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A3DJ38

- A3DJ38_CLOTH

UniProt

A3DJ38 - A3DJ38_CLOTH

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Protein
Submitted name:

Metallophosphoesterase

Gene
Cthe_2768
Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237)
Status
Unreviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161ADPImported
Binding sitei116 – 1161ATPImported
Binding sitei116 – 1161CTP
Binding sitei116 – 1161GTPImported
Binding sitei120 – 1201ADPImported
Binding sitei162 – 1621GTPImported
Metal bindingi187 – 1871ManganeseImported
Metal bindingi189 – 1891Manganese; via tele nitrogenImported
Metal bindingi233 – 2331ManganeseImported
Binding sitei531 – 5311ATPImported
Binding sitei536 – 5361ADPImported
Binding sitei536 – 5361AMPImported
Binding sitei536 – 5361ATPImported
Binding sitei565 – 5651ADPImported
Binding sitei565 – 5651ATPImported
Binding sitei607 – 6071ADPImported
Binding sitei607 – 6071AMPImported
Binding sitei607 – 6071ATPImported
Binding sitei687 – 6871AMPImported
Binding sitei704 – 7041ADPImported
Binding sitei704 – 7041AMPImported
Binding sitei704 – 7041ATPImported
Metal bindingi731 – 7311Magnesium; via carbonyl oxygenImported
Metal bindingi734 – 7341Magnesium; via carbonyl oxygenImported
Metal bindingi737 – 7371Magnesium; via carbonyl oxygenImported
Binding sitei769 – 7691AMPImported
Binding sitei774 – 7741ADPImported
Binding sitei774 – 7741AMPImported
Binding sitei774 – 7741ATPImported
Binding sitei792 – 7921ADPImported
Binding sitei792 – 7921AMPImported
Binding sitei792 – 7921ATPImported
Binding sitei824 – 8241ADPImported

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 237ATPImported
Nucleotide bindingi17 – 237CTP
Nucleotide bindingi17 – 237GTPImported
Nucleotide bindingi18 – 236ADPImported
Nucleotide bindingi120 – 1234ATPImported
Nucleotide bindingi120 – 1234CTP
Nucleotide bindingi120 – 1234GTPImported
Nucleotide bindingi495 – 4962ADPImported
Nucleotide bindingi529 – 5324AMPImported

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. GTP binding Source: UniProtKB-KW
  3. hydrolase activity Source: InterPro
  4. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

ATP-bindingImported, GTP-bindingImported, MagnesiumImported, ManganeseImported, Metal-bindingImported, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-2869-MONOMER.

Names & Taxonomyi

Protein namesi
Submitted name:
MetallophosphoesteraseImported
Gene namesi
Ordered Locus Names:Cthe_2768Imported
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237)Imported
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
ProteomesiUP000002145: Chromosome

Interactioni

Protein-protein interaction databases

DIPiDIP-60067N.
STRINGi203119.Cthe_2768.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TY5X-ray2.40A/B479-870[»]
3TY8X-ray2.60A/B479-870[»]
3TY9X-ray3.12A/B/C/D479-870[»]
4DRFX-ray2.60A/C445-870[»]
4E6NX-ray2.39A/C445-870[»]
4GP6X-ray2.10A/B1-170[»]
4GP7X-ray2.00A/B1-170[»]
4J6OX-ray1.60A/B171-424[»]
4JSTX-ray2.03A/B1-170[»]
4JSYX-ray2.14A/B1-170[»]
4JT2X-ray2.49A/B1-170[»]
4JT4X-ray2.01A/B1-170[»]
4MDEX-ray1.80A/B1-170[»]
4MDFX-ray1.73A/B1-170[»]
ProteinModelPortaliA3DJ38.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000245786.
KOiK01090.
OMAiHGCRSEL.
OrthoDBiEOG6WX4P8.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR027417. P-loop_NTPase.
IPR024028. PNKP_bac.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF56300. SSF56300. 1 hit.
TIGRFAMsiTIGR04075. bacter_Pnkp. 1 hit.

Sequencei

Sequence statusi: Complete.

A3DJ38-1 [UniParc]FASTAAdd to Basket

« Hide

MKLTIPELSL VVLIGSSGSG KSTFAKKHFK PTEVISSDFC RGLVSDDEND    50
QTVTGAAFDV LHYIVSKRLQ LGKLTVVDAT NVQESARKPL IEIAKDYHCF 100
PVAVVFNLPE KVCQERNKNR TDRQVEEYVI RKHTQQLKKS IKGLQREGFR 150
YVYILNSPEE VEEVVFERQP LWNNKKDEHG PFDIIGDIHG CYDELKMLLE 200
KLGYLIEEVE GGVGSGKYRV THPEGRKVLF LGDLVDRGPK ITEVLKLVMG 250
MVKSGIALCV PGNHDVKLLR KLNGRDVQIT HGLDRTLEQL AKEPQEFIEE 300
VKAFIDGLVS HYVLDDGKLV VAHAGMKEEF QGRGSGKVRE FALYGETTGE 350
TDEYGLPVRY DWASDYRGKA LVVYGHTPQA EVLKVNNTIN IDTGCVFGGK 400
LTAYRYPERE IVDVKALKTY YEPAKPFLPK EDMAERFEAR TDNDILDIND 450
VLGKKIITTR LMSSITIHEE NSIAALEVMS RFAADPHWLI YLPPTMSPCE 500
TSKKEGMLEH PIEAFEYFRT RGVGKVVCEQ KHMGSRAVVI VCKDSQVAEK 550
RFGVLDGTAG ICYTRTGRHF FDDMQLEAEL IDRVRKVLDK SGFWGDFNTD 600
WVCLDCELMP WSAKAQKLLE EQYSAVGISG RVVLDEAVKL LKQASLNKTV 650
SFDVSRQTSG KNADINELLQ RFTERSEMMQ KYVEAYRKYC WPVNSIDDLK 700
LAPFHILATE GKVHSDKNHI WHMDTIAKYC TQDDSLIMAT NHILVDVTDA 750
ESVDKGIKWW EDLTASGGEG MVVKPYDFIV KNGRELLQPA VKCRGREYLR 800
IIYGPEYTMD ENIERLRNRA VGKKRSLALR EFSLGMEALE RFVRNEPLYR 850
VHECVFGVLA LESEPVDPRL 870
Length:870
Mass (Da):98,776
Last modified:March 20, 2007 - v1
Checksum:i11CA5878DD2919E9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000568 Genomic DNA. Translation: ABN53967.1.
RefSeqiWP_020457916.1. NC_009012.1.
YP_001039160.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN53967; ABN53967; Cthe_2768.
GeneIDi4810085.
KEGGicth:Cthe_2768.
PATRICi19519588. VBICloThe47081_2947.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000568 Genomic DNA. Translation: ABN53967.1 .
RefSeqi WP_020457916.1. NC_009012.1.
YP_001039160.1. NC_009012.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3TY5 X-ray 2.40 A/B 479-870 [» ]
3TY8 X-ray 2.60 A/B 479-870 [» ]
3TY9 X-ray 3.12 A/B/C/D 479-870 [» ]
4DRF X-ray 2.60 A/C 445-870 [» ]
4E6N X-ray 2.39 A/C 445-870 [» ]
4GP6 X-ray 2.10 A/B 1-170 [» ]
4GP7 X-ray 2.00 A/B 1-170 [» ]
4J6O X-ray 1.60 A/B 171-424 [» ]
4JST X-ray 2.03 A/B 1-170 [» ]
4JSY X-ray 2.14 A/B 1-170 [» ]
4JT2 X-ray 2.49 A/B 1-170 [» ]
4JT4 X-ray 2.01 A/B 1-170 [» ]
4MDE X-ray 1.80 A/B 1-170 [» ]
4MDF X-ray 1.73 A/B 1-170 [» ]
ProteinModelPortali A3DJ38.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60067N.
STRINGi 203119.Cthe_2768.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN53967 ; ABN53967 ; Cthe_2768 .
GeneIDi 4810085.
KEGGi cth:Cthe_2768.
PATRICi 19519588. VBICloThe47081_2947.

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000245786.
KOi K01090.
OMAi HGCRSEL.
OrthoDBi EOG6WX4P8.

Enzyme and pathway databases

BioCyci CTHE203119:GIW8-2869-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR027417. P-loop_NTPase.
IPR024028. PNKP_bac.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF56300. SSF56300. 1 hit.
TIGRFAMsi TIGR04075. bacter_Pnkp. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27405 / DSM 1237.
  2. "The adenylyltransferase domain of bacterial Pnkp defines a unique RNA ligase family."
    Smith P., Wang L.K., Nair P.A., Shuman S.
    Proc. Natl. Acad. Sci. U.S.A. 109:2296-2301(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 479-870 IN COMPLEX WITH ADP; AMP; ATP AND MAGNESIUM.
  3. "Molecular basis of bacterial protein Hen1 activating the ligase activity of bacterial protein Pnkp for RNA repair."
    Wang P., Chan C.M., Christensen D., Zhang C., Selvadurai K., Huang R.H.
    Proc. Natl. Acad. Sci. U.S.A. 109:13248-13253(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.39 ANGSTROMS) OF 445-870 IN COMPLEX WITH AMP.
  4. "Structure and mechanism of the polynucleotide kinase component of the bacterial Pnkp-Hen1 RNA repair system."
    Wang L.K., Das U., Smith P., Shuman S.
    RNA 18:2277-2286(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 1-170 IN COMPLEX WITH ADP AND ATP.
  5. "Structural and biochemical analysis of the phosphate donor specificity of the polynucleotide kinase component of the bacterial pnkp.hen1 RNA repair system."
    Das U., Wang L.K., Smith P., Shuman S.
    Biochemistry 52:4734-4743(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) OF 1-170 IN COMPLEX WITH CTP AND GTP.
  6. "Structure and mechanism of the 2',3' phosphatase component of the bacterial Pnkp-Hen1 RNA repair system."
    Wang L.K., Smith P., Shuman S.
    Nucleic Acids Res. 41:5864-5873(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 171-424 IN COMPLEX WITH MANGANESE.
  7. "Structures of bacterial polynucleotide kinase in a Michaelis complex with GTP.Mg2+ and 5'-OH oligonucleotide and a product complex with GDP.Mg2+ and 5'-PO4 oligonucleotide reveal a mechanism of general acid-base catalysis and the determinants of phosphoacceptor recognition."
    Das U., Wang L.K., Smith P., Jacewicz A., Shuman S.
    Nucleic Acids Res. 42:1152-1161(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.73 ANGSTROMS) OF 1-170 IN COMPLEX WITH GTP.

Entry informationi

Entry nameiA3DJ38_CLOTH
AccessioniPrimary (citable) accession number: A3DJ38
Entry historyi
Integrated into UniProtKB/TrEMBL: March 20, 2007
Last sequence update: March 20, 2007
Last modified: September 3, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, Complete proteome

External Data

Dasty 3

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