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Reviewed, UniProtKB/Swiss-Prot A3DN24 (RTCA_STAMF)

Last modified September 1, 2009. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4
Gene names
Name: rtcA
Ordered Locus Names: Smar_0935
OrganismStaphylothermus marinus (strain ATCC 43588 / DSM 3639 / F1) [Complete proteome] [HAMAP]
Taxonomic identifier399550 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeStaphylothermus

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.

Catalytic activity

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processRNA processing

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-3'-phosphate cyclase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200
PRO_0000325193

Sites

Active site3191 By similarity

Sequences

Sequence LengthMass (Da)Tools
A3DN24-1 [UniParc].

Last modified March 18, 2008. Version 2.
Checksum: 784A6E393E0C382E

FASTA35738,755
        10         20         30         40         50         60 
MEIIEIDGSM GEGGGQILRY SLGFSAVTLK PVRIYNIRAK RSNPGLRPQH LTAVKALQMI 

        70         80         90        100        110        120 
TDAVVKGAKV GSMEIYFEPK RRKPGNYRFN IGTAGSTTLV IQAILPALLF SKGYSSVEII 

       130        140        150        160        170        180 
GGTDVPWSPP IDYMRYVFIY NLRFFGVDVE IELYRRGHYP RGGGRVVLKV KPLLGKMKPI 

       190        200        210        220        230        240 
NIVERGKLIS IYGISHAVRL PRHVAERQAR SAATVIREKL GVEPNILIES YPPGRDPHLG 

       250        260        270        280        290        300 
PGSGIVLYAD VEAGTRLGGD ALGARGKRAE IVGREAAEKL IDELSSGMAF DSHMGDMLIP 

       310        320        330        340        350 
YMFLAGGESI AGVSKLTNHT LTAIEVSKIF LPNSKVSIVG NKGEKGIIRI HGVGLSP 

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References

[1]"Complete sequence of Staphylothermus marinus F1."
Copeland A., Lucas S., Lapidus A., Barry K., Dalin E., Tice H., Pitluck S., Sun H., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Huber H., Woese C., Anderson I.J., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

CP000575 Genomic DNA. Translation: ABN70034.1. Different initiation.
RefSeqYP_001040942.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGA3DN24.

Genome annotation databases

GeneID4907365.
GenomeReviewsGene locus Smar_0935 in contig CP000575_GR.
KEGGsmr:Smar_0935.

Organism-specific databases

CMRSearch...

Family and domain databases

HAMAPMF_00200.
[Tree]
InterProIPR000228. RNA3'_term_phos_cycl-like.
IPR013796. RNA3'_term_phos_cycl_insert.
IPR017770. RNA3'_term_phos_cycl_sub.
[Graphical view]
Gene3DG3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHERPTHR11096. RNA3'_term_phos_cycl. 1 hit.
PfamPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
TIGRFAMsTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRTCA_STAMF
AccessionPrimary (citable) accession number: A3DN24
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: September 1, 2009
This is version 18 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents