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A3DKS1 (SYL_STAMF) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Smar_0118
OrganismStaphylothermus marinus (strain ATCC 43588 / DSM 3639 / F1) [Complete proteome] [HAMAP]
Taxonomic identifier399550 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeStaphylothermus

Protein attributes

Sequence length969 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 969969Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334848

Regions

Motif45 – 5511"HIGH" region HAMAP-Rule MF_00049
Motif649 – 6535"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6521ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A3DKS1 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: F8EFAE27BDF6D1AB

FASTA969113,418
        10         20         30         40         50         60 
MKKRDFLEWL RSVEAKWQSK WMEKKIFEPR IEPDKPKYFI TVPYPYTNAP LHIGHGRTYT 

        70         80         90        100        110        120 
IGDIIARYKR LRGYNVLFPM AFHITGTPII AISERISRGE EEIINRYKSY IAKYVKDPVE 

       130        140        150        160        170        180 
IEKIIESFKD PLNLAVFFAE RVHMDFDALG YSIDWRRRFH TGEPIYNAFV TWQFLKLREK 

       190        200        210        220        230        240 
GLIKRGDHVV TYCLLHKQPE GEDDIQDADV NPVEILEFTA IKFKLLGEEN TYLVAATLRP 

       250        260        270        280        290        300 
ETLFGATNLW VKPDADYVVV EWRGENIIVS KEALVKLQHQ HPLDEFKVVG EMKGRELVGK 

       310        320        330        340        350        360 
KVVSPLGNEL IVLPADFVDP DNATGIVYSE PSDAPYDYVA LMELKKNSEK LAMYGVDPEV 

       370        380        390        400        410        420 
VKKIEPIKII DVPGIKGHHA GIVVEEMGIS SQFDPRLVEA TKIVYREQYY KGVMIVDDPE 

       430        440        450        460        470        480 
FKGLSVSEAK EKIKKKLLRE NKGFVFYELN RKAYCRAGGK IIAAKIIGQW FIDYSVPWWK 

       490        500        510        520        530        540 
EEAKKYVSEK MRIIPVKYKK AMLDAIDWLE RRPCARKRGL GTRLPFDPEW VIESLSDSTI 

       550        560        570        580        590        600 
YMAFYTIAHL IRKHNIKPEQ LKPQVFDYVF LGKGDPEEIS EDTGIPLKAL EEMRQEFNYW 

       610        620        630        640        650        660 
YPVDQRHTGI AHISNHLSFF IYHHIAIFPR KHWPKMITLN EMVIREGTKM SKSKGNVILL 

       670        680        690        700        710        720 
RDIAEKYSAD LFRLYIAGAA NLDTVLDWRE KEVERVIDSL KKFTAIAEKA IRTKCGTYSH 

       730        740        750        760        770        780 
DKYIDKWFLS KFNRLLAEAT NALDNMEIRD YVQKMFYDVM VSIDHYRERT SNEETICMIK 

       790        800        810        820        830        840 
RILSKWLKSL NPVIPHLTEE IWSWMGKEEF LSLEKWPEID YKAINEEVEY LEEAIEALIE 

       850        860        870        880        890        900 
DIKNVLNILS PKPKHAYIVV ASPWKREVIE MIEKGMDRRE IIRTIRDKYG LKGREKEIVY 

       910        920        930        940        950        960 
VIQECSRTPC KRALKIDPIH EYEAYNEARQ YIAKKTGLHI EVYWEEEAKA KNIPKAEKTL 


PLKPSFYLY 

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References

[1]"The complete genome sequence of Staphylothermus marinus reveals differences in sulfur metabolism among heterotrophic Crenarchaeota."
Anderson I.J., Dharmarajan L., Rodriguez J., Hooper S., Porat I., Ulrich L.E., Elkins J.G., Mavromatis K., Sun H., Land M., Lapidus A., Lucas S., Barry K., Huber H., Zhulin I.B., Whitman W.B., Mukhopadhyay B., Woese C., Bristow J., Kyrpides N.
BMC Genomics 10:145-145(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43588 / DSM 3639 / F1.
[2]"Complete genome sequence of Staphylothermus marinus Stetter and Fiala 1986 type strain F1."
Anderson I.J., Sun H., Lapidus A., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Lucas S., Barry K., Land M., Richardson P., Huber H., Kyrpides N.C.
Stand. Genomic Sci. 1:183-188(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43588 / DSM 3639 / F1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000575 Genomic DNA. Translation: ABN69231.1.
RefSeqYP_001040139.1. NC_009033.1.

3D structure databases

ProteinModelPortalA3DKS1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING399550.Smar_0118.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN69231; ABN69231; Smar_0118.
GeneID4906962.
KEGGsmr:Smar_0118.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAIMNDLRW.
ProtClustDBPRK12300.

Enzyme and pathway databases

BioCycSMAR399550:GHK2-118-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_STAMF
AccessionPrimary (citable) accession number: A3DKS1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 20, 2007
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries