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A3DJE3 (PCKG_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]

Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Gene names
Name:pckG
Ordered Locus Names:Cthe_2874
OrganismClostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]
Taxonomic identifier203119 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length605 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 605605Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
PRO_1000080985

Regions

Nucleotide binding267 – 2726GTP By similarity
Nucleotide binding510 – 5134GTP By similarity
Region381 – 3833Substrate binding By similarity

Sites

Active site2681 By similarity
Metal binding2241Manganese By similarity
Metal binding2441Manganese; via tele nitrogen By similarity
Metal binding2911Manganese By similarity
Binding site741Substrate By similarity
Binding site2171Substrate; via amide nitrogen By similarity
Binding site2241Substrate By similarity
Binding site2661Substrate By similarity
Binding site3831GTP By similarity
Binding site4141GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
A3DJE3 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: E3FC7A2A3A4125DA

FASTA60567,627
        10         20         30         40         50         60 
MTSTNMTKNK KLLDWVKEMA EMCQPDEIYW CDGSEEENER LIKLMVDSGL ATPLNPEKRP 

        70         80         90        100        110        120 
GCYLFRSDPS DVARVEDRTF IASKTKEDAG PTNNWIDPVE LKATMKELYK GCMKGRTMYV 

       130        140        150        160        170        180 
IPFSMGPIGS PISKIGVELT DSPYVVVNMR IMTRIGKAVL DQLGEDGDFV PCLHSVGAPL 

       190        200        210        220        230        240 
KEGEKDKGWP CAPIEKKYIS HFPEERTIWS YGSGYGGNAL LGKKCFALRI ASVMARDEGW 

       250        260        270        280        290        300 
LAEHMLILRI TDPEGNKTYV TGAFPSACGK TNLAMLIPTI PGWKVETIGD DIAWMRFGKD 

       310        320        330        340        350        360 
GRLYAINPEA GFFGVAPGTS MDSNPNAMHT IKKNTIFTNV ALTDDGDVWW EGIGTEPPAH 

       370        380        390        400        410        420 
LIDWQGKDWT PDSGTLAAHP NGRFTAPASQ CPVIAPEWED PEGVPISAIL IGGRRPNTIP 

       430        440        450        460        470        480 
LVHESFDWNH GVFMGSIMGS EITAAAISNK IGQVRRDPFA MLPFIGYNVN DYLQHWLNMG 

       490        500        510        520        530        540 
TKTDPSKLPK IFYVNWFRKD SNGKWLWPGY GENSRVLKWI VERVNGKGKA VKTPIGYMPT 

       550        560        570        580        590        600 
VDAIDTTGLD VSKEDMEELL SVNKEQWLQE VESIKEHYKS YGEKLPKELW AQLEALEQRL 


KEYNG 

« Hide

References

[1]"Complete sequence of Clostridium thermocellum ATCC 27405."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27405 / DSM 1237.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000568 Genomic DNA. Translation: ABN54072.1.
RefSeqYP_001039265.1. NC_009012.1.

3D structure databases

ProteinModelPortalA3DJE3.
SMRA3DJE3. Positions 6-601.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203119.Cthe_2874.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN54072; ABN54072; Cthe_2874.
GeneID4809154.
KEGGcth:Cthe_2874.
PATRIC19519846. VBICloThe47081_3075.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
KOK01596.
OMARIASKMA.
OrthoDBEOG6MPWQS.

Enzyme and pathway databases

BioCycCTHE203119:GIW8-2981-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_CLOTH
AccessionPrimary (citable) accession number: A3DJE3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 20, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways