Reviewed, UniProtKB/Swiss-Prot A3GF86 (NCB5R_PICST)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 1
    EC=1.6.2.2
Alternative name(s):
    Microsomal cytochrome b reductase

Gene names

Name:	CBR1
ORF Names:	PICST_68997

Organism

Pichia stipitis (Yeast) [Complete proteome]

Taxonomic identifier

4924 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	NADH-cytochrome b5 reductase 1		PRO_0000330163
Regions
	Transmembrane	8 – 28	21	Potential
	Domain	41 – 144	104	FAD-binding FR-type
	Nucleotide binding	124 – 139	16	FAD By similarity
	Nucleotide binding	150 – 182	33	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3GF86-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 0DD0BF44DC0F9489	FASTA	284	31,300
	10 20 30 40 50 60 MADTEPSPLF VFSTIAIIIL TFVIFYFVQQ SKKNTPVLKP DTFQKFPLIE KTRVSHNSSV 70 80 90 100 110 120 YRFGLPKSTD RLGLPIGQHI SIGATIGGKE VVRSYTPIST DDELGYFDLL IKTYENGNIS 130 140 150 160 170 180 KHVDSKKVGE YVEIRGPKGF FTYTPNMVKS FGMIAGGTGI APMYQIITAI LRNPADKTKI 190 200 210 220 230 240 SLIYANVTES DILLKSELDK WAEEHPDNFS VHYVLNEAPE NWKGSVGFVT PEIIDSKLPK 250 260 270 280 ASDDSNLLLC GPPPMISAMK KAAVGLGFAK AKPVSKLGDQ VFVF

References

[1]

"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed: 17334359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAVQ01000001 Genomic DNA. Translation: EAZ63709.1.
RefSeq	XP_001387732.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3GF86.
Genome annotation databases
GeneID	4850935.
GenomeReviews	Gene locus CBR1 in contig CM000437_GR.
Phylogenomic databases
OMA	ARFKFAL.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCB5R_PICST

Accession

Primary (citable) accession number: A3GF86

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)