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Protein

Chromodomain-helicase-DNA-binding protein 6

Gene

Chd6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable chromatin-remodeling protein with a DNA-dependent ATPase activity. May play a role in transcription regulation, activating for instance, the transcription of specific genes in response to oxidative stress through interaction with NFE2L2 (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi485 – 4928ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Helicase, Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chromodomain-helicase-DNA-binding protein 6 (EC:3.6.4.12)
Short name:
CHD-6
Alternative name(s):
ATP-dependent helicase CHD6
Gene namesi
Name:Chd6
Synonyms:Kiaa1335
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1918639. Chd6.

Subcellular locationi

  • Nucleusnucleoplasm By similarity

  • Note: Enriched at sites of mRNA synthesis.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice with targeted deletion of exon 12 lacking part of the Helicase ATP-binding domain are born in normal Mendelian ratios and are viable. They are fertile and exhibit no obvious morphological or histological difference. However, they display a coordination deficiency which is not due to muscle weakness or bradykinesia.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 27112711Chromodomain-helicase-DNA-binding protein 6PRO_0000429353Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1865 – 18651PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA3KFM7.
PaxDbiA3KFM7.
PeptideAtlasiA3KFM7.
PRIDEiA3KFM7.

PTM databases

iPTMnetiA3KFM7.
PhosphoSiteiA3KFM7.

Expressioni

Tissue specificityi

Expressed in all tissues tested including liver, heart, kidney, spleen and lung (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000057133.
ExpressionAtlasiA3KFM7. baseline and differential.
GenevisibleiA3KFM7. MM.

Interactioni

Subunit structurei

Interacts with NFE2L2; involved in activation of the transcription. May interact with PPARA (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiA3KFM7. 1 interaction.
STRINGi10090.ENSMUSP00000042291.

Structurei

3D structure databases

ProteinModelPortaliA3KFM7.
SMRiA3KFM7. Positions 370-430.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini291 – 34252Chromo 1PROSITE-ProRule annotationAdd
BLAST
Domaini374 – 43865Chromo 2PROSITE-ProRule annotationAdd
BLAST
Domaini472 – 646175Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini786 – 955170Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini1448 – 150255Myb-likeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 746746Required for DNA-dependent ATPase activityBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi597 – 6004DEAH box

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 2 chromo domains.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 Myb-like domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0383. Eukaryota.
COG0553. LUCA.
GeneTreeiENSGT00760000119067.
HOGENOMiHOG000246942.
HOVERGENiHBG081150.
InParanoidiA3KFM7.
KOiK14436.
OMAiTHGRFKW.
OrthoDBiEOG091G0022.
PhylomeDBiA3KFM7.
TreeFamiTF313572.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR006576. BRK_domain.
IPR000953. Chromo/shadow_dom.
IPR023780. Chromo_domain.
IPR016197. Chromodomain-like.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00385. Chromo. 2 hits.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00592. BRK. 1 hit.
SM00298. CHROMO. 2 hits.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF54160. SSF54160. 2 hits.
PROSITEiPS50013. CHROMO_2. 1 hit.
PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A3KFM7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKMKIQKKEK QLSKLRALNH SPMSDASVNF DYKSPSPFDC SPDQGENIEE
60 70 80 90 100
AANHCLPHKN LYTTEEEADT LFSRKLTSHN GMEDSGGRGT GVKKKRKKKE
110 120 130 140 150
PGEQEGTKGS KDREPKPKRK REPKEPKEPR RAKEPKRAKE PKETKQKDGV
160 170 180 190 200
KKPRKHREAS GTKEGKEKRS CTDYGSRTKS KKASREQGPT PVERKKKGKR
210 220 230 240 250
KNETTVESLE LDHSLPNPSL QSPEEPSESA DSQKRRSGRQ VKRRKYNEDL
260 270 280 290 300
DFKVVDDDGE TIAVLGAGRT SALSASTLAW QAEEPPEDDA NIIEKILASK
310 320 330 340 350
TVQEVHPGEP PFDLELFYVK YRNFSYLHCK WATMEELEKD PRIAQKIKRF
360 370 380 390 400
RNKQAQMKHI FTEPDEDLFN PDYIEIDRIL EVAHTKDAET GEEVTHYLVK
410 420 430 440 450
WCSLPYEEST WELEEDVDPA KVKEFESLQI LPEVKHVERP ASDAWQKLET
460 470 480 490 500
SREYRNSNRL REYQLEGMNW LLFNWYNRKN CILADEMGLG KTIQSIAFLS
510 520 530 540 550
EIFVRGIHGP FLIIAPLSTI TNWEREFRTW TEMNAIVYHG SQISRQMIQQ
560 570 580 590 600
YEMVYRDAQG NPLSGVFKFH VVITTFEMIL ADCPELKKIH WSCVIIDEAH
610 620 630 640 650
RLKNRNCKLL EGLKLMALEH KVLLTGTPLQ NSVEELFSLL NFLEPSQFPS
660 670 680 690 700
ETAFLEEFGD LKTEEQVKKL QSILKPMMLR RLKDDVEKNL APKQETIIEV
710 720 730 740 750
ELTNIQKKYY RAILEKNFSF LTKGANQHNM PNLINTMMEL RKCCNHPYLI
760 770 780 790 800
NGAEEKILED FRKAHSSEAS DFQLQAMIQA AGKLVLIDKL LPKLIAGGHK
810 820 830 840 850
VLIFSQMVRC LDILEDYLIQ RRYTYERIDG RVRGNLRQAA IDRFCKPDSD
860 870 880 890 900
RFVFLLCTRA GGLGINLTAA DTCIIFDSDW NPQNDLQAQA RCHRIGQSKA
910 920 930 940 950
VKVYRLITRN SYEREMFDKA SLKLGLDKAI LQDINRKGST NGVQQLSKME
960 970 980 990 1000
VEDLLRKGAY GALMDEEDEG SKFCEEDIDQ ILQRRTHTIT IQSEGKGSTF
1010 1020 1030 1040 1050
AKASFVASGN RTDISLDDPN FWQKWAKIAE LDTEANNEKE SLVIDRPRVR
1060 1070 1080 1090 1100
KQTKHYNSFE EDELMEFSEL DSDSDERPTR SRRLSDRARR YLRAECFRVE
1110 1120 1130 1140 1150
KNLLIFGWGR WKDILTHGRF KWPLNEKDME MICRALLVYC VKHYKGDEKI
1160 1170 1180 1190 1200
KSFIWELITP SKDGQVQTLQ NHSGLSAPVP RGRKGKKTKN QLLLPELKNA
1210 1220 1230 1240 1250
DWLATCNPEV VLHDDGYKKH LKQHCNKVLL RVRMLYYLKA EILGEAADKA
1260 1270 1280 1290 1300
FEGTPARELD VLLPDIDYVE IPVDWWDAEA DKSLLIGVFK HGYERYNAMR
1310 1320 1330 1340 1350
ADPALCFLEK VGMPDEKSLS AEQGVTDGTS DIPERGNIDK EDSAEDKLDG
1360 1370 1380 1390 1400
LQKQTASPSD GSDGIFGEKK DDSQAAQDGS DPDKSPWPVS SALTARLRRL
1410 1420 1430 1440 1450
VTIYQRCNRK ELCRPEILGP GNQGYWVQEE VFRRTSEMDL INKEAQKRWT
1460 1470 1480 1490 1500
RREQADFYRT VSSFGVVYDQ EKKAFDWTQF RIISRLDKKS DESLEHYFYS
1510 1520 1530 1540 1550
FVAMCRNVCR LPAWKDDGPP DASIYVEPIT EERAAKTLYR IELLRKVREQ
1560 1570 1580 1590 1600
VLMCPQLHER LQLCRPSLYL PVWWECGKHD RDLLIGTAKH GLNRTDYYIM
1610 1620 1630 1640 1650
NDPQLSFLDA YRNYAQHKRT DTQAPGSLCC LYQSNSKLYE SLTYTPVSRT
1660 1670 1680 1690 1700
SESLESEPEN LMRMESRDDH LCLPEGGLPD ITCENFVSKV QEVISLDHDE
1710 1720 1730 1740 1750
NLLPESLENM IYGKTGLSQE PHSFQEAPTT NTQSRKNTIT ISASRNESCQ
1760 1770 1780 1790 1800
PPGIEAEITS ASSLMSSLEA GVAKMNIKNG KHLLVSISKE GEPCCSETGR
1810 1820 1830 1840 1850
RPETIGHREA KCLVSPTLDT GHESGFVDLC SLSVYDPKRN FSSDQQLIDL
1860 1870 1880 1890 1900
LENKSLESKL ILNQSDEEEE ENEDETLAIV ASATEKPEVL DFPKPTVNIP
1910 1920 1930 1940 1950
RGKNLSFHQD EAKKGRLEVV SKTAGPQRVF PPPANQCHCK HIERWAHGLG
1960 1970 1980 1990 2000
SEDSEVEKPK AYQPDLYRSK ANNSTVEGET AVIPTEPFKL KHELLKEPWK
2010 2020 2030 2040 2050
ESSEGGKSFS MYAPEGSEPK PEDMDFENKD DYEKDGTCLS QDYPGKYSEE
2060 2070 2080 2090 2100
ESKSSASGIA GDLGEEAQEV RAPTIAQLLQ EKTLYSFSEW PKDRVIINRL
2110 2120 2130 2140 2150
DNICHVVLKG KWPCSHQYEP SGALPTPVLS SSAGSRSSLS EPEATEHGFS
2160 2170 2180 2190 2200
NGAALAAQIQ KESFLAPVFT KDEQKHRRPY EFEVERDAKA RSLEEYSATH
2210 2220 2230 2240 2250
GRPPIVLNGW HGESAIDLSC SSEGSPGATS PFPVSASTPK IGAISSLQGA
2260 2270 2280 2290 2300
LGMDLSGILQ AGLIHPVTGQ IVNGSLRRDD AAMRRRRGRR KHIEGGMDLI
2310 2320 2330 2340 2350
FLKEQTLQAG ILEVHEDAGQ TTLSTTHPEV PGATSSAPEP TAAASSQAEK
2360 2370 2380 2390 2400
AVPSKSLLDW LRQQADYSLD VPGFGTSFSD KPKQRRPRCK EPGKLDISSL
2410 2420 2430 2440 2450
GGEERVPAVP KEPGLRGFLP ESKFNHTLAE PVLRDAGPRR RGRRPRNELL
2460 2470 2480 2490 2500
KAPAIVADSP SGMGPLFMNG LIAGMDLVGL QNVRNIPGIP LTGLVGFPAG
2510 2520 2530 2540 2550
FATMPTGEEV KNTLSMLPMM LPGMAAVPQM FGVGGLLNTP MATTCTTTAS
2560 2570 2580 2590 2600
ASLASTKSGT SATEKSTEDK LSGHDVNTDA LVDDKPGPSP FSDQSEPTIT
2610 2620 2630 2640 2650
TSSPVAFNPF LIPGVSPGLI YPSMFLSPGM GMALPAMQQA RHSEMVGLET
2660 2670 2680 2690 2700
QKRKKKKTKG DSPTQEPASV CEKEPGSDQN CTESSATVSP EREHVAQARE
2710
EGLKDSNEDT N
Length:2,711
Mass (Da):305,397
Last modified:April 3, 2007 - v1
Checksum:i256A4983B8EA3544
GO
Isoform 2 (identifier: A3KFM7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     184-184: Missing.

Note: No experimental confirmation available.
Show »
Length:2,710
Mass (Da):305,310
Checksum:iADB0ED856383FDD9
GO

Sequence cautioni

The sequence BAC65778 differs from that shown.Probable cloning artifact. Spurious priming from an intronic poly-A tract.Curated
The sequence BAC65778 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti756 – 7561K → R in BAE24963 (PubMed:16141072).Curated
Sequence conflicti1305 – 13051L → H in BAE24963 (PubMed:16141072).Curated
Sequence conflicti1741 – 17411I → V in AAI50807 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei184 – 1841Missing in isoform 2. 1 PublicationVSP_054901

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590430 Genomic DNA. No translation available.
AL591763 Genomic DNA. No translation available.
BC150806 mRNA. Translation: AAI50807.1.
AK142177 mRNA. Translation: BAE24963.1.
AK122496 mRNA. Translation: BAC65778.2. Sequence problems.
CCDSiCCDS17000.1. [A3KFM7-1]
RefSeqiNP_775544.2. NM_173368.3. [A3KFM7-1]
XP_006500244.1. XM_006500181.2. [A3KFM7-2]
XP_011238078.1. XM_011239776.1. [A3KFM7-2]
UniGeneiMm.122738.

Genome annotation databases

EnsembliENSMUST00000039782; ENSMUSP00000042291; ENSMUSG00000057133. [A3KFM7-1]
GeneIDi71389.
KEGGimmu:71389.
UCSCiuc008nrn.2. mouse. [A3KFM7-1]
uc008nrp.2. mouse. [A3KFM7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590430 Genomic DNA. No translation available.
AL591763 Genomic DNA. No translation available.
BC150806 mRNA. Translation: AAI50807.1.
AK142177 mRNA. Translation: BAE24963.1.
AK122496 mRNA. Translation: BAC65778.2. Sequence problems.
CCDSiCCDS17000.1. [A3KFM7-1]
RefSeqiNP_775544.2. NM_173368.3. [A3KFM7-1]
XP_006500244.1. XM_006500181.2. [A3KFM7-2]
XP_011238078.1. XM_011239776.1. [A3KFM7-2]
UniGeneiMm.122738.

3D structure databases

ProteinModelPortaliA3KFM7.
SMRiA3KFM7. Positions 370-430.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiA3KFM7. 1 interaction.
STRINGi10090.ENSMUSP00000042291.

PTM databases

iPTMnetiA3KFM7.
PhosphoSiteiA3KFM7.

Proteomic databases

MaxQBiA3KFM7.
PaxDbiA3KFM7.
PeptideAtlasiA3KFM7.
PRIDEiA3KFM7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039782; ENSMUSP00000042291; ENSMUSG00000057133. [A3KFM7-1]
GeneIDi71389.
KEGGimmu:71389.
UCSCiuc008nrn.2. mouse. [A3KFM7-1]
uc008nrp.2. mouse. [A3KFM7-2]

Organism-specific databases

CTDi84181.
MGIiMGI:1918639. Chd6.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0383. Eukaryota.
COG0553. LUCA.
GeneTreeiENSGT00760000119067.
HOGENOMiHOG000246942.
HOVERGENiHBG081150.
InParanoidiA3KFM7.
KOiK14436.
OMAiTHGRFKW.
OrthoDBiEOG091G0022.
PhylomeDBiA3KFM7.
TreeFamiTF313572.

Miscellaneous databases

ChiTaRSiChd6. mouse.
PROiA3KFM7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057133.
ExpressionAtlasiA3KFM7. baseline and differential.
GenevisibleiA3KFM7. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR006576. BRK_domain.
IPR000953. Chromo/shadow_dom.
IPR023780. Chromo_domain.
IPR016197. Chromodomain-like.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00385. Chromo. 2 hits.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00592. BRK. 1 hit.
SM00298. CHROMO. 2 hits.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF54160. SSF54160. 2 hits.
PROSITEiPS50013. CHROMO_2. 1 hit.
PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHD6_MOUSE
AccessioniPrimary (citable) accession number: A3KFM7
Secondary accession number(s): B9EKA7, Q3UQS6, Q80TE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 11, 2014
Last sequence update: April 3, 2007
Last modified: September 7, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.