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Protein

Phospholipid-transporting ATPase FetA

Gene

Atp8b5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

P4-ATPase flippase which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. May play a role in phospholid transport across membranes and in acrosome formation.1 Publication

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4164-aspartylphosphate intermediateBy similarity1
Metal bindingi838MagnesiumBy similarity1
Metal bindingi842MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • phospholipid-translocating ATPase activity Source: GO_Central
  • phospholipid transporter activity Source: UniProtKB

GO - Biological processi

  • Golgi organization Source: UniProtKB
  • phospholipid transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid-transporting ATPase FetA (EC:3.6.3.1)
Alternative name(s):
ATPase class I type 8B member 2-like protein
ATPase class I type 8B member 5
Flippase expressed in testis A
Gene namesi
Name:Atp8b5
Synonyms:Feta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2444287. Atp8b5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Transmembranei299 – 319HelicalSequence analysisAdd BLAST21
Transmembranei348 – 368HelicalSequence analysisAdd BLAST21
Transmembranei904 – 924HelicalSequence analysisAdd BLAST21
Transmembranei927 – 947HelicalSequence analysisAdd BLAST21
Transmembranei981 – 1001HelicalSequence analysisAdd BLAST21
Transmembranei1014 – 1034HelicalSequence analysisAdd BLAST21
Transmembranei1049 – 1069HelicalSequence analysisAdd BLAST21
Transmembranei1090 – 1110HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • acrosomal membrane Source: UniProtKB-SubCell
  • acrosomal vesicle Source: UniProtKB
  • Golgi apparatus Source: GO_Central
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003219551 – 1183Phospholipid-transporting ATPase FetAAdd BLAST1183

Proteomic databases

PaxDbiA3FIN4.
PRIDEiA3FIN4.

PTM databases

iPTMnetiA3FIN4.
PhosphoSitePlusiA3FIN4.

Expressioni

Tissue specificityi

Highly expressed in testis.1 Publication

Developmental stagei

Not detected in testis until 18 days postpartum. At 22 days postpartum, levels increase and remain constant during adulthood. During spermatogenesis, expressed from pachytene spermatocytes to mature sperm.1 Publication

Gene expression databases

BgeeiENSMUSG00000028457.
CleanExiMM_4930417M19RIK.
ExpressionAtlasiA3FIN4. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000103575.

Structurei

3D structure databases

ProteinModelPortaliA3FIN4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0206. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00860000133706.
HOGENOMiHOG000202528.
HOVERGENiHBG050601.
InParanoidiA3FIN4.
OMAiNETWSLC.
OrthoDBiEOG091G0139.
PhylomeDBiA3FIN4.
TreeFamiTF300654.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR030350. FetA.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PTHR24092:SF52. PTHR24092:SF52. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A3FIN4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKYVKAFVSE ISWDCSWYCS AMQERRNEDR QKEEEERILQ ANNRRFNSLF
60 70 80 90 100
EYPDNSIKTS KYGFFNFLPM NLFEQFQRLA NAYFLILLFL QLVPQISSLA
110 120 130 140 150
WYTTVIPLIV VLSITGVKDA IDDVKRHRSD QQINNRSVSI LVNGRVEEIK
160 170 180 190 200
WRNVQVGDII KLENNHPVTA DMLLLSSSEP YGLTYIETAD LDGETNLKVK
210 220 230 240 250
QAISVTSAME DNLELLSSFN GEVRCDPPNN KLDKFSGTLS YLGNTYLLNH
260 270 280 290 300
ERLLLRGCVI RNTDWCYGLV VYTGQDTKLM QNSGRSTFKR THIDHLMNVL
310 320 330 340 350
VVWIFMFLGG MCFLLSIGHG IWENSRGYYF QAFLPWKHYI TSSATSSALI
360 370 380 390 400
FWSYFIVLNT MVPISLYVSV EIIRLGNSYY INWDRKMFYA PKNMPAQART
410 420 430 440 450
TTLNEELGQV QYVFSDKTGT LTENVMIFNK CSINGKTYGY SYDDNGEYVP
460 470 480 490 500
KSPKDKVDFS YNHLADPKFS FYDKTLVEAV KSEDPLVYLF FLCLSLCHTV
510 520 530 540 550
MSEEKVEGEL VYQAQSPDEG ALVTATRNFG FVFCSRTPET ITVIEMGKIR
560 570 580 590 600
VYRLLAILDF SNERKRMSVI VRTPEDRVML FCKGADTIIY ELLHPSCASL
610 620 630 640 650
SEVTMDHLDD FASEGLRTLM VAYRELDKAY FQTWIKKHGE AWLTLENRER
660 670 680 690 700
KLALVYEEIE RDLMLLGATA IEDKLQRGVP ETIVTLSKAK IKIWVLTGDK
710 720 730 740 750
QETAVNIAYS CRIFKDEMDG VFMVEGTDRE TVLEELRTAR KKMKPESLLE
760 770 780 790 800
SDPINMYLAR KPKMPFKSLD EVANGNYGLV ISGYSLAYAL EGSLEFELLR
810 820 830 840 850
TACMCKGVVC CRMTPLQKAQ VVDLVKRYKK VVTLAIGDGA NDISMIKAAH
860 870 880 890 900
IGVGISNQEG MQATLSSDFS FCQFHFLQRL LLVHGRLSYN RMCKFLSYFF
910 920 930 940 950
YKNFAFTLVH FWYAFFNGFS AQTVYDIWFI TFYNLIYTSL PVLGLSLFEK
960 970 980 990 1000
DVNETWSLCY PELYEPGQHN LYFNKKEFVK CLLHGIYNSF VLFFVPMGTV
1010 1020 1030 1040 1050
FNSERNDGKD ISDFQSFSLL VQTTLIGVMT MQIALRTTSW TMINHTFTWG
1060 1070 1080 1090 1100
SLGLYFCILI LLCSDGLCLR YPSIFNFLGV ARNSLSQPQI WLCLILSTIL
1110 1120 1130 1140 1150
CMIPLIGYNF LRPLLWPINA DKVLNRIHFC LKHPIPTQVQ TKIKHPSLRR
1160 1170 1180
SAYAFSHKQG FGALITSGKT LKSSALAKSK RFL
Length:1,183
Mass (Da):135,899
Last modified:March 20, 2007 - v1
Checksum:iAFB70541AE6A0714
GO
Isoform 2 (identifier: A3FIN4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     304-305: IF → VS
     306-1183: Missing.

Note: No experimental confirmation available.
Show »
Length:305
Mass (Da):35,259
Checksum:i015F7667BA93C2DD
GO
Isoform 3 (identifier: A3FIN4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     454-460: KDKVDFS → VSNYSFL
     461-1183: Missing.

Note: No experimental confirmation available.
Show »
Length:460
Mass (Da):53,163
Checksum:iBFBE91122F61C688
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 33KYVKA…DRQKE → GSNGASSHRTSTLLTGQPSQ HPRLVSEQIPQQ in CAM16528 (PubMed:19468303).CuratedAdd BLAST32

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_031844304 – 305IF → VS in isoform 2. 1 Publication2
Alternative sequenceiVSP_031845306 – 1183Missing in isoform 2. 1 PublicationAdd BLAST878
Alternative sequenceiVSP_031846454 – 460KDKVDFS → VSNYSFL in isoform 3. 1 Publication7
Alternative sequenceiVSP_031847461 – 1183Missing in isoform 3. 1 PublicationAdd BLAST723

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF377342 mRNA. Translation: ABN48718.1.
AK076708 mRNA. Translation: BAC36451.1.
AK078567 mRNA. Translation: BAC37336.1.
AL732504 Genomic DNA. Translation: CAM16526.1.
AL732504 Genomic DNA. Translation: CAM16528.2.
CCDSiCCDS18091.2. [A3FIN4-1]
RefSeqiNP_796169.2. NM_177195.3. [A3FIN4-1]
UniGeneiMm.74465.

Genome annotation databases

EnsembliENSMUST00000102953; ENSMUSP00000100018; ENSMUSG00000028457. [A3FIN4-2]
ENSMUST00000107942; ENSMUSP00000103575; ENSMUSG00000028457. [A3FIN4-1]
GeneIDi320571.
KEGGimmu:320571.
UCSCiuc008spj.1. mouse. [A3FIN4-2]
uc008spk.1. mouse. [A3FIN4-3]
uc008spl.1. mouse. [A3FIN4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF377342 mRNA. Translation: ABN48718.1.
AK076708 mRNA. Translation: BAC36451.1.
AK078567 mRNA. Translation: BAC37336.1.
AL732504 Genomic DNA. Translation: CAM16526.1.
AL732504 Genomic DNA. Translation: CAM16528.2.
CCDSiCCDS18091.2. [A3FIN4-1]
RefSeqiNP_796169.2. NM_177195.3. [A3FIN4-1]
UniGeneiMm.74465.

3D structure databases

ProteinModelPortaliA3FIN4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000103575.

PTM databases

iPTMnetiA3FIN4.
PhosphoSitePlusiA3FIN4.

Proteomic databases

PaxDbiA3FIN4.
PRIDEiA3FIN4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102953; ENSMUSP00000100018; ENSMUSG00000028457. [A3FIN4-2]
ENSMUST00000107942; ENSMUSP00000103575; ENSMUSG00000028457. [A3FIN4-1]
GeneIDi320571.
KEGGimmu:320571.
UCSCiuc008spj.1. mouse. [A3FIN4-2]
uc008spk.1. mouse. [A3FIN4-3]
uc008spl.1. mouse. [A3FIN4-1]

Organism-specific databases

CTDi320571.
MGIiMGI:2444287. Atp8b5.

Phylogenomic databases

eggNOGiKOG0206. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00860000133706.
HOGENOMiHOG000202528.
HOVERGENiHBG050601.
InParanoidiA3FIN4.
OMAiNETWSLC.
OrthoDBiEOG091G0139.
PhylomeDBiA3FIN4.
TreeFamiTF300654.

Miscellaneous databases

ChiTaRSiAfp. mouse.
PROiA3FIN4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028457.
CleanExiMM_4930417M19RIK.
ExpressionAtlasiA3FIN4. baseline and differential.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR030350. FetA.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PTHR24092:SF52. PTHR24092:SF52. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT8B5_MOUSE
AccessioniPrimary (citable) accession number: A3FIN4
Secondary accession number(s): A2AIL8, Q8BVE9, Q8BVS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 20, 2007
Last modified: November 30, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.