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A3EZ55 (HOG1A_WALI9) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase HOG1A

Short name=MAP kinase HOG1A
EC=2.7.11.24
Alternative name(s):
WiHog1A
Gene names
Name:HOG1A
ORF Names:J056_003708
OrganismWallemia ichthyophaga (strain EXF-994 / CBS 113033) [Complete proteome]
Taxonomic identifier1299270 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaBasidiomycota incertae sedisWallemiomycetesWallemialesWallemiales incertae sedisWallemia

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by tyrosine and threonine phosphorylation By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Induction

By hyperosmotic and hypo-osmotic stress. Less induced than isoform HOG1Bunder the same conditions. Ref.1

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Phosphorylated. Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme By similarity. Rapidly dephosphorylated upon either hypo- or hyperosmotic shock. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence ABN54705.1 differs from that shown. Reason: Contains vector sequence in the N- and C-terminal part.

The sequence EOR02032.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   LigandATP-binding
Nucleotide-binding
   Molecular functionActivator
Kinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processregulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 365365Mitogen-activated protein kinase HOG1A
PRO_0000289706

Regions

Domain24 – 303280Protein kinase
Nucleotide binding30 – 389ATP By similarity
Motif175 – 1773TXY

Sites

Active site1451Proton acceptor By similarity
Binding site531ATP By similarity

Amino acid modifications

Modified residue1751Phosphothreonine By similarity
Modified residue1771Phosphotyrosine By similarity

Experimental info

Sequence conflict1841R → K in ABN54705. Ref.3

Sequences

Sequence LengthMass (Da)Tools
A3EZ55 [UniParc].

Last modified May 14, 2014. Version 2.
Checksum: 387C630BB77C6734

FASTA36541,633
        10         20         30         40         50         60 
MSQEDSSFVK LSVFGNIFQV TTRYTDLQPV GMGAFGLLCS STDQKSGGPV AIKKVMKPFS 

        70         80         90        100        110        120 
APVLAKRTYR ELKLLKHLRH ENIISLLDVF ISPGEDIYFI TELLGTDLHR LLSSRPLERQ 

       130        140        150        160        170        180 
FVQYFLYQML RALKFVHPAG VVHRDLKPSN ILINENCDLK ICDFGLARLQ DPQMTGYVST 

       190        200        210        220        230        240 
RYYRAPEIML TWQEYDSAVD IWSVGCIFAE MIDGRPIFPG KDHVHQLTVI TELLGSPPED 

       250        260        270        280        290        300 
VINTITSENT RRFVDALPKR HKISFADRFP NANAEEIDLL EKMLDFNPKK RITAADALAH 

       310        320        330        340        350        360 
PYLAPYHDPE DEPTANERFD WSFNDADLPT DQWKVMMYSE ILDFHNVDVK SEKDMTPSTT 


TAGAH 

« Hide

References

« Hide 'large scale' references
[1]"The HOG signal transduction pathway in the halophilic fungus Wallemia ichthyophaga: identification and characterisation of MAP kinases WiHog1A and WiHog1B."
Konte T., Plemenitas A.
Extremophiles 17:623-636(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION, INDUCTION.
Strain: EXF-994 / CBS 113033.
[2]"Genome and transcriptome sequencing of the halophilic fungus Wallemia ichthyophaga: haloadaptations present and absent."
Zajc J., Liu Y., Dai W., Yang Z., Hu J., Gostincar C., Gunde-Cimerman N.
BMC Genomics 14:617-617(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: EXF-994 / CBS 113033.
[3]"The MAP kinase HwHog1 from the halophilic black yeast Hortaea werneckii: coping with stresses in solar salterns."
Lenassi M., Vaupotic T., Gunde-Cimerman N., Plemenitas A.
Saline Syst. 3:3-3(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 76-338.
Strain: EXF-994 / CBS 113033.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
JX573532 Genomic DNA. Translation: AGG39582.1.
KE007228 Genomic DNA. Translation: EOR02032.1. Sequence problems.
EF158006 Genomic DNA. Translation: ABN54705.1. Sequence problems.

3D structure databases

ProteinModelPortalA3EZ55.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01773. P38MAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHOG1A_WALI9
AccessionPrimary (citable) accession number: A3EZ55
Secondary accession number(s): M1S3C2, R9AIJ2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 14, 2014
Last modified: July 9, 2014
This is version 33 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families