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A3DJE3

- PCKG_CLOTH

UniProt

A3DJE3 - PCKG_CLOTH

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (20 Mar 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei74 – 741SubstrateUniRule annotation
    Binding sitei217 – 2171Substrate; via amide nitrogenUniRule annotation
    Metal bindingi224 – 2241ManganeseUniRule annotation
    Binding sitei224 – 2241SubstrateUniRule annotation
    Metal bindingi244 – 2441Manganese; via tele nitrogenUniRule annotation
    Binding sitei266 – 2661SubstrateUniRule annotation
    Active sitei268 – 2681UniRule annotation
    Metal bindingi291 – 2911ManganeseUniRule annotation
    Binding sitei383 – 3831GTPUniRule annotation
    Binding sitei414 – 4141GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi267 – 2726GTPUniRule annotation
    Nucleotide bindingi510 – 5134GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCTHE203119:GIW8-2981-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:Cthe_2874
    OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
    Taxonomic identifieri203119 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
    ProteomesiUP000002145: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 605605Phosphoenolpyruvate carboxykinase [GTP]PRO_1000080985Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi203119.Cthe_2874.

    Structurei

    3D structure databases

    ProteinModelPortaliA3DJE3.
    SMRiA3DJE3. Positions 6-601.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni381 – 3833Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiRIASKMA.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A3DJE3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSTNMTKNK KLLDWVKEMA EMCQPDEIYW CDGSEEENER LIKLMVDSGL    50
    ATPLNPEKRP GCYLFRSDPS DVARVEDRTF IASKTKEDAG PTNNWIDPVE 100
    LKATMKELYK GCMKGRTMYV IPFSMGPIGS PISKIGVELT DSPYVVVNMR 150
    IMTRIGKAVL DQLGEDGDFV PCLHSVGAPL KEGEKDKGWP CAPIEKKYIS 200
    HFPEERTIWS YGSGYGGNAL LGKKCFALRI ASVMARDEGW LAEHMLILRI 250
    TDPEGNKTYV TGAFPSACGK TNLAMLIPTI PGWKVETIGD DIAWMRFGKD 300
    GRLYAINPEA GFFGVAPGTS MDSNPNAMHT IKKNTIFTNV ALTDDGDVWW 350
    EGIGTEPPAH LIDWQGKDWT PDSGTLAAHP NGRFTAPASQ CPVIAPEWED 400
    PEGVPISAIL IGGRRPNTIP LVHESFDWNH GVFMGSIMGS EITAAAISNK 450
    IGQVRRDPFA MLPFIGYNVN DYLQHWLNMG TKTDPSKLPK IFYVNWFRKD 500
    SNGKWLWPGY GENSRVLKWI VERVNGKGKA VKTPIGYMPT VDAIDTTGLD 550
    VSKEDMEELL SVNKEQWLQE VESIKEHYKS YGEKLPKELW AQLEALEQRL 600
    KEYNG 605
    Length:605
    Mass (Da):67,627
    Last modified:March 20, 2007 - v1
    Checksum:iE3FC7A2A3A4125DA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000568 Genomic DNA. Translation: ABN54072.1.
    RefSeqiYP_001039265.1. NC_009012.1.

    Genome annotation databases

    EnsemblBacteriaiABN54072; ABN54072; Cthe_2874.
    GeneIDi4809154.
    KEGGicth:Cthe_2874.
    PATRICi19519846. VBICloThe47081_3075.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000568 Genomic DNA. Translation: ABN54072.1 .
    RefSeqi YP_001039265.1. NC_009012.1.

    3D structure databases

    ProteinModelPortali A3DJE3.
    SMRi A3DJE3. Positions 6-601.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 203119.Cthe_2874.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN54072 ; ABN54072 ; Cthe_2874 .
    GeneIDi 4809154.
    KEGGi cth:Cthe_2874.
    PATRICi 19519846. VBICloThe47081_3075.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi RIASKMA.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci CTHE203119:GIW8-2981-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

    Entry informationi

    Entry nameiPCKG_CLOTH
    AccessioniPrimary (citable) accession number: A3DJE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3