A3DLS2 (A3DLS2_STAMF) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase HAMAP MF_00209
EC=3.6.1.1 HAMAP MF_00209

Alternative name(s):
Pyrophosphate phospho-hydrolase HAMAP MF_00209

Gene names

Name:	ppa HAMAP MF_00209
Ordered Locus Names:	Smar_0473

Organism

Staphylothermus marinus (strain ATCC 43588 / DSM 3639 / F1) [Complete proteome] [HAMAP]

Taxonomic identifier

399550 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Staphylothermus

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity. HAMAP MF_00209
Subcellular location	Cytoplasm By similarity HAMAP MF_00209.
Sequence similarities	Belongs to the PPase family. HAMAP MF_00209

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_00209
Ligand	Magnesium HAMAP MF_00209 Metal-binding HAMAP MF_00209
Molecular function	Hydrolase HAMAP MF_00209 EMBL ABN69582.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	65	1	Magnesium 1 By similarity HAMAP MF_00209
	Metal binding	70	1	Magnesium 1 By similarity HAMAP MF_00209
	Metal binding	70	1	Magnesium 2 By similarity HAMAP MF_00209
	Metal binding	102	1	Magnesium 1 By similarity HAMAP MF_00209

Sequences

	Sequence		Length	Mass (Da)
	A3DLS2 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: E78FDBBAC742E8B4	FASTA	176	20,324
	10 20 30 40 50 60 MIYDKIGPGK NAPKEVHVVI EIPMGSSVKY EIDKETGLVF VDRILFTSMH YPFNYGFIPG 70 80 90 100 110 120 TLEEDGDPVD VLVLGYESLY PGVIIKARPI GVLETEDEKG RDAKIVAVPV EKIDPRFENI 130 140 150 160 170 KDINDLPENF RDRIAHFFEH YKELEKGKWV KVIGWKSRED ALNRIRDAIE RYKRSM

References

[1]

"Complete genome sequence of Staphylothermus marinus Stetter and Fiala 1986 type strain F1." Anderson I.J., Sun H., Lapidus A., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Lucas S., Barry K., Land M., Richardson P., Huber H., Kyrpides N.C. Stand. Genomic Sci. 1:183-188(2009) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"The complete genome sequence of Staphylothermus marinus reveals differences in sulfur metabolism among heterotrophic Crenarchaeota." Anderson I.J., Dharmarajan L., Rodriguez J., Hooper S., Porat I., Ulrich L.E., Elkins J.G., Mavromatis K., Sun H., Land M., Lapidus A., Lucas S., Barry K., Huber H., Zhulin I.B., Whitman W.B., Mukhopadhyay B., Woese C., Bristow J., Kyrpides N. BMC Genomics 10:145-145(2009) [PubMed: 19341479] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000575 Genomic DNA. Translation: ABN69582.1.
RefSeq	YP_001040490.1. NC_009033.1.
3D structure databases
ProteinModelPortal	A3DLS2.
SMR	A3DLS2. Positions 5-171.
ModBase	Search...
Protein-protein interaction databases
STRING	A3DLS2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4907369.
GenomeReviews	Gene locus Smar_0473 in contig CP000575_GR.
KEGG	smr:Smar_0473.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG07782.
HOGENOM	HBG529150.
OMA	PNDFNVI.
PhylomeDB	A3DLS2.
ProtClustDB	CLSK965756.
Enzyme and pathway databases
BioCyc	SMAR399550:SMAR_0473-MONOMER.
Family and domain databases
HAMAP	MF_00209. Inorganic_PPase. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A3DLS2_STAMF

Accession

Primary (citable) accession number: A3DLS2

Entry history

Integrated into UniProtKB/TrEMBL:	March 20, 2007
Last sequence update:	March 20, 2007
Last modified:	November 16, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)