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A3DSK9 (NSP3_ROTHL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 3

Short name=NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name=NS34
OrganismRotavirus A (strain Human/Philippines/L26/1987 G12-P1B[4]-I2-R2-C2-M1/M2-A2-N1-T2-E2-H1) (RV-A) [Complete proteome]
Taxonomic identifier10953 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.

Subunit structure

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Sequence similarities

Belongs to the rotavirus A NSP3 family.

Sequence caution

The sequence ABA34239.1 differs from that shown. Reason: Erroneous initiation.

The sequence ABV53270.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Non-structural protein 3
PRO_0000369452

Regions

Region4 – 149146RNA-binding By similarity
Region150 – 20657Dimerization By similarity
Region170 – 23465Interaction with ZC3H7B By similarity
Region208 – 313106Interaction with EIF4G1 By similarity
Coiled coil166 – 23772 Potential

Sequences

Sequence LengthMass (Da)Tools
A3DSK9 [UniParc].

Last modified April 14, 2009. Version 2.
Checksum: 2EDB0F1DC55C5130

FASTA31336,411
        10         20         30         40         50         60 
MLKMESTQQM ASSIINSSFE AAVVAATSTL ELMGIQYDYN EVYTRVKSKF DFVMDDSGVK 

        70         80         90        100        110        120 
NNLMGKAATI DQALNGKFSS SIRNRNWMTD SKTVARLDED VNKLRLLLSS KGIDQKMRVL 

       130        140        150        160        170        180 
NACFNVKRVP GKSSSVIKCT RLMKDKIERG EVEVDDTFVE ERMEIDTIDW KSRYDQLERR 

       190        200        210        220        230        240 
FESLKQRVNE KYNNWVIKAR KVNENMNSLQ NVISQQQAHI NELQIYNNKL ERDLQSKIGS 

       250        260        270        280        290        300 
VISSIEWYLR SMELSDDIKS DIEQQLNSID HINPVNAFDD FESILRNLIS DYDRIFIMFK 

       310 
GLLQQSNYTY TYE 

« Hide

References

[1]"Evolutionary history and global spread of the emerging G12 human rotaviruses."
Rahman M., Matthijnssens J., Yang X., Delbeke T., Arijs I., Taniguchi K., Iturriza-Gomara M., Iftekharuddin N., Azim T., Van Ranst M.
J. Virol. 81:2382-2390(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ146697 Genomic RNA. Translation: ABA34239.1. Different initiation.
EF672593 Genomic RNA. Translation: ABV53270.1. Different initiation.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMSSF69903. SSF69903. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNSP3_ROTHL
AccessionPrimary (citable) accession number: A3DSK9
Secondary accession number(s): B3SRU5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: July 9, 2014
This is version 23 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families