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A3EZ55

- HOG1A_WALI9

UniProt

A3EZ55 - HOG1A_WALI9

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Protein

Mitogen-activated protein kinase HOG1A

Gene

HOG1A

Organism
Wallemia ichthyophaga (strain EXF-994 / CBS 113033)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531ATPPROSITE-ProRule annotation
Active sitei145 – 1451Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 389ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase HOG1A (EC:2.7.11.24)
Short name:
MAP kinase HOG1A
Alternative name(s):
WiHog1A
Gene namesi
Name:HOG1A
ORF Names:J056_003708
OrganismiWallemia ichthyophaga (strain EXF-994 / CBS 113033)
Taxonomic identifieri1299270 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaBasidiomycota incertae sedisWallemiomycetesWallemialesWallemiales incertae sedisWallemia
ProteomesiUP000014064: Unassembled WGS sequence

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Mitogen-activated protein kinase HOG1APRO_0000289706Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei175 – 1751PhosphothreonineBy similarity
Modified residuei177 – 1771PhosphotyrosineBy similarity

Post-translational modificationi

Phosphorylated. Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme (By similarity). Rapidly dephosphorylated upon either hypo- or hyperosmotic shock.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

By hyperosmotic and hypo-osmotic stress. Less induced than isoform HOG1B under the same conditions.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 303280Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi175 – 1773TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3EZ55-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQEDSSFVK LSVFGNIFQV TTRYTDLQPV GMGAFGLLCS STDQKSGGPV
60 70 80 90 100
AIKKVMKPFS APVLAKRTYR ELKLLKHLRH ENIISLLDVF ISPGEDIYFI
110 120 130 140 150
TELLGTDLHR LLSSRPLERQ FVQYFLYQML RALKFVHPAG VVHRDLKPSN
160 170 180 190 200
ILINENCDLK ICDFGLARLQ DPQMTGYVST RYYRAPEIML TWQEYDSAVD
210 220 230 240 250
IWSVGCIFAE MIDGRPIFPG KDHVHQLTVI TELLGSPPED VINTITSENT
260 270 280 290 300
RRFVDALPKR HKISFADRFP NANAEEIDLL EKMLDFNPKK RITAADALAH
310 320 330 340 350
PYLAPYHDPE DEPTANERFD WSFNDADLPT DQWKVMMYSE ILDFHNVDVK
360
SEKDMTPSTT TAGAH
Length:365
Mass (Da):41,633
Last modified:May 14, 2014 - v2
Checksum:i387C630BB77C6734
GO

Sequence cautioni

The sequence ABN54705.1 differs from that shown. Reason: Contains vector sequence in the N- and C-terminal part.
The sequence EOR02032.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti184 – 1841R → K in ABN54705. (PubMed:17349032)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JX573532 Genomic DNA. Translation: AGG39582.1.
KE007228 Genomic DNA. Translation: EOR02032.1. Sequence problems.
EF158006 Genomic DNA. Translation: ABN54705.1. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JX573532 Genomic DNA. Translation: AGG39582.1 .
KE007228 Genomic DNA. Translation: EOR02032.1 . Sequence problems.
EF158006 Genomic DNA. Translation: ABN54705.1 . Sequence problems.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01773. P38MAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The HOG signal transduction pathway in the halophilic fungus Wallemia ichthyophaga: identification and characterisation of MAP kinases WiHog1A and WiHog1B."
    Konte T., Plemenitas A.
    Extremophiles 17:623-636(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION, INDUCTION.
    Strain: EXF-994 / CBS 113033.
  2. "Genome and transcriptome sequencing of the halophilic fungus Wallemia ichthyophaga: haloadaptations present and absent."
    Zajc J., Liu Y., Dai W., Yang Z., Hu J., Gostincar C., Gunde-Cimerman N.
    BMC Genomics 14:617-617(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: EXF-994 / CBS 113033.
  3. "The MAP kinase HwHog1 from the halophilic black yeast Hortaea werneckii: coping with stresses in solar salterns."
    Lenassi M., Vaupotic T., Gunde-Cimerman N., Plemenitas A.
    Saline Syst. 3:3-3(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 76-338.
    Strain: EXF-994 / CBS 113033.

Entry informationi

Entry nameiHOG1A_WALI9
AccessioniPrimary (citable) accession number: A3EZ55
Secondary accession number(s): M1S3C2, R9AIJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 14, 2014
Last modified: October 29, 2014
This is version 35 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3