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Protein

NADH-cytochrome b5 reductase 1

Gene

CBR1

Organism
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes (By similarity).By similarity

Catalytic activityi

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactori

FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi124 – 13916FADBy similarityAdd
BLAST
Nucleotide bindingi150 – 18233FADBy similarityAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-cytochrome b5 reductase 1 (EC:1.6.2.2)
Alternative name(s):
Microsomal cytochrome b reductase
Gene namesi
Name:CBR1
ORF Names:PICST_68997
OrganismiScheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
Taxonomic identifieri322104 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeScheffersomyces
Proteomesi
  • UP000002258 Componentsi: Chromosome 1, Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284NADH-cytochrome b5 reductase 1PRO_0000330163Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi322104.XP_001387732.2.

Structurei

3D structure databases

ProteinModelPortaliA3GF86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 144104FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
COG0543. LUCA.
HOGENOMiHOG000175005.
InParanoidiA3GF86.
KOiK00326.
OMAiAPMYQII.
OrthoDBiEOG092C43G8.

Family and domain databases

InterProiIPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR001834. NADH-Cyt_B5_reductase.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00406. CYTB5RDTASE.
PR00371. FPNCR.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3GF86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADTEPSPLF VFSTIAIIIS TFVIFYFVQQ SKKNTPVLKP DTFQKFPLIE
60 70 80 90 100
KTRVSHNSSV YRFGLPKSTD RLGLPIGQHI SIGATIGGKE VVRSYTPIST
110 120 130 140 150
DDELGYFDLL IKTYENGNIS KHVDSKKVGE YVEIRGPKGF FTYTPNMVKS
160 170 180 190 200
FGMIAGGTGI APMYQIITAI LRNPADKTKI SLIYANVTES DILLKSELDK
210 220 230 240 250
WAEEHPDNFS VHYVLNEAPE NWKGSVGFVT PEIIDSKLPK ASDDSNLLLC
260 270 280
GPPPMISAMK KAAVGLGFAK AKPVSKLGDQ VFVF
Length:284
Mass (Da):31,274
Last modified:January 19, 2010 - v2
Checksum:i0B825044D2C04489
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAVQ01000001 Genomic DNA. Translation: EAZ63709.2.
RefSeqiXP_001387732.2. XM_001387695.1.

Genome annotation databases

EnsemblFungiiEAZ63709; EAZ63709; PICST_68997.
GeneIDi4850935.
KEGGipic:PICST_68997.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAVQ01000001 Genomic DNA. Translation: EAZ63709.2.
RefSeqiXP_001387732.2. XM_001387695.1.

3D structure databases

ProteinModelPortaliA3GF86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi322104.XP_001387732.2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAZ63709; EAZ63709; PICST_68997.
GeneIDi4850935.
KEGGipic:PICST_68997.

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
COG0543. LUCA.
HOGENOMiHOG000175005.
InParanoidiA3GF86.
KOiK00326.
OMAiAPMYQII.
OrthoDBiEOG092C43G8.

Family and domain databases

InterProiIPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR001834. NADH-Cyt_B5_reductase.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00406. CYTB5RDTASE.
PR00371. FPNCR.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCB5R_PICST
AccessioniPrimary (citable) accession number: A3GF86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: January 19, 2010
Last modified: September 7, 2016
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.