A3DH67 (GUNS_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase SS
Short name=EGSS
EC=3.2.1.4

Alternative name(s):
Cellulase SS
Endo-1,4-beta-glucanase

Gene names

Name:	celS
Ordered Locus Names:	Cthe_2089

Organism

Clostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]

Taxonomic identifier

203119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	741 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted.
Domain	A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.
Sequence similarities	Belongs to the glycosyl hydrolase 48 (cellulase L) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Ref.1
	Chain	28 – 741	714	Endoglucanase SS		PRO_0000284723
Regions
	Repeat	679 – 702	24	1
	Repeat	711 – 734	24	2
	Region	679 – 734	56	2 X 24 AA approximate repeats

Sequences

	Sequence		Length	Mass (Da)
	A3DH67 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 39FDF4680BDB1144	FASTA	741	83,558
	10 20 30 40 50 60 MVKSRKISIL LAVAMLVSIM IPTTAFAGPT KAPTKDGTSY KDLFLELYGK IKDPKNGYFS 70 80 90 100 110 120 PDEGIPYHSI ETLIVEAPDY GHVTTSEAFS YYVWLEAMYG NLTGNWSGVE TAWKVMEDWI 130 140 150 160 170 180 IPDSTEQPGM SSYNPNSPAT YADEYEDPSY YPSELKFDTV RVGSDPVHND LVSAYGPNMY 190 200 210 220 230 240 LMHWLMDVDN WYGFGTGTRA TFINTFQRGE QESTWETIPH PSIEEFKYGG PNGFLDLFTK 250 260 270 280 290 300 DRSYAKQWRY TNAPDAEGRA IQAVYWANKW AKEQGKGSAV ASVVSKAAKM GDFLRNDMFD 310 320 330 340 350 360 KYFMKIGAQD KTPATGYDSA HYLMAWYTAW GGGIGASWAW KIGCSHAHFG YQNPFQGWVS 370 380 390 400 410 420 ATQSDFAPKS SNGKRDWTTS YKRQLEFYQW LQSAEGGIAG GATNSWNGRY EKYPAGTSTF 430 440 450 460 470 480 YGMAYVPHPV YADPGSNQWF GFQAWSMQRV MEYYLETGDS SVKNLIKKWV DWVMSEIKLY 490 500 510 520 530 540 DDGTFAIPSD LEWSGQPDTW TGTYTGNPNL HVRVTSYGTD LGVAGSLANA LATYAAATER 550 560 570 580 590 600 WEGKLDTKAR DMAAELVNRA WYNFYCSEGK GVVTEEARAD YKRFFEQEVY VPAGWSGTMP 610 620 630 640 650 660 NGDKIQPGIK FIDIRTKYRQ DPYYDIVYQA YLRGEAPVLN YHRFWHEVDL AVAMGVLATY 670 680 690 700 710 720 FPDMTYKVPG TPSTKLYGDV NDDGKVNSTD AVALKRYVLR SGISINTDNA DLNEDGRVNS 730 740 TDLGILKRYI LKEIDTLPYK N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and DNA sequence of the gene coding for Clostridium thermocellum cellulase Ss (CelS), a major cellulosome component." Wang W.K., Kruus K., Wu J.H.D. J. Bacteriol. 175:1293-1302(1993) [PubMed: 8444792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-37; 412-431 AND 589-605.
[2]	"Product inhibition of the recombinant CelS, an exoglucanase component of the Clostridium thermocellum cellulosome." Kruus K., Andreacchi A., Wang W.K., Wu J.H.D. Appl. Microbiol. Biotechnol. 44:399-404(1995) [PubMed: 8597541] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete sequence of Clostridium thermocellum ATCC 27405." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27405 / DSM 1237.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L06942 Genomic DNA. Translation: AAA23226.1. CP000568 Genomic DNA. Translation: ABN53296.1.
PIR	A47063.
RefSeq	YP_001038489.1. NC_009012.1.
3D structure databases
ProteinModelPortal	A3DH67.
SMR	A3DH67. Positions 28-669, 673-741.
ModBase	Search...
Protein-protein interaction databases
STRING	A3DH67.
Protein family/group databases
CAZy	GH48. Glycoside Hydrolase Family 48.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4810949.
GenomeReviews	Gene locus Cthe_2089 in contig CP000568_GR.
KEGG	cth:Cthe_2089.
NMPDR	fig|203119.1.peg.248.
PATRIC	19518092. VBICloThe47081_2217.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG10655.
HOGENOM	HBG345030.
OMA	GMHWLLD.
ProtClustDB	CLSK801496.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16423.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR016134. Cellulos_enz_dockerin_1. IPR002105. Cellulos_enz_dockerin_1_Ca-bd. IPR018242. Dockerin_1. IPR000556. Glyco_hydro_48F. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 4 hits. G3DSA:1.10.1330.10. Cellulos_enz_dockerin_1. 1 hit.
Pfam	PF00404. Dockerin_1. 2 hits. PF02011. Glyco_hydro_48. 1 hit. [Graphical view]
PRINTS	PR00844. GLHYDRLASE48.
SUPFAM	SSF63446. Cellulos_enz_dockerin_1. 1 hit. SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00448. CLOS_CELLULOSOME_RPT. 2 hits. PS00018. EF_HAND_1. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNS_CLOTH

Accession

Primary (citable) accession number: A3DH67
Secondary accession number(s): P38686

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	March 20, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program