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Protein

Polymerase basic protein 2

Gene

PB2

Organism
Influenza A virus (strain A/USA:Memphis/10/1996 H1N1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. In addition, participates in the inhibition of type I interferon induction through interaction with and inhibition of the host mitochondrial antiviral signaling protein MAVS.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei627Mammalian adaptationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Inhibition of host RNA polymerase II by virus, mRNA capping, mRNA processing, Viral immunoevasion, Viral transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase basic protein 2UniRule annotation
Alternative name(s):
RNA-directed RNA polymerase subunit P3UniRule annotation
Gene namesi
Name:PB2UniRule annotation
OrganismiInfluenza A virus (strain A/USA:Memphis/10/1996 H1N1)
Taxonomic identifieri416730 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000007557 Componenti: Genome

Subcellular locationi

  • Virion UniRule annotation
  • Host nucleus UniRule annotation
  • Host mitochondrion UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host mitochondrion, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003730411 – 759Polymerase basic protein 2Add BLAST759

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). Interacts with nucleoprotein NP (via N-terminus). Interacts (via N-terminus) with host MAVS (via N-terminus); this interaction inhibits host innate immune response.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA3DRQ0.
SMRiA3DRQ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi736 – 739Nuclear localization signalUniRule annotation4

Sequence similaritiesi

Belongs to the influenza viruses PB2 family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900002O.

Family and domain databases

HAMAPiMF_04062. INV_PB2. 1 hit.
InterProiView protein in InterPro
IPR037258. PDB2_C.
IPR001591. RNA_pol_PB2_orthomyxovir.
PfamiView protein in Pfam
PF00604. Flu_PB2. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
SUPFAMiSSF160453. SSF160453. 1 hit.

Sequencei

Sequence statusi: Complete.

A3DRQ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERIKELRNL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPSLRMKWM
60 70 80 90 100
MAMKYPITAD KKITEMIPER NEQGQTLWSK VNDAGSDRVM ISPLAVTWWN
110 120 130 140 150
RNGPVASTIH YPKIYKTYFE KVERLKHGTF GPVHFRNQVK IRRRVDINPG
160 170 180 190 200
HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTITKEKK EELQNCKISP
210 220 230 240 250
LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEV
260 270 280 290 300
RNDDVDQSLI IAARNIVRRA AVSADPLASL LEMCHSTQIG GTRMVDILRQ
310 320 330 340 350
NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKR EEEVLTGNLQ
360 370 380 390 400
TLKLNVHEGY EEFTMVGKRA TAILRKATRR LIQLIVSGRD EQSIVEAIVV
410 420 430 440 450
AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKVLFQNWG
460 470 480 490 500
IEPIDNVMGM IGILPDMTPS TEMSMRGVRV SKMGVDEYSN AERVVVSIDR
510 520 530 540 550
FLRVRDQRGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEIN GPESVLINTY
560 570 580 590 600
QWIIRNWETV KIQWSQNPTM LYNKMEFEPF QSLVPKAIRG QYSGFVRTLF
610 620 630 640 650
QQMRDVLGTF DTIQIIKLLP FAAAPPKQSR MQFSSLTVNV RGSGMRILVR
660 670 680 690 700
GNSPVFNYNK TTKRLTVLGK DAGTLTEDPD EGTAGVESAV LRGFLILGKE
710 720 730 740 750
DRRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA

TKRIRMAIN
Length:759
Mass (Da):85,961
Last modified:March 20, 2007 - v1
Checksum:iDCA32F8B47FBA67D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CY019802 Viral cRNA. Translation: ABN50972.1.

Similar proteinsi

Entry informationi

Entry nameiPB2_I96A2
AccessioniPrimary (citable) accession number: A3DRQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 20, 2007
Last modified: November 22, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families