A3EXH0 (NCAP_BCHK9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoprotein

Alternative name(s):
Nucleocapsid protein
Short name=NC
Short name=Protein N

Gene names

Name:	N
ORF Names:	6

Organism

Bat coronavirus HKU9 (BtCoV) (BtCoV/HKU9) [Complete proteome]

Taxonomic identifier

694006 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Nidovirales › Coronaviridae › Coronavirinae › Betacoronavirus

Virus host

Rousettus leschenaultii (Leschenault's rousette) [TaxID: 9408]

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Interaction with the M protein leads to the formation of virus particles. Binds to cellular membranes and phospholipids. Elicits cell-mediated immunity. May play roles in viral transcription and translation, and/or replication By similarity.
Subunit structure	Monomer and oligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with the M protein By similarity.
Subcellular location	Virion By similarity. Host cytoplasm › host perinuclear region By similarity. Host endoplasmic reticulum-Golgi intermediate compartment By similarity. Host Golgi apparatus By similarity. Note: Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding By similarity.
Sequence similarities	Belongs to the coronavirus group 2b/2c/2d nucleocapsid protein family.

Ontologies

Keywords
Cellular component	Host Golgi apparatus Host cytoplasm Virion
Ligand	RNA-binding Viral nucleoprotein
Molecular function	Ribonucleoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	host cell Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell host cell endoplasmic reticulum-Golgi intermediate compartment Inferred from electronic annotation. Source: UniProtKB-SubCell host cell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW viral nucleocapsid Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Nucleoprotein		PRO_0000291327
Regions
	Region	58 – 193	136	RNA-binding By similarity
	Region	270 – 378	109	Dimerization By similarity
	Compositional bias	189 – 217	29	Ser-rich
	Compositional bias	314 – 317	4	Poly-Ala
	Compositional bias	381 – 387	7	Poly-Lys

Sequences

	Sequence		Length	Mass (Da)
	A3EXH0 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 188D262F3D9FAA0E	FASTA	468	51,547
	10 20 30 40 50 60 MSGRNRSRSG TPSPKVTFKQ ESDGSDSESE RRNGNRNGAR PKNNNSRGSA PKPEKPKAAP 70 80 90 100 110 120 PQNVSWFAPL VQTGKAELRF PRGEGVPVSQ GVDSTYEHGY WLRTQRSFQK GGKQVLANPR 130 140 150 160 170 180 WYFYYTGTGR FGDLRFGTKN PDIVWVGQEG ANINRLGDMG TRNPSNDGAI PVQLAGGIPK 190 200 210 220 230 240 GFYAEGRGSR GNSRSSSRNS SRASSRGNSR ASSRGASPGR PAANPSTEPW MAYLVQKLER 250 260 270 280 290 300 LESQVSGTKP ATKNPVQVTK NEAAANAKKL RHKRTAHKGS GVTVNYGRRG PGDLEGNFGD 310 320 330 340 350 360 REMIKLGTDD PRFAAAAQMA PNVSSFLFMS HLSTRDEDDA LWLHYKGAIK LPKDDPNYEQ 370 380 390 400 410 420 WTKILAENLN AYKDFPPTEP KKDKKKKEET AQDTVIFEDA STGTDQTVVK VWVKDQDAQT 430 440 450 460 DDEWLGGDET VYEDEDDRPK TQRRHKKRGS TASRVTIADP TNAGAERS

References

[1]

"Comparative analysis of twelve genomes of three novel group 2c and group 2d coronaviruses reveals unique group and subgroup features." Woo P.C.Y., Wang M., Lau S.K.P., Xu H.F., Poon R.W.S., Guo R., Wong B.H.L., Gao K., Tsoi H.-W., Huang Y., Li K.S.M., Lam C.S.F., Chan K.-H., Zheng B.-J., Yuen K.-Y. J. Virol. 81:1574-1585(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate HKU9-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF065513 Genomic RNA. Translation: ABN10915.1.
RefSeq	YP_001039975.1. NC_009021.1.
3D structure databases
ProteinModelPortal	A3EXH0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4836017.
Family and domain databases
InterPro	IPR001218. Corona_nucleocap. [Graphical view]
Pfam	PF00937. Corona_nucleoca. 1 hit. [Graphical view]
PIRSF	PIRSF003888. Corona_nucleocap. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NCAP_BCHK9

Accession

Primary (citable) accession number: A3EXH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	March 20, 2007
Last modified:	September 21, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program