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A3EZ55

- HOG1A_WALI9

UniProt

A3EZ55 - HOG1A_WALI9

Protein

Mitogen-activated protein kinase HOG1A

Gene

HOG1A

Organism
Wallemia ichthyophaga (strain EXF-994 / CBS 113033)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 2 (14 May 2014)
      Previous versions | rss
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    Functioni

    Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by tyrosine and threonine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531ATPPROSITE-ProRule annotation
    Active sitei145 – 1451Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi30 – 389ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase HOG1A (EC:2.7.11.24)
    Short name:
    MAP kinase HOG1A
    Alternative name(s):
    WiHog1A
    Gene namesi
    Name:HOG1A
    ORF Names:J056_003708
    OrganismiWallemia ichthyophaga (strain EXF-994 / CBS 113033)
    Taxonomic identifieri1299270 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaBasidiomycota incertae sedisWallemiomycetesWallemialesWallemiales incertae sedisWallemia
    ProteomesiUP000014064: Unassembled WGS sequence

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 365365Mitogen-activated protein kinase HOG1APRO_0000289706Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei175 – 1751PhosphothreonineBy similarity
    Modified residuei177 – 1771PhosphotyrosineBy similarity

    Post-translational modificationi

    Phosphorylated. Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme By similarity. Rapidly dephosphorylated upon either hypo- or hyperosmotic shock.By similarity1 Publication

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Inductioni

    By hyperosmotic and hypo-osmotic stress. Less induced than isoform HOG1B under the same conditions.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliA3EZ55.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 303280Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi175 – 1773TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01773. P38MAPKINASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A3EZ55-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQEDSSFVK LSVFGNIFQV TTRYTDLQPV GMGAFGLLCS STDQKSGGPV    50
    AIKKVMKPFS APVLAKRTYR ELKLLKHLRH ENIISLLDVF ISPGEDIYFI 100
    TELLGTDLHR LLSSRPLERQ FVQYFLYQML RALKFVHPAG VVHRDLKPSN 150
    ILINENCDLK ICDFGLARLQ DPQMTGYVST RYYRAPEIML TWQEYDSAVD 200
    IWSVGCIFAE MIDGRPIFPG KDHVHQLTVI TELLGSPPED VINTITSENT 250
    RRFVDALPKR HKISFADRFP NANAEEIDLL EKMLDFNPKK RITAADALAH 300
    PYLAPYHDPE DEPTANERFD WSFNDADLPT DQWKVMMYSE ILDFHNVDVK 350
    SEKDMTPSTT TAGAH 365
    Length:365
    Mass (Da):41,633
    Last modified:May 14, 2014 - v2
    Checksum:i387C630BB77C6734
    GO

    Sequence cautioni

    The sequence ABN54705.1 differs from that shown. Reason: Contains vector sequence in the N- and C-terminal part.
    The sequence EOR02032.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti184 – 1841R → K in ABN54705. (PubMed:17349032)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JX573532 Genomic DNA. Translation: AGG39582.1.
    KE007228 Genomic DNA. Translation: EOR02032.1. Sequence problems.
    EF158006 Genomic DNA. Translation: ABN54705.1. Sequence problems.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JX573532 Genomic DNA. Translation: AGG39582.1 .
    KE007228 Genomic DNA. Translation: EOR02032.1 . Sequence problems.
    EF158006 Genomic DNA. Translation: ABN54705.1 . Sequence problems.

    3D structure databases

    ProteinModelPortali A3EZ55.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01773. P38MAPKINASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The HOG signal transduction pathway in the halophilic fungus Wallemia ichthyophaga: identification and characterisation of MAP kinases WiHog1A and WiHog1B."
      Konte T., Plemenitas A.
      Extremophiles 17:623-636(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION, INDUCTION.
      Strain: EXF-994 / CBS 113033.
    2. "Genome and transcriptome sequencing of the halophilic fungus Wallemia ichthyophaga: haloadaptations present and absent."
      Zajc J., Liu Y., Dai W., Yang Z., Hu J., Gostincar C., Gunde-Cimerman N.
      BMC Genomics 14:617-617(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: EXF-994 / CBS 113033.
    3. "The MAP kinase HwHog1 from the halophilic black yeast Hortaea werneckii: coping with stresses in solar salterns."
      Lenassi M., Vaupotic T., Gunde-Cimerman N., Plemenitas A.
      Saline Syst. 3:3-3(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 76-338.
      Strain: EXF-994 / CBS 113033.

    Entry informationi

    Entry nameiHOG1A_WALI9
    AccessioniPrimary (citable) accession number: A3EZ55
    Secondary accession number(s): M1S3C2, R9AIJ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: May 14, 2014
    Last modified: October 1, 2014
    This is version 34 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3