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A3DJE3

- PCKG_CLOTH

UniProt

A3DJE3 - PCKG_CLOTH

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Protein
Phosphoenolpyruvate carboxykinase [GTP]
Gene
pckG, Cthe_2874
Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741Substrate By similarity
Binding sitei217 – 2171Substrate; via amide nitrogen By similarity
Metal bindingi224 – 2241Manganese By similarity
Binding sitei224 – 2241Substrate By similarity
Metal bindingi244 – 2441Manganese; via tele nitrogen By similarity
Binding sitei266 – 2661Substrate By similarity
Active sitei268 – 2681 By similarity
Metal bindingi291 – 2911Manganese By similarity
Binding sitei383 – 3831GTP By similarity
Binding sitei414 – 4141GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi267 – 2726GTP By similarity
Nucleotide bindingi510 – 5134GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-2981-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEP carboxykinase
Short name:
PEPCK
Gene namesi
Name:pckG
Ordered Locus Names:Cthe_2874
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
ProteomesiUP000002145: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 605605Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation
PRO_1000080985Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi203119.Cthe_2874.

Structurei

3D structure databases

ProteinModelPortaliA3DJE3.
SMRiA3DJE3. Positions 6-601.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni381 – 3833Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiRIASKMA.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3DJE3-1 [UniParc]FASTAAdd to Basket

« Hide

MTSTNMTKNK KLLDWVKEMA EMCQPDEIYW CDGSEEENER LIKLMVDSGL    50
ATPLNPEKRP GCYLFRSDPS DVARVEDRTF IASKTKEDAG PTNNWIDPVE 100
LKATMKELYK GCMKGRTMYV IPFSMGPIGS PISKIGVELT DSPYVVVNMR 150
IMTRIGKAVL DQLGEDGDFV PCLHSVGAPL KEGEKDKGWP CAPIEKKYIS 200
HFPEERTIWS YGSGYGGNAL LGKKCFALRI ASVMARDEGW LAEHMLILRI 250
TDPEGNKTYV TGAFPSACGK TNLAMLIPTI PGWKVETIGD DIAWMRFGKD 300
GRLYAINPEA GFFGVAPGTS MDSNPNAMHT IKKNTIFTNV ALTDDGDVWW 350
EGIGTEPPAH LIDWQGKDWT PDSGTLAAHP NGRFTAPASQ CPVIAPEWED 400
PEGVPISAIL IGGRRPNTIP LVHESFDWNH GVFMGSIMGS EITAAAISNK 450
IGQVRRDPFA MLPFIGYNVN DYLQHWLNMG TKTDPSKLPK IFYVNWFRKD 500
SNGKWLWPGY GENSRVLKWI VERVNGKGKA VKTPIGYMPT VDAIDTTGLD 550
VSKEDMEELL SVNKEQWLQE VESIKEHYKS YGEKLPKELW AQLEALEQRL 600
KEYNG 605
Length:605
Mass (Da):67,627
Last modified:March 20, 2007 - v1
Checksum:iE3FC7A2A3A4125DA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000568 Genomic DNA. Translation: ABN54072.1.
RefSeqiYP_001039265.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN54072; ABN54072; Cthe_2874.
GeneIDi4809154.
KEGGicth:Cthe_2874.
PATRICi19519846. VBICloThe47081_3075.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000568 Genomic DNA. Translation: ABN54072.1 .
RefSeqi YP_001039265.1. NC_009012.1.

3D structure databases

ProteinModelPortali A3DJE3.
SMRi A3DJE3. Positions 6-601.
ModBasei Search...

Protein-protein interaction databases

STRINGi 203119.Cthe_2874.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN54072 ; ABN54072 ; Cthe_2874 .
GeneIDi 4809154.
KEGGi cth:Cthe_2874.
PATRICi 19519846. VBICloThe47081_3075.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi RIASKMA.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci CTHE203119:GIW8-2981-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27405 / DSM 1237.

Entry informationi

Entry nameiPCKG_CLOTH
AccessioniPrimary (citable) accession number: A3DJE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 20, 2007
Last modified: September 3, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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