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A3DI22 (FTHS_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:Cthe_2399
OrganismClostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]
Taxonomic identifier203119 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_0000300522

Regions

Nucleotide binding65 – 728ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A3DI22 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: 69D406E74CC79253

FASTA55660,199
        10         20         30         40         50         60 
MLTDIQIAQS CKMKPITQVA AELGIDEEEL ELYGKYKAKL SDKLWERVKD RPDGKLVLVT 

        70         80         90        100        110        120 
AINPTPAGEG KTTTTVGLGQ AMARIGKKAV IALREPSLGP VMGIKGGAAG GGYSQVVPME 

       130        140        150        160        170        180 
DINLHFTGDM HAITAANNLL SAAIDNHIQQ GNELNIDVRQ IIWKRAMDMN DRALRNIVVG 

       190        200        210        220        230        240 
LGGKANGVPR EDGFQITVAS EVMAVLCLST GLMDLKERLG RILIGYTYDG KPVFAKDLKV 

       250        260        270        280        290        300 
NGAMALLLKD AIKPNLVQTL ENTPAIVHGG PFANIAHGCN SIVATRLGLK LADYCITEAG 

       310        320        330        340        350        360 
FGADLGAEKF FNIKCRYAGL KPDLVVLVAT IRALKYNGGV KKENLGIENL PALEKGFVNL 

       370        380        390        400        410        420 
EKHIENIRKF QVPLLVAINH FDTDSEAEIE YVKNRCKALN VEVAFSDVFS KGSEGGIELA 

       430        440        450        460        470        480 
EKVVKLTETQ KSNFKPLYDV NLSIREKIEI IAREIYGADS VNILPAAERA IKKIEELKMD 

       490        500        510        520        530        540 
KLPICVAKTQ YSLSDDPTLL GRPQGFVITV REIKLSSGAG FIVAITGDIM TMPGLPKVPA 

       550 
AEKIDIDENG VITGLF 

« Hide

References

[1]"Complete sequence of Clostridium thermocellum ATCC 27405."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27405 / DSM 1237.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000568 Genomic DNA. Translation: ABN53601.1.
RefSeqYP_001038794.1. NC_009012.1.

3D structure databases

ProteinModelPortalA3DI22.
SMRA3DI22. Positions 4-554.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203119.Cthe_2399.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN53601; ABN53601; Cthe_2399.
GeneID4811051.
KEGGcth:Cthe_2399.
PATRIC19518782. VBICloThe47081_2553.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMALKHHGGV.
OrthoDBEOG6PCPSP.

Enzyme and pathway databases

BioCycCTHE203119:GIW8-2476-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_CLOTH
AccessionPrimary (citable) accession number: A3DI22
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 20, 2007
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways