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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathway: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-2476-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:Cthe_2399
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
ProteomesiUP000002145 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Formate--tetrahydrofolate ligasePRO_0000300522Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_2399.

Structurei

3D structure databases

ProteinModelPortaliA3DI22.
SMRiA3DI22. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3DI22-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTDIQIAQS CKMKPITQVA AELGIDEEEL ELYGKYKAKL SDKLWERVKD
60 70 80 90 100
RPDGKLVLVT AINPTPAGEG KTTTTVGLGQ AMARIGKKAV IALREPSLGP
110 120 130 140 150
VMGIKGGAAG GGYSQVVPME DINLHFTGDM HAITAANNLL SAAIDNHIQQ
160 170 180 190 200
GNELNIDVRQ IIWKRAMDMN DRALRNIVVG LGGKANGVPR EDGFQITVAS
210 220 230 240 250
EVMAVLCLST GLMDLKERLG RILIGYTYDG KPVFAKDLKV NGAMALLLKD
260 270 280 290 300
AIKPNLVQTL ENTPAIVHGG PFANIAHGCN SIVATRLGLK LADYCITEAG
310 320 330 340 350
FGADLGAEKF FNIKCRYAGL KPDLVVLVAT IRALKYNGGV KKENLGIENL
360 370 380 390 400
PALEKGFVNL EKHIENIRKF QVPLLVAINH FDTDSEAEIE YVKNRCKALN
410 420 430 440 450
VEVAFSDVFS KGSEGGIELA EKVVKLTETQ KSNFKPLYDV NLSIREKIEI
460 470 480 490 500
IAREIYGADS VNILPAAERA IKKIEELKMD KLPICVAKTQ YSLSDDPTLL
510 520 530 540 550
GRPQGFVITV REIKLSSGAG FIVAITGDIM TMPGLPKVPA AEKIDIDENG

VITGLF
Length:556
Mass (Da):60,199
Last modified:March 20, 2007 - v1
Checksum:i69D406E74CC79253
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA. Translation: ABN53601.1.
RefSeqiWP_003516448.1. NC_009012.1.
YP_001038794.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN53601; ABN53601; Cthe_2399.
KEGGicth:Cthe_2399.
PATRICi19518782. VBICloThe47081_2553.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA. Translation: ABN53601.1.
RefSeqiWP_003516448.1. NC_009012.1.
YP_001038794.1. NC_009012.1.

3D structure databases

ProteinModelPortaliA3DI22.
SMRiA3DI22. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203119.Cthe_2399.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN53601; ABN53601; Cthe_2399.
KEGGicth:Cthe_2399.
PATRICi19518782. VBICloThe47081_2553.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciCTHE203119:GIW8-2476-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

Entry informationi

Entry nameiFTHS_CLOTH
AccessioniPrimary (citable) accession number: A3DI22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 20, 2007
Last modified: May 27, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.