A3DIZ9 (EFG_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	Cthe_2729

Organism

Clostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]

Taxonomic identifier

203119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	697 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 697	697	Elongation factor G HAMAP MF_00054_B		PRO_1000008817
Regions
	Nucleotide binding	17 – 24	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	135 – 138	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3DIZ9 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 014A4515745F05F7	FASTA	697	77,636
	10 20 30 40 50 60 MPRQFSLENT RNIGIMAHID AGKTTTTERI LFYTGRVHKI GETHEGSATM DWMEQEQERG 70 80 90 100 110 120 ITITSAATTA QWKGTRINII DTPGHVDFTV EVERSLRVLD GAVAVFCAKG GVEPQSETVW 130 140 150 160 170 180 RQADKYKVPR MAYVNKMDIM GADFFNCIKM MKERLQANPV PIQLPIGKED NFQGIIDLIE 190 200 210 220 230 240 MKAYYYMDDL GKVIEQRDIP EDMRELAEEY RTNLLENVAE YDEELMMKYL EGEEITEAEI 250 260 270 280 290 300 KAALRKGTIA VKAIPVLCGS SYKNKGVQRL LDAIVDYMPS PVDIEAIKGV SVDGETEIER 310 320 330 340 350 360 HASDDEPFSA LAFKIMSDPY VGKLCFFRVY SGKLSSGSYV LNATKGKRER IGRLLMMHAN 370 380 390 400 410 420 HREEVDMVYA GDIAAAVGLK ETTTGDTLCD EANPVILESM NFPEPVIHVA IEPKTKAGQE 430 440 450 460 470 480 KMALALQKLA EEDPTFRTYT DQETGQTIIA GMGELHLEII VDRLLREFKV EANVGNPQVA 490 500 510 520 530 540 YKETIRKSVK SEGKYIRQSG GKGQYGHCWI EIEPKERGTG YEFVNKIVGG VIPKEYIPAV 550 560 570 580 590 600 DAGIQSAMNN GVLAGYPVVD VKVTLYDGSY HEVDSSEMAF KVAASMAFKE GMKKADPVIL 610 620 630 640 650 660 EPIMKVVVTV PEDYMGDVIG DLNSRRGRIE GMEARAGAQV IHAYVPLAEM FGYATALRSR 670 680 690 SQGRGVFSME ISHFEEVPKN IQEQIISGRA KNNSSDE

References

[1]

"Complete sequence of Clostridium thermocellum ATCC 27405." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27405 / DSM 1237.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000568 Genomic DNA. Translation: ABN53928.1.
RefSeq	YP_001039121.1. NC_009012.1.
3D structure databases
ProteinModelPortal	A3DIZ9.
SMR	A3DIZ9. Positions 4-686.
ModBase	Search...
Protein-protein interaction databases
STRING	A3DIZ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4810231.
GenomeReviews	Gene locus Cthe_2729 in contig CP000568_GR.
KEGG	cth:Cthe_2729.
NMPDR	fig|203119.1.peg.3219.
PATRIC	19519500. VBICloThe47081_2903.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0480.
HOGENOM	HBG737692.
OMA	CNEWREK.
ProtClustDB	PRK00007.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_CLOTH

Accession

Primary (citable) accession number: A3DIZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 20, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program