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A3DH67

- GUNS_CLOTH

UniProt

A3DH67 - GUNS_CLOTH

Protein

Cellulose 1,4-beta-cellobiosidase (reducing end) CelS

Gene

celS

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (20 Mar 2007)
      Previous versions | rss
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    Functioni

    This enzyme catalyzes the exohydrolysis of 1,4-beta-glucosidic linkages in cellulose with a preference for amorphous or crystalline cellulose over carboxymethyl cellulose.1 Publication

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and similar substrates, releasing cellobiose from the reducing ends of the chains.1 Publication

    Enzyme regulationi

    Inhibited by cellobiose and lactose, but not by glucose.1 Publication

    pH dependencei

    Optimum pH is 5-6.1 Publication

    Temperature dependencei

    Optimum temperature is 70 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei76 – 761SubstrateBy similarity
    Active sitei87 – 871Proton donorBy similarity
    Binding sitei140 – 1401SubstrateBy similarity
    Binding sitei204 – 2041SubstrateBy similarity
    Binding sitei241 – 2411SubstrateBy similarity
    Binding sitei247 – 2471SubstrateBy similarity
    Active sitei255 – 2551NucleophileBy similarity
    Binding sitei421 – 4211SubstrateBy similarity
    Binding sitei520 – 5201SubstrateBy similarity
    Metal bindingi679 – 6791Calcium 1By similarity
    Metal bindingi681 – 6811Calcium 1By similarity
    Metal bindingi683 – 6831Calcium 1By similarity
    Metal bindingi684 – 6841Calcium 1; via amide nitrogenBy similarity
    Metal bindingi685 – 6851Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi690 – 6901Calcium 1By similarity
    Metal bindingi711 – 7111Calcium 2By similarity
    Metal bindingi711 – 7111Calcium 3By similarity
    Metal bindingi712 – 7121Calcium 2; via amide nitrogenBy similarity
    Metal bindingi713 – 7131Calcium 3By similarity
    Metal bindingi715 – 7151Calcium 3By similarity
    Metal bindingi717 – 7171Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi717 – 7171Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi722 – 7221Calcium 2By similarity
    Metal bindingi722 – 7221Calcium 3By similarity

    GO - Molecular functioni

    1. cellulase activity Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BioCyciCTHE203119:GIW8-2150-MONOMER.
    MetaCyc:MONOMER-16423.

    Protein family/group databases

    CAZyiGH48. Glycoside Hydrolase Family 48.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cellulose 1,4-beta-cellobiosidase (reducing end) CelS (EC:3.2.1.176)
    Alternative name(s):
    Cellobiohydrolase CelS
    Cellulase SS
    Endo-1,4-beta-glucanase
    Endoglucanase SS
    Short name:
    EGSS
    Exocellulase
    Gene namesi
    Name:celS
    Ordered Locus Names:Cthe_2089
    OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
    Taxonomic identifieri203119 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
    ProteomesiUP000002145: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 27271 PublicationAdd
    BLAST
    Chaini28 – 741714Cellulose 1,4-beta-cellobiosidase (reducing end) CelSPRO_0000284723Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi203119.Cthe_2089.

    Structurei

    3D structure databases

    ProteinModelPortaliA3DH67.
    SMRiA3DH67. Positions 28-669, 673-741.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini679 – 69921Dockerin 1Add
    BLAST
    Domaini711 – 73121Dockerin 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni251 – 2522Substrate bindingBy similarity
    Regioni301 – 3022Substrate bindingBy similarity
    Regioni326 – 3272Substrate bindingBy similarity
    Regioni645 – 6462Substrate bindingBy similarity

    Domaini

    The dockerin domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

    Sequence similaritiesi

    Contains 2 dockerin domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG10655.
    HOGENOMiHOG000020369.
    OMAiTYHRFWS.
    OrthoDBiEOG64V28H.

    Family and domain databases

    Gene3Di1.10.1330.10. 1 hit.
    1.50.10.10. 4 hits.
    4.10.870.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR018242. Dockerin_1.
    IPR027390. Endoglucanase_F_dom3.
    IPR000556. Glyco_hydro_48F.
    [Graphical view]
    PfamiPF00404. Dockerin_1. 2 hits.
    PF02011. Glyco_hydro_48. 1 hit.
    [Graphical view]
    PRINTSiPR00844. GLHYDRLASE48.
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A3DH67-1 [UniParc]FASTAAdd to Basket

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    MVKSRKISIL LAVAMLVSIM IPTTAFAGPT KAPTKDGTSY KDLFLELYGK    50
    IKDPKNGYFS PDEGIPYHSI ETLIVEAPDY GHVTTSEAFS YYVWLEAMYG 100
    NLTGNWSGVE TAWKVMEDWI IPDSTEQPGM SSYNPNSPAT YADEYEDPSY 150
    YPSELKFDTV RVGSDPVHND LVSAYGPNMY LMHWLMDVDN WYGFGTGTRA 200
    TFINTFQRGE QESTWETIPH PSIEEFKYGG PNGFLDLFTK DRSYAKQWRY 250
    TNAPDAEGRA IQAVYWANKW AKEQGKGSAV ASVVSKAAKM GDFLRNDMFD 300
    KYFMKIGAQD KTPATGYDSA HYLMAWYTAW GGGIGASWAW KIGCSHAHFG 350
    YQNPFQGWVS ATQSDFAPKS SNGKRDWTTS YKRQLEFYQW LQSAEGGIAG 400
    GATNSWNGRY EKYPAGTSTF YGMAYVPHPV YADPGSNQWF GFQAWSMQRV 450
    MEYYLETGDS SVKNLIKKWV DWVMSEIKLY DDGTFAIPSD LEWSGQPDTW 500
    TGTYTGNPNL HVRVTSYGTD LGVAGSLANA LATYAAATER WEGKLDTKAR 550
    DMAAELVNRA WYNFYCSEGK GVVTEEARAD YKRFFEQEVY VPAGWSGTMP 600
    NGDKIQPGIK FIDIRTKYRQ DPYYDIVYQA YLRGEAPVLN YHRFWHEVDL 650
    AVAMGVLATY FPDMTYKVPG TPSTKLYGDV NDDGKVNSTD AVALKRYVLR 700
    SGISINTDNA DLNEDGRVNS TDLGILKRYI LKEIDTLPYK N 741
    Length:741
    Mass (Da):83,558
    Last modified:March 20, 2007 - v1
    Checksum:i39FDF4680BDB1144
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06942 Genomic DNA. Translation: AAA23226.1.
    CP000568 Genomic DNA. Translation: ABN53296.1.
    PIRiA47063.
    RefSeqiWP_020457708.1. NC_009012.1.
    YP_001038489.1. NC_009012.1.

    Genome annotation databases

    EnsemblBacteriaiABN53296; ABN53296; Cthe_2089.
    GeneIDi4810949.
    KEGGicth:Cthe_2089.
    PATRICi19518092. VBICloThe47081_2217.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06942 Genomic DNA. Translation: AAA23226.1 .
    CP000568 Genomic DNA. Translation: ABN53296.1 .
    PIRi A47063.
    RefSeqi WP_020457708.1. NC_009012.1.
    YP_001038489.1. NC_009012.1.

    3D structure databases

    ProteinModelPortali A3DH67.
    SMRi A3DH67. Positions 28-669, 673-741.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 203119.Cthe_2089.

    Protein family/group databases

    CAZyi GH48. Glycoside Hydrolase Family 48.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN53296 ; ABN53296 ; Cthe_2089 .
    GeneIDi 4810949.
    KEGGi cth:Cthe_2089.
    PATRICi 19518092. VBICloThe47081_2217.

    Phylogenomic databases

    eggNOGi NOG10655.
    HOGENOMi HOG000020369.
    OMAi TYHRFWS.
    OrthoDBi EOG64V28H.

    Enzyme and pathway databases

    BioCyci CTHE203119:GIW8-2150-MONOMER.
    MetaCyc:MONOMER-16423.

    Family and domain databases

    Gene3Di 1.10.1330.10. 1 hit.
    1.50.10.10. 4 hits.
    4.10.870.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR018242. Dockerin_1.
    IPR027390. Endoglucanase_F_dom3.
    IPR000556. Glyco_hydro_48F.
    [Graphical view ]
    Pfami PF00404. Dockerin_1. 2 hits.
    PF02011. Glyco_hydro_48. 1 hit.
    [Graphical view ]
    PRINTSi PR00844. GLHYDRLASE48.
    SUPFAMi SSF48208. SSF48208. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and DNA sequence of the gene coding for Clostridium thermocellum cellulase Ss (CelS), a major cellulosome component."
      Wang W.K., Kruus K., Wu J.H.D.
      J. Bacteriol. 175:1293-1302(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-37; 412-431 AND 589-605.
    2. "Product inhibition of the recombinant CelS, an exoglucanase component of the Clostridium thermocellum cellulosome."
      Kruus K., Andreacchi A., Wang W.K., Wu J.H.D.
      Appl. Microbiol. Biotechnol. 44:399-404(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME REGULATION.
    3. "Exoglucanase activities of the recombinant Clostridium thermocellum CelS, a major cellulosome component."
      Kruus K., Wang W.K., Ching J., Wu J.H.
      J. Bacteriol. 177:1641-1644(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

    Entry informationi

    Entry nameiGUNS_CLOTH
    AccessioniPrimary (citable) accession number: A3DH67
    Secondary accession number(s): P38686
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 17, 2007
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3