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A3KGF7 (PLCB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase C-beta-2
Phospholipase C-beta-2
Short name=PLC-beta-2
Gene names
Name:Plcb2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1181 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli By similarity.

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subunit structure

Interacts with RAC1 By similarity.

Miscellaneous

The receptor-mediated activation of PLC-beta-2 is most effectively mediated by one G-protein alpha subunit, alpha-16 By similarity.

Sequence similarities

Contains 1 C2 domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A3KGF7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A3KGF7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     55-77: Missing.
     689-702: IISGQFLSERSVRT → ARPRADKWLGGLGS
     703-1181: Missing.
Isoform 3 (identifier: A3KGF7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     689-702: IISGQFLSERSVRT → ARPRADKWLGGLGS
     703-1181: Missing.
Isoform 4 (identifier: A3KGF7-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.
     637-688: DLPMQQNMAL...VVVATTLSIT → GKNTSKTREP...DPYSSLDKEL
     689-1181: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 118111811-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2
PRO_0000295683

Regions

Domain312 – 463152PI-PLC X-box
Domain547 – 663117PI-PLC Y-box
Domain670 – 76899C2
Coiled coil893 – 94048 Potential
Coiled coil974 – 102653 Potential
Coiled coil1075 – 114167 Potential
Compositional bias492 – 54655Glu-rich

Sites

Active site3271 By similarity
Active site3741 By similarity
Metal binding3281Calcium By similarity
Metal binding3571Calcium By similarity
Metal binding3591Calcium By similarity
Metal binding4081Calcium By similarity

Natural variations

Alternative sequence1 – 5555Missing in isoform 4.
VSP_026981
Alternative sequence55 – 7723Missing in isoform 2.
VSP_026982
Alternative sequence637 – 68852DLPMQ…TLSIT → GKNTSKTREPSPGPLTTSPE PSPGPCIPRCPPSEPSSGPC LSDPYSSLDKEL in isoform 4.
VSP_026983
Alternative sequence689 – 1181493Missing in isoform 4.
VSP_026984
Alternative sequence689 – 70214IISGQ…RSVRT → ARPRADKWLGGLGS in isoform 2 and isoform 3.
VSP_026985
Alternative sequence703 – 1181479Missing in isoform 2 and isoform 3.
VSP_026986

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: 8E23BD29391ABE4E

FASTA1,181134,546
        10         20         30         40         50         60 
MSLLNPVLLP PNVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT YQNQEMEFLD 

        70         80         90        100        110        120 
VTSIRDTRFG KFAKIPKSQK LREVFNMDFP DNHFLLKTLT VVSGPDMVDL TFYNFVSYKE 

       130        140        150        160        170        180 
NVGKDWAEDV LALAKHPMTV NAPRSTFLDK ILVKLKMQLN PEGKIPVKNF FQMFPADRKR 

       190        200        210        220        230        240 
VEAALGACHL AKGKNDAINP EDFPESVYKS FLMSLCPRPE IDEIFTSYHS KAKPYMTKEH 

       250        260        270        280        290        300 
LTKFINQKQR DPRLNSLLFP PARPEQVQVL IDKYEPSGIN VQRGQLSPEG MVWFLCGPEN 

       310        320        330        340        350        360 
SVLAHDTLLI HQDMTQPLNH YFINSSHNTY LTAGQFSGLS SAEMYRQVLL SGCRCVELDC 

       370        380        390        400        410        420 
WKGKPPDEEP IITHGFTMTT DILFKEAIEA IAESAFKTSP YPVILSFENH VDSPRQQAKM 

       430        440        450        460        470        480 
AEYCRSMFGE TLLTDPLENF PLKPGIPLPS PEDLRGKILI KNKKNQFSGP ASPSKKPGGV 

       490        500        510        520        530        540 
AEGSLPSSVP VEEDTGWTAE DRTEVEEEEV VEEEEEEESG NLDEEEIKKM QSDEGTAGLE 

       550        560        570        580        590        600 
VTAYEEMSSL VNYIQPTKFI SFEFSAQKNR SYVVSSFTEL KAYELLSKAS MQFVDYNKRQ 

       610        620        630        640        650        660 
MSRVYPKGTR MDSSNYMPQM FWNAGCQMVA LNFQTMDLPM QQNMALFEFN GQSGYLLKHE 

       670        680        690        700        710        720 
FMRRLDKQFN PFSVDRIDVV VATTLSITII SGQFLSERSV RTYVEVELFG LPGDPKRRYR 

       730        740        750        760        770        780 
TKLSPTANSI NPVWKEEPFI FEKILMPELA SLRIAVMEEG SKFLGHRIIP INALHSGYHH 

       790        800        810        820        830        840 
LCLRSESNMA LTMPALFVFL EMKDYIPDTW ADLTVALANP IKYFNAQDKK SVKLKGVTGS 

       850        860        870        880        890        900 
LPEKLFSGTP VASQSNGAPV SAGNGSTAPG TKATGEEATK EVTEPQTASL EELRELKGVV 

       910        920        930        940        950        960 
KLQRRHEKEL RELERRGARR WEELLQRGAA QLAELQTQAA GCKLRPGKGS RKKRTLPCEE 

       970        980        990       1000       1010       1020 
TVVAPSEPHD RADPRVQELK DRLEQELQQQ GEEQYRSVLK RKEQHVTEQI AKMMELAREK 

      1030       1040       1050       1060       1070       1080 
QAAELKTFKE TSETDTKEMK KKLEAKRLER IQAMTKVTTD KVAQERLKRE INNSHIQEVV 

      1090       1100       1110       1120       1130       1140 
QAVKQMTETL ERHQEKLEER QTACLEQIQA MEKQFQEKAL AEYEAKMKGL EAEVKESVRA 

      1150       1160       1170       1180 
YFKDCFPTEA EDKPERSCEA SEESCPQEPL VSKADTQESR L 

« Hide

Isoform 2 [UniParc].

Checksum: 671A64A042AE7024
Show »

FASTA67977,235
Isoform 3 [UniParc].

Checksum: B07FB842B2C8CC73
Show »

FASTA70279,946
Isoform 4 [UniParc].

Checksum: 30D14CB6B1901634
Show »

FASTA63371,354

References

« Hide 'large scale' references
[1]"Characterization of quantitative trait loci influencing growth and adiposity using congenic mouse strains."
Farber C.R., Corva P.M., Medrano J.F.
Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 3).
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
Strain: C57BL/6J.
Tissue: Corpora quadrigemina and Thymus.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY902324 Genomic DNA. Translation: AAX90609.1.
AK045469 mRNA. Translation: BAC32384.1.
AK169442 mRNA. Translation: BAE41180.1.
AL772255 Genomic DNA. Translation: CAM45993.1.
AL772255 Genomic DNA. Translation: CAM45994.1.
AL772255 Genomic DNA. Translation: CAM45995.1.
CCDSCCDS16582.1. [A3KGF7-1]
RefSeqNP_001277719.1. NM_001290790.1.
NP_808236.2. NM_177568.2. [A3KGF7-1]
UniGeneMm.215156.

3D structure databases

ProteinModelPortalA3KGF7.
SMRA3KGF7. Positions 11-834, 880-1147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActA3KGF7. 6 interactions.

PTM databases

PhosphoSiteA3KGF7.

Proteomic databases

PaxDbA3KGF7.
PRIDEA3KGF7.

Protocols and materials databases

DNASU18796.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102524; ENSMUSP00000099583; ENSMUSG00000040061. [A3KGF7-1]
GeneID18796.
KEGGmmu:18796.
UCSCuc008lsi.1. mouse. [A3KGF7-1]
uc008lsk.1. mouse. [A3KGF7-2]
uc008lsl.1. mouse. [A3KGF7-4]

Organism-specific databases

CTD5330.
MGIMGI:107465. Plcb2.

Phylogenomic databases

eggNOGNOG149692.
GeneTreeENSGT00730000110266.
HOGENOMHOG000232046.
HOVERGENHBG053609.
InParanoidA3KGF7.
KOK05858.
OMAFIKWDDE.
OrthoDBEOG7WDN1N.
PhylomeDBA3KGF7.
TreeFamTF313216.

Gene expression databases

ArrayExpressA3KGF7.
BgeeA3KGF7.
CleanExMM_PLCB2.
GenevestigatorA3KGF7.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028403. PLC-beta2.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF10. PTHR10336:SF10. 1 hit.
PfamPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFPIRSF000956. PLC-beta. 1 hit.
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPLCB2. mouse.
NextBio295084.
PROA3KGF7.
SOURCESearch...

Entry information

Entry namePLCB2_MOUSE
AccessionPrimary (citable) accession number: A3KGF7
Secondary accession number(s): Q2M4J1, Q3TER8, Q8BI81
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: April 3, 2007
Last modified: July 9, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot