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Protein

Mitogen-activated protein kinase HOG1A

Gene

HOG1A

Organism
Wallemia ichthyophaga (strain EXF-994 / CBS 113033)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53ATPPROSITE-ProRule annotation1
Active sitei145Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 38ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase HOG1A (EC:2.7.11.24)
Short name:
MAP kinase HOG1A
Alternative name(s):
WiHog1A
Gene namesi
Name:HOG1A
ORF Names:J056_003708
OrganismiWallemia ichthyophaga (strain EXF-994 / CBS 113033)
Taxonomic identifieri1299270 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaWallemiomycetesWallemialesWallemiales incertae sedisWallemia
Proteomesi
  • UP000014064 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002897061 – 365Mitogen-activated protein kinase HOG1AAdd BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei175PhosphothreonineBy similarity1
Modified residuei177PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated. Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme (By similarity). Rapidly dephosphorylated upon either hypo- or hyperosmotic shock.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

By hyperosmotic and hypo-osmotic stress. Less induced than isoform HOG1B under the same conditions.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 303Protein kinasePROSITE-ProRule annotationAdd BLAST280

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi175 – 177TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK04441.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3EZ55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQEDSSFVK LSVFGNIFQV TTRYTDLQPV GMGAFGLLCS STDQKSGGPV
60 70 80 90 100
AIKKVMKPFS APVLAKRTYR ELKLLKHLRH ENIISLLDVF ISPGEDIYFI
110 120 130 140 150
TELLGTDLHR LLSSRPLERQ FVQYFLYQML RALKFVHPAG VVHRDLKPSN
160 170 180 190 200
ILINENCDLK ICDFGLARLQ DPQMTGYVST RYYRAPEIML TWQEYDSAVD
210 220 230 240 250
IWSVGCIFAE MIDGRPIFPG KDHVHQLTVI TELLGSPPED VINTITSENT
260 270 280 290 300
RRFVDALPKR HKISFADRFP NANAEEIDLL EKMLDFNPKK RITAADALAH
310 320 330 340 350
PYLAPYHDPE DEPTANERFD WSFNDADLPT DQWKVMMYSE ILDFHNVDVK
360
SEKDMTPSTT TAGAH
Length:365
Mass (Da):41,633
Last modified:May 14, 2014 - v2
Checksum:i387C630BB77C6734
GO

Sequence cautioni

The sequence ABN54705 differs from that shown. Contains vector sequence in the N- and C-terminal part.Curated
The sequence EOR02032 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184R → K in ABN54705 (PubMed:17349032).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JX573532 Genomic DNA. Translation: AGG39582.1.
KE007228 Genomic DNA. Translation: EOR02032.1. Sequence problems.
EF158006 Genomic DNA. Translation: ABN54705.1. Sequence problems.
RefSeqiXP_009267199.1. XM_009268924.1.

Genome annotation databases

EnsemblFungiiEOR02032; EOR02032; J056_003708.
GeneIDi20376660.
KEGGiwic:J056_003708.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JX573532 Genomic DNA. Translation: AGG39582.1.
KE007228 Genomic DNA. Translation: EOR02032.1. Sequence problems.
EF158006 Genomic DNA. Translation: ABN54705.1. Sequence problems.
RefSeqiXP_009267199.1. XM_009268924.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEOR02032; EOR02032; J056_003708.
GeneIDi20376660.
KEGGiwic:J056_003708.

Phylogenomic databases

KOiK04441.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHOG1A_WALI9
AccessioniPrimary (citable) accession number: A3EZ55
Secondary accession number(s): M1S3C2, R9AIJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 14, 2014
Last modified: May 11, 2016
This is version 41 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.