Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cellulose 1,4-beta-cellobiosidase (reducing end) CelS

Gene

celS

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme catalyzes the exohydrolysis of 1,4-beta-glucosidic linkages in cellulose with a preference for amorphous or crystalline cellulose over carboxymethyl cellulose.1 Publication

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and similar substrates, releasing cellobiose from the reducing ends of the chains.1 Publication

Enzyme regulationi

Inhibited by cellobiose and lactose, but not by glucose.1 Publication

pH dependencei

Optimum pH is 5-6.1 Publication

Temperature dependencei

Optimum temperature is 70 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei76SubstrateBy similarity1
Active sitei87Proton donorBy similarity1
Binding sitei140SubstrateBy similarity1
Binding sitei204SubstrateBy similarity1
Binding sitei241SubstrateBy similarity1
Binding sitei247SubstrateBy similarity1
Active sitei255NucleophileBy similarity1
Binding sitei421SubstrateBy similarity1
Binding sitei520SubstrateBy similarity1
Metal bindingi679Calcium 1By similarity1
Metal bindingi681Calcium 1By similarity1
Metal bindingi683Calcium 1By similarity1
Metal bindingi684Calcium 1; via amide nitrogenBy similarity1
Metal bindingi685Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi690Calcium 1By similarity1
Metal bindingi711Calcium 2By similarity1
Metal bindingi711Calcium 3By similarity1
Metal bindingi712Calcium 2; via amide nitrogenBy similarity1
Metal bindingi713Calcium 3By similarity1
Metal bindingi715Calcium 3By similarity1
Metal bindingi717Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi717Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi722Calcium 2By similarity1
Metal bindingi722Calcium 3By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16423
BRENDAi3.2.1.176 1530

Protein family/group databases

CAZyiGH48 Glycoside Hydrolase Family 48

Names & Taxonomyi

Protein namesi
Recommended name:
Cellulose 1,4-beta-cellobiosidase (reducing end) CelS (EC:3.2.1.176)
Alternative name(s):
Cellobiohydrolase CelS
Cellulase SS
Endo-1,4-beta-glucanase
Endoglucanase SS
Short name:
EGSS
Exocellulase
Gene namesi
Name:celS
Ordered Locus Names:Cthe_2089
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
ChainiPRO_000028472328 – 741Cellulose 1,4-beta-cellobiosidase (reducing end) CelSAdd BLAST714

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_2089

Structurei

3D structure databases

ProteinModelPortaliA3DH67
SMRiA3DH67
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini673 – 739DockerinPROSITE-ProRule annotationAdd BLAST67

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni251 – 252Substrate bindingBy similarity2
Regioni301 – 302Substrate bindingBy similarity2
Regioni326 – 327Substrate bindingBy similarity2
Regioni645 – 646Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CYP Bacteria
ENOG410XPC9 LUCA
HOGENOMiHOG000020369
KOiK20829
OMAiKYTNAPD
OrthoDBiPOG091H0EGP

Family and domain databases

Gene3Di1.50.10.10, 5 hits
2.170.160.10, 3 hits
4.10.870.10, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR002105 Dockerin_1_rpt
IPR016134 Dockerin_dom
IPR036439 Dockerin_dom_sf
IPR023309 Endo-1-4-beta-glucanase_dom2
IPR027390 Endoglucanase_F_dom3
IPR000556 Glyco_hydro_48F
PfamiView protein in Pfam
PF00404 Dockerin_1, 2 hits
PF02011 Glyco_hydro_48, 1 hit
PRINTSiPR00844 GLHYDRLASE48
SUPFAMiSSF48208 SSF48208, 1 hit
SSF63446 SSF63446, 1 hit
PROSITEiView protein in PROSITE
PS00448 CLOS_CELLULOSOME_RPT, 2 hits
PS51766 DOCKERIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A3DH67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKSRKISIL LAVAMLVSIM IPTTAFAGPT KAPTKDGTSY KDLFLELYGK
60 70 80 90 100
IKDPKNGYFS PDEGIPYHSI ETLIVEAPDY GHVTTSEAFS YYVWLEAMYG
110 120 130 140 150
NLTGNWSGVE TAWKVMEDWI IPDSTEQPGM SSYNPNSPAT YADEYEDPSY
160 170 180 190 200
YPSELKFDTV RVGSDPVHND LVSAYGPNMY LMHWLMDVDN WYGFGTGTRA
210 220 230 240 250
TFINTFQRGE QESTWETIPH PSIEEFKYGG PNGFLDLFTK DRSYAKQWRY
260 270 280 290 300
TNAPDAEGRA IQAVYWANKW AKEQGKGSAV ASVVSKAAKM GDFLRNDMFD
310 320 330 340 350
KYFMKIGAQD KTPATGYDSA HYLMAWYTAW GGGIGASWAW KIGCSHAHFG
360 370 380 390 400
YQNPFQGWVS ATQSDFAPKS SNGKRDWTTS YKRQLEFYQW LQSAEGGIAG
410 420 430 440 450
GATNSWNGRY EKYPAGTSTF YGMAYVPHPV YADPGSNQWF GFQAWSMQRV
460 470 480 490 500
MEYYLETGDS SVKNLIKKWV DWVMSEIKLY DDGTFAIPSD LEWSGQPDTW
510 520 530 540 550
TGTYTGNPNL HVRVTSYGTD LGVAGSLANA LATYAAATER WEGKLDTKAR
560 570 580 590 600
DMAAELVNRA WYNFYCSEGK GVVTEEARAD YKRFFEQEVY VPAGWSGTMP
610 620 630 640 650
NGDKIQPGIK FIDIRTKYRQ DPYYDIVYQA YLRGEAPVLN YHRFWHEVDL
660 670 680 690 700
AVAMGVLATY FPDMTYKVPG TPSTKLYGDV NDDGKVNSTD AVALKRYVLR
710 720 730 740
SGISINTDNA DLNEDGRVNS TDLGILKRYI LKEIDTLPYK N
Length:741
Mass (Da):83,558
Last modified:March 20, 2007 - v1
Checksum:i39FDF4680BDB1144
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06942 Genomic DNA Translation: AAA23226.1
CP000568 Genomic DNA Translation: ABN53296.1
PIRiA47063

Genome annotation databases

EnsemblBacteriaiABN53296; ABN53296; Cthe_2089
KEGGicth:Cthe_2089

Similar proteinsi

Entry informationi

Entry nameiGUNS_CLOTH
AccessioniPrimary (citable) accession number: A3DH67
Secondary accession number(s): P38686
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: March 20, 2007
Last modified: March 28, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health