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Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus A (strain Human/Philippines/L26/1987 G12-P1B[4]-I2-R2-C2-M1/M2-A2-N1-T2-E2-H1) (RV-A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Magnesium is required for NTPase activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi153 – 1531MagnesiumSequence Analysis
Metal bindingi171 – 1711MagnesiumSequence Analysis
Active sitei225 – 2251For NTPase activityBy similarity

GO - Molecular functioni

  1. hydrolase activity, acting on acid anhydrides Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
  3. nucleotide binding Source: UniProtKB-KW
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2 (EC:3.6.4.-)
Short name:
NSP2
Alternative name(s):
NCVP3
Non-structural RNA-binding protein 35
Short name:
NS35
OrganismiRotavirus A (strain Human/Philippines/L26/1987 G12-P1B[4]-I2-R2-C2-M1/M2-A2-N1-T2-E2-H1) (RV-A)
Taxonomic identifieri10953 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000001459 Componenti: Genome

Subcellular locationi

Host cytoplasm Curated
Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Non-structural protein 2PRO_0000369537Add
BLAST

Interactioni

Subunit structurei

Homooctamer (By similarity). Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliA3DSK8.
SMRiA3DSK8. Positions 1-313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni205 – 24137RNA-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus NSP2 family.Curated

Family and domain databases

Gene3Di3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProiIPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view]
PfamiPF02509. Rota_NS35. 1 hit.
[Graphical view]
SUPFAMiSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.

Sequencei

Sequence statusi: Complete.

A3DSK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELACFCYP HLENDSYKFI PFNSLAIKCM LTAKVDKKDQ DKFYNSIIYG
60 70 80 90 100
IAPPPQFKKR YNTNDNSRGM NYETSMFNKV AILICEALNS IRVTQSDVAN
110 120 130 140 150
VLSRVVSVRH LENLVLRKEN HQDVLFHSKE LLLKAVLIAI GQSKEIETTA
160 170 180 190 200
TAEGGEIVFQ NAAFTMWKLT YLDHKLMPIL DQNFIEYKIT LNEDKPISDI
210 220 230 240 250
CVKELVAELR WQYNRFAVIT HGKGHYRVIK YSSVANHADR VFATYKNNAK
260 270 280 290 300
SGNVTDFNLL DQRIIWQNWY AFTSSMKQGN TLDVCKKLLF QKMKQEKNPF
310
KGLSTDRKMD EVSHVGI
Length:317
Mass (Da):36,556
Last modified:March 19, 2007 - v1
Checksum:i90CA5180EE18D54C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ146696 Genomic RNA. Translation: ABA34232.1.
EF672594 Genomic RNA. Translation: ABV53271.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ146696 Genomic RNA. Translation: ABA34232.1.
EF672594 Genomic RNA. Translation: ABV53271.1.

3D structure databases

ProteinModelPortaliA3DSK8.
SMRiA3DSK8. Positions 1-313.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProiIPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view]
PfamiPF02509. Rota_NS35. 1 hit.
[Graphical view]
SUPFAMiSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
    Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
    J. Virol. 82:11106-11116(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP2_ROTHL
AccessioniPrimary (citable) accession number: A3DSK8
Secondary accession number(s): B3SRU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2009
Last sequence update: March 19, 2007
Last modified: January 6, 2015
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.