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A3DJ38

- A3DJ38_CLOTH

UniProt

A3DJ38 - A3DJ38_CLOTH

Protein
Submitted name:

Metallophosphoesterase

Gene

Cthe_2768

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (20 Mar 2007)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei116 – 1161ADPImported
    Binding sitei116 – 1161ATPImported
    Binding sitei116 – 1161CTP
    Binding sitei116 – 1161GTPImported
    Binding sitei120 – 1201ADPImported
    Binding sitei162 – 1621GTPImported
    Metal bindingi187 – 1871ManganeseImported
    Metal bindingi189 – 1891Manganese; via tele nitrogenImported
    Metal bindingi233 – 2331ManganeseImported
    Binding sitei531 – 5311ATPImported
    Binding sitei536 – 5361ADPImported
    Binding sitei536 – 5361AMPImported
    Binding sitei536 – 5361ATPImported
    Binding sitei565 – 5651ADPImported
    Binding sitei565 – 5651ATPImported
    Binding sitei607 – 6071ADPImported
    Binding sitei607 – 6071AMPImported
    Binding sitei607 – 6071ATPImported
    Binding sitei687 – 6871AMPImported
    Binding sitei704 – 7041ADPImported
    Binding sitei704 – 7041AMPImported
    Binding sitei704 – 7041ATPImported
    Metal bindingi731 – 7311Magnesium; via carbonyl oxygenImported
    Metal bindingi734 – 7341Magnesium; via carbonyl oxygenImported
    Metal bindingi737 – 7371Magnesium; via carbonyl oxygenImported
    Binding sitei769 – 7691AMPImported
    Binding sitei774 – 7741ADPImported
    Binding sitei774 – 7741AMPImported
    Binding sitei774 – 7741ATPImported
    Binding sitei792 – 7921ADPImported
    Binding sitei792 – 7921AMPImported
    Binding sitei792 – 7921ATPImported
    Binding sitei824 – 8241ADPImported

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi17 – 237ATPImported
    Nucleotide bindingi17 – 237CTP
    Nucleotide bindingi17 – 237GTPImported
    Nucleotide bindingi18 – 236ADPImported
    Nucleotide bindingi120 – 1234ATPImported
    Nucleotide bindingi120 – 1234CTP
    Nucleotide bindingi120 – 1234GTPImported
    Nucleotide bindingi495 – 4962ADPImported
    Nucleotide bindingi529 – 5324AMPImported

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. GTP binding Source: UniProtKB-KW
    3. hydrolase activity Source: InterPro
    4. metal ion binding Source: UniProtKB-KW

    Keywords - Ligandi

    ATP-bindingImported, GTP-bindingImported, MagnesiumImported, ManganeseImported, Metal-bindingImported, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCTHE203119:GIW8-2869-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    MetallophosphoesteraseImported
    Gene namesi
    Ordered Locus Names:Cthe_2768Imported
    OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)Imported
    Taxonomic identifieri203119 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
    ProteomesiUP000002145: Chromosome

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-60067N.
    STRINGi203119.Cthe_2768.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3TY5X-ray2.40A/B479-870[»]
    3TY8X-ray2.60A/B479-870[»]
    3TY9X-ray3.12A/B/C/D479-870[»]
    4DRFX-ray2.60A/C445-870[»]
    4E6NX-ray2.39A/C445-870[»]
    4GP6X-ray2.10A/B1-170[»]
    4GP7X-ray2.00A/B1-170[»]
    4J6OX-ray1.60A/B171-424[»]
    4JSTX-ray2.03A/B1-170[»]
    4JSYX-ray2.14A/B1-170[»]
    4JT2X-ray2.49A/B1-170[»]
    4JT4X-ray2.01A/B1-170[»]
    4MDEX-ray1.80A/B1-170[»]
    4MDFX-ray1.73A/B1-170[»]
    ProteinModelPortaliA3DJ38.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000245786.
    KOiK01090.
    OMAiHGCRSEL.
    OrthoDBiEOG6WX4P8.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR027417. P-loop_NTPase.
    IPR024028. PNKP_bac.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF56300. SSF56300. 1 hit.
    TIGRFAMsiTIGR04075. bacter_Pnkp. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A3DJ38-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLTIPELSL VVLIGSSGSG KSTFAKKHFK PTEVISSDFC RGLVSDDEND    50
    QTVTGAAFDV LHYIVSKRLQ LGKLTVVDAT NVQESARKPL IEIAKDYHCF 100
    PVAVVFNLPE KVCQERNKNR TDRQVEEYVI RKHTQQLKKS IKGLQREGFR 150
    YVYILNSPEE VEEVVFERQP LWNNKKDEHG PFDIIGDIHG CYDELKMLLE 200
    KLGYLIEEVE GGVGSGKYRV THPEGRKVLF LGDLVDRGPK ITEVLKLVMG 250
    MVKSGIALCV PGNHDVKLLR KLNGRDVQIT HGLDRTLEQL AKEPQEFIEE 300
    VKAFIDGLVS HYVLDDGKLV VAHAGMKEEF QGRGSGKVRE FALYGETTGE 350
    TDEYGLPVRY DWASDYRGKA LVVYGHTPQA EVLKVNNTIN IDTGCVFGGK 400
    LTAYRYPERE IVDVKALKTY YEPAKPFLPK EDMAERFEAR TDNDILDIND 450
    VLGKKIITTR LMSSITIHEE NSIAALEVMS RFAADPHWLI YLPPTMSPCE 500
    TSKKEGMLEH PIEAFEYFRT RGVGKVVCEQ KHMGSRAVVI VCKDSQVAEK 550
    RFGVLDGTAG ICYTRTGRHF FDDMQLEAEL IDRVRKVLDK SGFWGDFNTD 600
    WVCLDCELMP WSAKAQKLLE EQYSAVGISG RVVLDEAVKL LKQASLNKTV 650
    SFDVSRQTSG KNADINELLQ RFTERSEMMQ KYVEAYRKYC WPVNSIDDLK 700
    LAPFHILATE GKVHSDKNHI WHMDTIAKYC TQDDSLIMAT NHILVDVTDA 750
    ESVDKGIKWW EDLTASGGEG MVVKPYDFIV KNGRELLQPA VKCRGREYLR 800
    IIYGPEYTMD ENIERLRNRA VGKKRSLALR EFSLGMEALE RFVRNEPLYR 850
    VHECVFGVLA LESEPVDPRL 870
    Length:870
    Mass (Da):98,776
    Last modified:March 20, 2007 - v1
    Checksum:i11CA5878DD2919E9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000568 Genomic DNA. Translation: ABN53967.1.
    RefSeqiWP_020457916.1. NC_009012.1.
    YP_001039160.1. NC_009012.1.

    Genome annotation databases

    EnsemblBacteriaiABN53967; ABN53967; Cthe_2768.
    GeneIDi4810085.
    KEGGicth:Cthe_2768.
    PATRICi19519588. VBICloThe47081_2947.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000568 Genomic DNA. Translation: ABN53967.1 .
    RefSeqi WP_020457916.1. NC_009012.1.
    YP_001039160.1. NC_009012.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3TY5 X-ray 2.40 A/B 479-870 [» ]
    3TY8 X-ray 2.60 A/B 479-870 [» ]
    3TY9 X-ray 3.12 A/B/C/D 479-870 [» ]
    4DRF X-ray 2.60 A/C 445-870 [» ]
    4E6N X-ray 2.39 A/C 445-870 [» ]
    4GP6 X-ray 2.10 A/B 1-170 [» ]
    4GP7 X-ray 2.00 A/B 1-170 [» ]
    4J6O X-ray 1.60 A/B 171-424 [» ]
    4JST X-ray 2.03 A/B 1-170 [» ]
    4JSY X-ray 2.14 A/B 1-170 [» ]
    4JT2 X-ray 2.49 A/B 1-170 [» ]
    4JT4 X-ray 2.01 A/B 1-170 [» ]
    4MDE X-ray 1.80 A/B 1-170 [» ]
    4MDF X-ray 1.73 A/B 1-170 [» ]
    ProteinModelPortali A3DJ38.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60067N.
    STRINGi 203119.Cthe_2768.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN53967 ; ABN53967 ; Cthe_2768 .
    GeneIDi 4810085.
    KEGGi cth:Cthe_2768.
    PATRICi 19519588. VBICloThe47081_2947.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000245786.
    KOi K01090.
    OMAi HGCRSEL.
    OrthoDBi EOG6WX4P8.

    Enzyme and pathway databases

    BioCyci CTHE203119:GIW8-2869-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR027417. P-loop_NTPase.
    IPR024028. PNKP_bac.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF56300. SSF56300. 1 hit.
    TIGRFAMsi TIGR04075. bacter_Pnkp. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372Imported.
    2. "The adenylyltransferase domain of bacterial Pnkp defines a unique RNA ligase family."
      Smith P., Wang L.K., Nair P.A., Shuman S.
      Proc. Natl. Acad. Sci. U.S.A. 109:2296-2301(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 479-870 IN COMPLEX WITH ADP; AMP; ATP AND MAGNESIUM.
    3. "Molecular basis of bacterial protein Hen1 activating the ligase activity of bacterial protein Pnkp for RNA repair."
      Wang P., Chan C.M., Christensen D., Zhang C., Selvadurai K., Huang R.H.
      Proc. Natl. Acad. Sci. U.S.A. 109:13248-13253(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.39 ANGSTROMS) OF 445-870 IN COMPLEX WITH AMP.
    4. "Structure and mechanism of the polynucleotide kinase component of the bacterial Pnkp-Hen1 RNA repair system."
      Wang L.K., Das U., Smith P., Shuman S.
      RNA 18:2277-2286(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 1-170 IN COMPLEX WITH ADP AND ATP.
    5. "Structural and biochemical analysis of the phosphate donor specificity of the polynucleotide kinase component of the bacterial pnkp.hen1 RNA repair system."
      Das U., Wang L.K., Smith P., Shuman S.
      Biochemistry 52:4734-4743(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) OF 1-170 IN COMPLEX WITH CTP AND GTP.
    6. "Structure and mechanism of the 2',3' phosphatase component of the bacterial Pnkp-Hen1 RNA repair system."
      Wang L.K., Smith P., Shuman S.
      Nucleic Acids Res. 41:5864-5873(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 171-424 IN COMPLEX WITH MANGANESE.
    7. "Structures of bacterial polynucleotide kinase in a Michaelis complex with GTP.Mg2+ and 5'-OH oligonucleotide and a product complex with GDP.Mg2+ and 5'-PO4 oligonucleotide reveal a mechanism of general acid-base catalysis and the determinants of phosphoacceptor recognition."
      Das U., Wang L.K., Smith P., Jacewicz A., Shuman S.
      Nucleic Acids Res. 42:1152-1161(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.73 ANGSTROMS) OF 1-170 IN COMPLEX WITH GTP.

    Entry informationi

    Entry nameiA3DJ38_CLOTH
    AccessioniPrimary (citable) accession number: A3DJ38
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 20, 2007
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3