Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A3DJE3

- PCKG_CLOTH

UniProt

A3DJE3 - PCKG_CLOTH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741SubstrateUniRule annotation
Binding sitei217 – 2171Substrate; via amide nitrogenUniRule annotation
Metal bindingi224 – 2241ManganeseUniRule annotation
Binding sitei224 – 2241SubstrateUniRule annotation
Metal bindingi244 – 2441Manganese; via tele nitrogenUniRule annotation
Binding sitei266 – 2661SubstrateUniRule annotation
Active sitei268 – 2681UniRule annotation
Metal bindingi291 – 2911ManganeseUniRule annotation
Binding sitei383 – 3831GTPUniRule annotation
Binding sitei414 – 4141GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi267 – 2726GTPUniRule annotation
Nucleotide bindingi510 – 5134GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-2981-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Cthe_2874
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
ProteomesiUP000002145: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 605605Phosphoenolpyruvate carboxykinase [GTP]PRO_1000080985Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi203119.Cthe_2874.

Structurei

3D structure databases

ProteinModelPortaliA3DJE3.
SMRiA3DJE3. Positions 6-601.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni381 – 3833Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiRIASKMA.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3DJE3 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSTNMTKNK KLLDWVKEMA EMCQPDEIYW CDGSEEENER LIKLMVDSGL
60 70 80 90 100
ATPLNPEKRP GCYLFRSDPS DVARVEDRTF IASKTKEDAG PTNNWIDPVE
110 120 130 140 150
LKATMKELYK GCMKGRTMYV IPFSMGPIGS PISKIGVELT DSPYVVVNMR
160 170 180 190 200
IMTRIGKAVL DQLGEDGDFV PCLHSVGAPL KEGEKDKGWP CAPIEKKYIS
210 220 230 240 250
HFPEERTIWS YGSGYGGNAL LGKKCFALRI ASVMARDEGW LAEHMLILRI
260 270 280 290 300
TDPEGNKTYV TGAFPSACGK TNLAMLIPTI PGWKVETIGD DIAWMRFGKD
310 320 330 340 350
GRLYAINPEA GFFGVAPGTS MDSNPNAMHT IKKNTIFTNV ALTDDGDVWW
360 370 380 390 400
EGIGTEPPAH LIDWQGKDWT PDSGTLAAHP NGRFTAPASQ CPVIAPEWED
410 420 430 440 450
PEGVPISAIL IGGRRPNTIP LVHESFDWNH GVFMGSIMGS EITAAAISNK
460 470 480 490 500
IGQVRRDPFA MLPFIGYNVN DYLQHWLNMG TKTDPSKLPK IFYVNWFRKD
510 520 530 540 550
SNGKWLWPGY GENSRVLKWI VERVNGKGKA VKTPIGYMPT VDAIDTTGLD
560 570 580 590 600
VSKEDMEELL SVNKEQWLQE VESIKEHYKS YGEKLPKELW AQLEALEQRL

KEYNG
Length:605
Mass (Da):67,627
Last modified:March 20, 2007 - v1
Checksum:iE3FC7A2A3A4125DA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000568 Genomic DNA. Translation: ABN54072.1.
RefSeqiYP_001039265.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN54072; ABN54072; Cthe_2874.
GeneIDi4809154.
KEGGicth:Cthe_2874.
PATRICi19519846. VBICloThe47081_3075.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000568 Genomic DNA. Translation: ABN54072.1 .
RefSeqi YP_001039265.1. NC_009012.1.

3D structure databases

ProteinModelPortali A3DJE3.
SMRi A3DJE3. Positions 6-601.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203119.Cthe_2874.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN54072 ; ABN54072 ; Cthe_2874 .
GeneIDi 4809154.
KEGGi cth:Cthe_2874.
PATRICi 19519846. VBICloThe47081_3075.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi RIASKMA.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci CTHE203119:GIW8-2981-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

Entry informationi

Entry nameiPCKG_CLOTH
AccessioniPrimary (citable) accession number: A3DJE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 20, 2007
Last modified: October 29, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3