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A3DEU0 (SYL_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Cthe_1237
OrganismClostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]
Taxonomic identifier203119 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length825 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 825825Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009331

Regions

Motif40 – 5011"HIGH" region HAMAP-Rule MF_00049
Motif580 – 5845"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5831ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A3DEU0 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: 45416BD31E0D0AEC

FASTA82594,886
        10         20         30         40         50         60 
MYYNFVDIEK KWQKKWLEEK AFAVREDESK KKYYVLEMFP YPSGNLHMGH VRNYSIGDVV 

        70         80         90        100        110        120 
ARFKRMNGFN VLHPMGWDAF GLPAENAAIK RGVHPNDWTW SNIDNMRRQL KQLGISYDWD 

       130        140        150        160        170        180 
REVATCHPDY YKWTQWMFLQ LYKNGLAYKK KAYVNWCPSC ATVLANEQVV NGVCERCKSV 

       190        200        210        220        230        240 
VGKKDLEQWF FKITDYAQRL LDDIEKLKGW PDKVKVMQQN WIGRSEGVEV DFKVDGMDKA 

       250        260        270        280        290        300 
VRVYTTRPDT IYGVTYVVIA PEHPVVKELI KGTEQEQVCN EFINKMMFLN EIDRTATDVE 

       310        320        330        340        350        360 
KEGVFTGRYV INPLNGDRVP LYLANYVLAE YGTGVVMAVP AHDQRDFEFA KKYNLPIKVV 

       370        380        390        400        410        420 
IQPEGQELDA SRMTEAFVEV GYLVNSAEFD GVRSDEAIGK IIDYIEQKGY GKRKINYRLR 

       430        440        450        460        470        480 
DWLISRQRYW GAPIPIIYCD DCGAVPVPEE DLPVILPTDI KFSGVGESPL STSETFISAP 

       490        500        510        520        530        540 
CPKCGKMGRR ELDTMDTFVC SSWYYLRYCD PCNDKAPFDK ERIRYWLPVD QYIGGVEHAI 

       550        560        570        580        590        600 
LHLLYSRFLM KVLYDLGYVD YDEPFTNLLT QGMVLKDGAK MSKSLGNVVS PEEIIEKYGA 

       610        620        630        640        650        660 
DTARLFILFA SPPEKDLEWS DQGVEGCYRF INRVWRIVNE FADAVKEGGN IDTSTFTKAD 

       670        680        690        700        710        720 
KELWYMLNNT LKRVTDDISQ RFNFNTAISA VMELVNSLYY YKDKVADDSK NKALVREVIE 

       730        740        750        760        770        780 
KLIIMLAPFI PHATEELWSA IGKEGSVHEQ KWPSFDPAAL VKDEIEIVVQ INGKVRDKIV 

       790        800        810        820 
VPSDLTKEQV EERALNSEKI KAETAGKNVV KVISVPGKLV NIVVK 

« Hide

References

[1]"Complete sequence of Clostridium thermocellum ATCC 27405."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27405 / DSM 1237.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000568 Genomic DNA. Translation: ABN52469.1.
RefSeqYP_001037662.1. NC_009012.1.

3D structure databases

ProteinModelPortalA3DEU0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203119.Cthe_1237.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN52469; ABN52469; Cthe_1237.
GeneID4809929.
KEGGcth:Cthe_1237.
PATRIC19516249. VBICloThe47081_1305.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMADKPKYYA.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCTHE203119:GIW8-1287-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CLOTH
AccessionPrimary (citable) accession number: A3DEU0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 20, 2007
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries