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Protein

Glutamate-1-semialdehyde 2,1-aminomutase

Gene

hemL

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-4-amino-5-oxopentanoate = 5-aminolevulinate.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamyl-tRNA reductase (hemA)
  2. Glutamate-1-semialdehyde 2,1-aminomutase (hemL)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processPorphyrin biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciCTHE203119:G1G86-2650-MONOMER
UniPathwayiUPA00251; UER00317

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutaseUniRule annotation (EC:5.4.3.8UniRule annotation)
Short name:
GSAUniRule annotation
Alternative name(s):
Glutamate-1-semialdehyde aminotransferaseUniRule annotation
Short name:
GSA-ATUniRule annotation
Gene namesi
Name:hemLUniRule annotation
Ordered Locus Names:Cthe_2530
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000599841 – 427Glutamate-1-semialdehyde 2,1-aminomutaseAdd BLAST427

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei267N6-(pyridoxal phosphate)lysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi203119.Cthe_2530

Structurei

3D structure databases

ProteinModelPortaliA3DIF3
SMRiA3DIF3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDM Bacteria
COG0001 LUCA
HOGENOMiHOG000020210
KOiK01845
OMAiWGPLIFG
OrthoDBiPOG091H04O1

Family and domain databases

CDDicd00610 OAT_like, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
HAMAPiMF_00375 HemL_aminotrans_3, 1 hit
InterProiView protein in InterPro
IPR004639 4pyrrol_synth_GluAld_NH2Trfase
IPR005814 Aminotrans_3
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00202 Aminotran_3, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR00713 hemL, 1 hit
PROSITEiView protein in PROSITE
PS00600 AA_TRANSFER_CLASS_3, 1 hit

Sequencei

Sequence statusi: Complete.

A3DIF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLSKSKLLF ERAKKVIPGG VNSPVRAFGA VGGYPPFIKK AKGARIYDAD
60 70 80 90 100
GNEYIDYVGS WGPMILGHSH PRVLEAVSKT MVDGLSFGAA TELEVQMAEL
110 120 130 140 150
ITELVPSVEM VRMVNSGTEA VMSAIRVARG YTKREKIIKF AGCYHGHADS
160 170 180 190 200
MLVKVGSGAM TNGIPNSGGV TAGAAKDTLI ARYNDIDSVK LLFEQNKGNI
210 220 230 240 250
AAVIVEPVAA NMGVVPPKDN FLEELRKLCD KEEALLIFDE VITGFRLAIG
260 270 280 290 300
GAQQYFGVNA DLVTYGKIIG GGMPVGAYGG RREIMECVAP VGDVYQAGTL
310 320 330 340 350
SGNPIAMSAG IATLRELYEN PGIFENINRL GQRLSEGLGK ITKYTVKAVG
360 370 380 390 400
SLVCVFMTEE EVNDYDSAVK SDTGLFGRYF NHLLNNGIYI APSQFEAMFV
410 420
SNAHTDKDID ETLEKVSLFF ARKSPPA
Length:427
Mass (Da):45,932
Last modified:March 20, 2007 - v1
Checksum:iA91AAE58A0A0914C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA Translation: ABN53732.1
RefSeqiWP_003513104.1, NC_009012.1

Genome annotation databases

EnsemblBacteriaiABN53732; ABN53732; Cthe_2530
GeneIDi35803579
KEGGicth:Cthe_2530

Similar proteinsi

Entry informationi

Entry nameiGSA_CLOTH
AccessioniPrimary (citable) accession number: A3DIF3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health