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Protein

Glycerol-3-phosphate acyltransferase 4

Gene

GPAT4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Active against both saturated and unsaturated long-chain fatty acyl-CoAs (By similarity).By similarity

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 4 (GPAT4), Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (AGPAT1), 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (AGPAT4)
  3. Phosphatidate cytidylyltransferase, mitochondrial (TAMM41), Phosphatidate cytidylyltransferase 2 (CDS2), Phosphatidate cytidylyltransferase, mitochondrial (TAMM41), Phosphatidate cytidylyltransferase (CDS1)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate acyltransferase 4By similarity (EC:2.3.1.15)
Short name:
GPAT4
Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 6
Short name:
1-AGP acyltransferase 6
Short name:
1-AGPAT 6
Acyl-CoA:glycerol-3-phosphate acyltransferase 4
Lysophosphatidic acid acyltransferase zeta
Short name:
LPAAT-zeta
Gene namesi
Name:GPAT4By similarity
Synonyms:AGPAT6
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei156 – 176HelicalSequence analysisAdd BLAST21
Transmembranei180 – 200HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Sequence analysisAdd BLAST37
ChainiPRO_000029748838 – 456Glycerol-3-phosphate acyltransferase 4Add BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi247N-linked (GlcNAc...)Sequence analysis1
Glycosylationi327N-linked (GlcNAc...)Sequence analysis1
Glycosylationi328N-linked (GlcNAc...)Sequence analysis1
Glycosylationi362N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiA3FPG8.
PRIDEiA3FPG8.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007532.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi248 – 253HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2898. Eukaryota.
COG0204. LUCA.
HOGENOMiHOG000265725.
InParanoidiA3FPG8.
KOiK13506.

Family and domain databases

InterProiIPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A3FPG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLLLPFDSL IVSLLGISLT VLFTLLLVFI IVPAVFGVSF GIRKLYMKTL
60 70 80 90 100
LKIFAWATLR MERGAKEKNH QLYKPYTNGI IAKDPTSLEE EIKEIRRSGS
110 120 130 140 150
SKALDNTPEF ELSDIFYFCR KGMETIMDDE VTKRFSAEEL ESWNLLSRTN
160 170 180 190 200
YNFQYISLRL TVLWGLGVLI RYCLLLSLRI ALAFTGISLL VVGTTMVGYL
210 220 230 240 250
PNGRFKEFLS KHVHLMCYRI CVRALTAIIT YHDRKNRPRN GGICVANHTS
260 270 280 290 300
PIDVIILASD GYYAMVGQVH GGLMGVIQRA MVKACPHVWF ERSEVKDRHL
310 320 330 340 350
VARRLTEHVQ DKSKLPILIF PEGTCINNTS VMMFKKGSFE IGATVYPVAI
360 370 380 390 400
KYDPQFGDAF WNSSKYGMVT YLLRMMTSWA IVCSVWYLPP MTRQAEEDAV
410 420 430 440 450
QFANRVKSAI ARQGGLVDLL WDGGLKREKV KDTFKEEQQK LYSKMIVGNH

EDRSRS
Length:456
Mass (Da):52,055
Last modified:March 20, 2007 - v1
Checksum:i0102A42ADD0F058C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF432784 Genomic DNA. Translation: ABN50363.1.
RefSeqiNP_001077138.1. NM_001083669.1.
UniGeneiBt.16393.

Genome annotation databases

GeneIDi511614.
KEGGibta:511614.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF432784 Genomic DNA. Translation: ABN50363.1.
RefSeqiNP_001077138.1. NM_001083669.1.
UniGeneiBt.16393.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007532.

Proteomic databases

PaxDbiA3FPG8.
PRIDEiA3FPG8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi511614.
KEGGibta:511614.

Organism-specific databases

CTDi137964.

Phylogenomic databases

eggNOGiKOG2898. Eukaryota.
COG0204. LUCA.
HOGENOMiHOG000265725.
InParanoidiA3FPG8.
KOiK13506.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612.

Family and domain databases

InterProiIPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPAT4_BOVIN
AccessioniPrimary (citable) accession number: A3FPG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: March 20, 2007
Last modified: June 8, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.