A3DJ77 (GUNC_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase C
EC=3.2.1.4

Alternative name(s):
Cellulase C
Endo-1,4-beta-glucanase C
Short name=EgC

Gene names

Name:	celC
Ordered Locus Names:	Cthe_2807

Organism

Clostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]

Taxonomic identifier

203119 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium thermocellum

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans By similarity.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Pathway	Glycan metabolism; cellulose degradation.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Endoglucanase C		PRO_0000284720
Sites
	Active site	140	1	Proton donor By similarity
	Active site	280	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3DJ77 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 72DD9BEA01A0DD05	FASTA	343	40,905
	10 20 30 40 50 60 MVSFKAGINL GGWISQYQVF SKEHFDTFIT EKDIETIAEA GFDHVRLPFD YPIIESDDNV 70 80 90 100 110 120 GEYKEDGLSY IDRCLEWCKK YNLGLVLDMH HAPGYRFQDF KTSTLFEDPN QQKRFVDIWR 130 140 150 160 170 180 FLAKRYINER EHIAFELLNE VVEPDSTRWN KLMLECVKAI REIDSTRWLY IGGNNYNSPD 190 200 210 220 230 240 ELKNLADIDD DYIVYNFHFY NPFFFTHQKA HWSESAMAYN RTVKYPGQYE GIEEFVKNNP 250 260 270 280 290 300 KYSFMMELNN LKLNKELLRK DLKPAIEFRE KKKCKLYCGE FGVIAIADLE SRIKWHEDYI 310 320 330 340 SLLEEYDIGG AVWNYKKMDF EIYNEDRKPV SQELVNILAR RKT

References

[1]

"Complete sequence of Clostridium thermocellum ATCC 27405." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27405 / DSM 1237.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000568 Genomic DNA. Translation: ABN54006.1.
RefSeq	YP_001039199.1. NC_009012.1.
3D structure databases
ProteinModelPortal	A3DJ77.
SMR	A3DJ77. Positions 1-340.
ModBase	Search...
Protein-protein interaction databases
STRING	203119.Cthe_2807.
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABN54006; ABN54006; Cthe_2807.
GeneID	4809644.
KEGG	cth:Cthe_2807.
PATRIC	19519672. VBICloThe47081_2989.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2730.
HOGENOM	HOG000069582.
OMA	ADRNVVY.
Enzyme and pathway databases
BioCyc	CTHE203119:GIW8-2909-MONOMER.
UniPathway	UPA00696.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNC_CLOTH

Accession

Primary (citable) accession number: A3DJ77
Secondary accession number(s): P07985

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	March 20, 2007
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program