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Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Staphylothermus marinus (strain ATCC 43588 / DSM 3639 / JCM 9404 / F1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.UniRule annotation

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei84NucleophileUniRule annotation1
Binding sitei116L-glutamineUniRule annotation1
Active sitei185Charge relay systemUniRule annotation1
Active sitei187Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00245.

Protein family/group databases

MEROPSiC26.A32.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxTUniRule annotation (EC:4.3.3.6UniRule annotation)
Alternative name(s):
Pdx2UniRule annotation
Pyridoxal 5'-phosphate synthase glutaminase subunitUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:pdxTUniRule annotation
Ordered Locus Names:Smar_0585
OrganismiStaphylothermus marinus (strain ATCC 43588 / DSM 3639 / JCM 9404 / F1)
Taxonomic identifieri399550 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeStaphylothermus
Proteomesi
  • UP000000254 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002930241 – 205Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST205

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.UniRule annotation

Protein-protein interaction databases

STRINGi399550.Smar_0585.

Structurei

3D structure databases

ProteinModelPortaliA3DM32.
SMRiA3DM32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni52 – 54L-glutamine bindingUniRule annotation3
Regioni145 – 146L-glutamine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00034. Archaea.
COG0311. LUCA.
HOGENOMiHOG000039949.
KOiK08681.
OMAiVFIRAPI.
OrthoDBiPOG093Z0AY8.

Family and domain databases

CDDicd01749. GATase1_PB. 1 hit.
Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01615. PdxT. 1 hit.
InterProiView protein in InterPro
IPR029062. Class_I_gatase-like.
IPR002161. PdxT/SNO.
IPR021196. PdxT/SNO_CS.
PfamiView protein in Pfam
PF01174. SNO. 1 hit.
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiView protein in PROSITE
PS01236. PDXT_SNO_1. 1 hit.
PS51130. PDXT_SNO_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A3DM32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGVLGFQG GVYEHVYMLK KSFHELGING KVLIVKKPQQ LHDLDGIIIP
60 70 80 90 100
GGESTTISIL ARKTNVLELL REKIVEGLPV MGVCAGAIML AKEVVDQVVG
110 120 130 140 150
ETKQPLLGVM DIAVTRNYFG RQRESFELDI VLDELDDKPF RAVFIRAPAI
160 170 180 190 200
TKYWGATKPI GIINYNGEKV VVAARENNKL ALSFHPELTS DTRIHRYFIE

KIIKK
Length:205
Mass (Da):22,954
Last modified:March 20, 2007 - v1
Checksum:iFB17B48E61317C52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000575 Genomic DNA. Translation: ABN69692.1.
RefSeqiWP_011838883.1. NC_009033.1.

Genome annotation databases

EnsemblBacteriaiABN69692; ABN69692; Smar_0585.
GeneIDi4908027.
KEGGismr:Smar_0585.

Similar proteinsi

Entry informationi

Entry nameiPDXT_STAMF
AccessioniPrimary (citable) accession number: A3DM32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: March 20, 2007
Last modified: August 30, 2017
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families