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A3DGS7 (DDL_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Cthe_1938
OrganismClostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]
Taxonomic identifier203119 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length376 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 376376D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341083

Regions

Domain155 – 361207ATP-grasp
Nucleotide binding188 – 24356ATP By similarity

Sites

Metal binding3141Magnesium or manganese 1 By similarity
Metal binding3281Magnesium or manganese 1 By similarity
Metal binding3281Magnesium or manganese 2 By similarity
Metal binding3301Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A3DGS7 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: 5E7BF27ABC8B31A6

FASTA37641,923
        10         20         30         40         50         60 
MGDKKRVLVI FGGQSSEHEV SRISATSILK NINLDKFDVS MIGITKDGKW LYYDGPIDKI 

        70         80         90        100        110        120 
PSGEWEEIAL KDGTRSIADR VSLFDNIISC KNNACGLEKA SENEKSKKID VVFPVLHGCN 

       130        140        150        160        170        180 
GEDGTIQGLF ELAGIPYVGC GVLASAVGMD KIYAKIIFEK AGIPQADYLY FTRKEIYGDV 

       190        200        210        220        230        240 
EGVVDKIEEK FSYPVFVKPS NAGSSVGVSK AHDKNELKEA LIYAARYDRK VLIEEFINGR 

       250        260        270        280        290        300 
EVECAVLGND DPVASTVGEI IPGNEFYDYK AKYIENTSKI KIPADLPEET VEQIRNYAVK 

       310        320        330        340        350        360 
AFKALDCSGL ARVDFFVHKE TGKVYINEIN TMPGFTSISM YPMLWEESGI SYPELIEKLI 

       370 
DLAVQRYNDN LKEYDE 

« Hide

References

[1]"Complete sequence of Clostridium thermocellum ATCC 27405."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27405 / DSM 1237.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000568 Genomic DNA. Translation: ABN53156.1.
RefSeqYP_001038349.1. NC_009012.1.

3D structure databases

ProteinModelPortalA3DGS7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203119.Cthe_1938.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN53156; ABN53156; Cthe_1938.
GeneID4810796.
KEGGcth:Cthe_1938.
PATRIC19517759. VBICloThe47081_2053.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAKLAFQYD.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycCTHE203119:GIW8-2001-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_CLOTH
AccessionPrimary (citable) accession number: A3DGS7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 20, 2007
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways