR MDSSNYMPQM FWNAGCQMVA LNFQTMDLPM QQNMALFEFN
660 670 680 690 700
GQSGYLLKHE FMRRLDKQFN PFSVDRIDVV VATTLSITII SGQFLSERSV
710 720 730 740 750
RTYVEVELFG LPGDPKRRYR TKLSPTANSI NPVWKEEPFI FEKILMPELA
760 770 780 790 800
SLRIAVMEEG SKFLGHRIIP INALHSGYHH LCLRSESNMA LTMPALFVFL
810 820 830 840 850
EMKDYIPDTW ADLTVALANP IKYFNAQDKK SVKLKGVTGS LPEKLFSGTP
860 870 880 890 900
VASQSNGAPV SAGNGSTAPG TKATGEEATK EVTEPQTASL EELRELKGVV
910 920 930 940 950
KLQRRHEKEL RELERRGARR WEELLQRGAA QLAELQTQAA GCKLRPGKGS
960 970 980 990 1000
RKKRTLPCEE TVVAPSEPHD RADPRVQELK DRLEQELQQQ GEEQYRSVLK
1010 1020 1030 1040 1050
RKEQHVTEQI AKMMELAREK QAAELKTFKE TSETDTKEMK KKLEAKRLER
1060 1070 1080 1090 1100
IQAMTKVTTD KVAQERLKRE INNSHIQEVV QAVKQMTETL ERHQEKLEER
1110 1120 1130 1140 1150
QTACLEQIQA MEKQFQEKAL AEYEAKMKGL EAEVKESVRA YFKDCFPTEA
1160 1170 1180
EDKPERSCEA SEESCPQEPL VSKADTQESR L
Length:1,181
Mass (Da):134,546
Last modified:April 3, 2007 - v1
Checksum:i8E23BD29391ABE4E
GO
Isoform 2 (identifier: A3KGF7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     55-77: Missing.
     689-702: IISGQFLSERSVRT → ARPRADKWLGGLGS
     703-1181: Missing.

Show »
Length:679
Mass (Da):77,235
Checksum:i671A64A042AE7024
GO
Isoform 3 (identifier: A3KGF7-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     689-702: IISGQFLSERSVRT → ARPRADKWLGGLGS
     703-1181: Missing.

Show »
Length:702
Mass (Da):79,946
Checksum:iB07FB842B2C8CC73
GO
Isoform 4 (identifier: A3KGF7-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.
     637-688: DLPMQQNMAL...VVVATTLSIT → GKNTSKTREP...DPYSSLDKEL
     689-1181: Missing.

Show »
Length:633
Mass (Da):71,354
Checksum:i30D14CB6B1901634
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5555Missing in isoform 4. 1 PublicationVSP_026981Add
BLAST
Alternative sequencei55 – 7723Missing in isoform 2. 1 PublicationVSP_026982Add
BLAST
Alternative sequencei637 – 68852DLPMQ…TLSIT → GKNTSKTREPSPGPLTTSPE PSPGPCIPRCPPSEPSSGPC LSDPYSSLDKEL in isoform 4. 1 PublicationVSP_026983Add
BLAST
Alternative sequencei689 – 1181493Missing in isoform 4. 1 PublicationVSP_026984Add
BLAST
Alternative sequencei689 – 70214IISGQ…RSVRT → ARPRADKWLGGLGS in isoform 2 and isoform 3. 1 PublicationVSP_026985Add
BLAST
Alternative sequencei703 – 1181479Missing in isoform 2 and isoform 3. 1 PublicationVSP_026986Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY902324 Genomic DNA. Translation: AAX90609.1.
AK045469 mRNA. Translation: BAC32384.1.
AK169442 mRNA. Translation: BAE41180.1.
AL772255 Genomic DNA. Translation: CAM45993.1.
AL772255 Genomic DNA. Translation: CAM45994.1.
AL772255 Genomic DNA. Translation: CAM45995.1.
CCDSiCCDS16582.1. [A3KGF7-1]
RefSeqiNP_001277719.1. NM_001290790.1.
NP_808236.2. NM_177568.2. [A3KGF7-1]
UniGeneiMm.215156.

Genome annotation databases

EnsembliENSMUST00000102524; ENSMUSP00000099583; ENSMUSG00000040061. [A3KGF7-1]
GeneIDi18796.
KEGGimmu:18796.
UCSCiuc008lsi.1. mouse. [A3KGF7-1]
uc008lsk.1. mouse. [A3KGF7-2]
uc008lsl.1. mouse. [A3KGF7-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY902324 Genomic DNA. Translation: AAX90609.1 .
AK045469 mRNA. Translation: BAC32384.1 .
AK169442 mRNA. Translation: BAE41180.1 .
AL772255 Genomic DNA. Translation: CAM45993.1 .
AL772255 Genomic DNA. Translation: CAM45994.1 .
AL772255 Genomic DNA. Translation: CAM45995.1 .
CCDSi CCDS16582.1. [A3KGF7-1 ]
RefSeqi NP_001277719.1. NM_001290790.1.
NP_808236.2. NM_177568.2. [A3KGF7-1 ]
UniGenei Mm.215156.

3D structure databases

ProteinModelPortali A3KGF7.
SMRi A3KGF7. Positions 11-834, 880-1147.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi A3KGF7. 6 interactions.

PTM databases

PhosphoSitei A3KGF7.

Proteomic databases

PaxDbi A3KGF7.
PRIDEi A3KGF7.

Protocols and materials databases

DNASUi 18796.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000102524 ; ENSMUSP00000099583 ; ENSMUSG00000040061 . [A3KGF7-1 ]
GeneIDi 18796.
KEGGi mmu:18796.
UCSCi uc008lsi.1. mouse. [A3KGF7-1 ]
uc008lsk.1. mouse. [A3KGF7-2 ]
uc008lsl.1. mouse. [A3KGF7-4 ]

Organism-specific databases

CTDi 5330.
MGIi MGI:107465. Plcb2.

Phylogenomic databases

eggNOGi NOG149692.
GeneTreei ENSGT00760000118936.
HOGENOMi HOG000232046.
HOVERGENi HBG053609.
InParanoidi A3KGF7.
KOi K05858.
OMAi FIKWDDE.
OrthoDBi EOG7WDN1N.
PhylomeDBi A3KGF7.
TreeFami TF313216.

Enzyme and pathway databases

Reactomei REACT_196473. Synthesis of IP3 and IP4 in the cytosol.
REACT_198612. Presynaptic function of Kainate receptors.
REACT_207651. G alpha (q) signalling events.
REACT_221970. Ca2+ pathway.
REACT_247495. Acetylcholine regulates insulin secretion.
REACT_253342. PLC beta mediated events.
REACT_258788. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
REACT_262889. G beta:gamma signalling through PLC beta.

Miscellaneous databases

ChiTaRSi Plcb2. mouse.
NextBioi 295084.
PROi A3KGF7.
SOURCEi Search...

Gene expression databases

Bgeei A3KGF7.
CleanExi MM_PLCB2.
ExpressionAtlasi A3KGF7. baseline and differential.
Genevestigatori A3KGF7.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028403. PLC-beta2.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF10. PTHR10336:SF10. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF000956. PLC-beta. 1 hit.
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of quantitative trait loci influencing growth and adiposity using congenic mouse strains."
    Farber C.R., Corva P.M., Medrano J.F.
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 3).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
    Strain: C57BL/6J.
    Tissue: Corpora quadrigemina and Thymus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: C57BL/6J.

Entry informationi

Entry nameiPLCB2_MOUSE
AccessioniPrimary (citable) accession number: A3KGF7
Secondary accession number(s): Q2M4J1, Q3TER8, Q8BI81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: April 3, 2007
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The receptor-mediated activation of PLC-beta-2 is most effectively mediated by one G-protein alpha subunit, alpha-16.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3