Reviewed, UniProtKB/Swiss-Prot A3FPG8 (GPAT4_BOVIN)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase 4
      Short name=GPAT4
    EC=2.3.1.15
Alternative name(s):
    Acyl-CoA:glycerol-3-phosphate acyltransferase 4
    1-acylglycerol-3-phosphate O-acyltransferase 6
      Short name=1-AGP acyltransferase 6
      Short name=1-AGPAT 6
    Lysophosphatidic acid acyltransferase zeta
      Short name=LPAAT-zeta

Gene names

Name:	AGPAT6
Synonyms:	GPAT4

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Active against both saturated and unsaturated long-chain fatty acyl-CoAs By similarity.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane
Molecular function	Acyltransferase Transferase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 37	37	Potential
	Chain	38 – 456	419	Glycerol-3-phosphate acyltransferase 4		PRO_0000297488
Regions
	Transmembrane	156 – 176	21	Potential
	Transmembrane	180 – 200	21	Potential
	Motif	248 – 253	6	HXXXXD motif
Amino acid modifications
	Modified residue	218	1	Phosphotyrosine By similarity
	Glycosylation	247	1	N-linked (GlcNAc...) Potential
	Glycosylation	327	1	N-linked (GlcNAc...) Potential
	Glycosylation	328	1	N-linked (GlcNAc...) Potential
	Glycosylation	362	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A3FPG8-1 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 0102A42ADD0F058C	FASTA	456	52,055
	10 20 30 40 50 60 MFLLLPFDSL IVSLLGISLT VLFTLLLVFI IVPAVFGVSF GIRKLYMKTL LKIFAWATLR 70 80 90 100 110 120 MERGAKEKNH QLYKPYTNGI IAKDPTSLEE EIKEIRRSGS SKALDNTPEF ELSDIFYFCR 130 140 150 160 170 180 KGMETIMDDE VTKRFSAEEL ESWNLLSRTN YNFQYISLRL TVLWGLGVLI RYCLLLSLRI 190 200 210 220 230 240 ALAFTGISLL VVGTTMVGYL PNGRFKEFLS KHVHLMCYRI CVRALTAIIT YHDRKNRPRN 250 260 270 280 290 300 GGICVANHTS PIDVIILASD GYYAMVGQVH GGLMGVIQRA MVKACPHVWF ERSEVKDRHL 310 320 330 340 350 360 VARRLTEHVQ DKSKLPILIF PEGTCINNTS VMMFKKGSFE IGATVYPVAI KYDPQFGDAF 370 380 390 400 410 420 WNSSKYGMVT YLLRMMTSWA IVCSVWYLPP MTRQAEEDAV QFANRVKSAI ARQGGLVDLL 430 440 450 WDGGLKREKV KDTFKEEQQK LYSKMIVGNH EDRSRS

References

[1]	"Bos taurus 1-acylglycerol-3-phosphate O-acyltransferase 6 (AGPAT6) gene." Zhang J.L., Zan L.S. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	EF432784 Genomic DNA. Translation: ABN50363.1.
IPI	IPI00697212.
RefSeq	NP_001077138.1.
UniGene	Bt.16393
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3FPG8.
Genome annotation databases
Ensembl	ENSBTAT00000007532; ENSBTAP00000007532; ENSBTAG00000005730; Bos taurus. [Genome view]
GeneID	511614.
KEGG	bta:511614.
Organism-specific databases
CTD	511614.
Phylogenomic databases
HOVERGEN	A3FPG8.
Enzyme and pathway databases
BRENDA	2.3.1.51. 251.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPAT4_BOVIN

Accession

Primary (citable) accession number: A3FPG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	March 20, 2007
Last modified:	October 13, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)