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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei583ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTHE203119:G1G86-1293-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Cthe_1237
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000093311 – 825Leucine--tRNA ligaseAdd BLAST825

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_1237

Structurei

3D structure databases

ProteinModelPortaliA3DEU0
SMRiA3DEU0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi40 – 50"HIGH" regionAdd BLAST11
Motifi580 – 584"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8T Bacteria
COG0495 LUCA
HOGENOMiHOG000200747
KOiK01869
OMAiTFMVLAP
OrthoDBiPOG091H01JZ

Family and domain databases

Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_00049_B Leu_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR002302 Leu-tRNA-ligase
IPR025709 Leu_tRNA-synth_edit
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR015413 Methionyl/Leucyl_tRNA_Synth
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PANTHERiPTHR43740 PTHR43740, 2 hits
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF13603 tRNA-synt_1_2, 1 hit
PF09334 tRNA-synt_1g, 1 hit
PRINTSiPR00985 TRNASYNTHLEU
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00396 leuS_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A3DEU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYYNFVDIEK KWQKKWLEEK AFAVREDESK KKYYVLEMFP YPSGNLHMGH
60 70 80 90 100
VRNYSIGDVV ARFKRMNGFN VLHPMGWDAF GLPAENAAIK RGVHPNDWTW
110 120 130 140 150
SNIDNMRRQL KQLGISYDWD REVATCHPDY YKWTQWMFLQ LYKNGLAYKK
160 170 180 190 200
KAYVNWCPSC ATVLANEQVV NGVCERCKSV VGKKDLEQWF FKITDYAQRL
210 220 230 240 250
LDDIEKLKGW PDKVKVMQQN WIGRSEGVEV DFKVDGMDKA VRVYTTRPDT
260 270 280 290 300
IYGVTYVVIA PEHPVVKELI KGTEQEQVCN EFINKMMFLN EIDRTATDVE
310 320 330 340 350
KEGVFTGRYV INPLNGDRVP LYLANYVLAE YGTGVVMAVP AHDQRDFEFA
360 370 380 390 400
KKYNLPIKVV IQPEGQELDA SRMTEAFVEV GYLVNSAEFD GVRSDEAIGK
410 420 430 440 450
IIDYIEQKGY GKRKINYRLR DWLISRQRYW GAPIPIIYCD DCGAVPVPEE
460 470 480 490 500
DLPVILPTDI KFSGVGESPL STSETFISAP CPKCGKMGRR ELDTMDTFVC
510 520 530 540 550
SSWYYLRYCD PCNDKAPFDK ERIRYWLPVD QYIGGVEHAI LHLLYSRFLM
560 570 580 590 600
KVLYDLGYVD YDEPFTNLLT QGMVLKDGAK MSKSLGNVVS PEEIIEKYGA
610 620 630 640 650
DTARLFILFA SPPEKDLEWS DQGVEGCYRF INRVWRIVNE FADAVKEGGN
660 670 680 690 700
IDTSTFTKAD KELWYMLNNT LKRVTDDISQ RFNFNTAISA VMELVNSLYY
710 720 730 740 750
YKDKVADDSK NKALVREVIE KLIIMLAPFI PHATEELWSA IGKEGSVHEQ
760 770 780 790 800
KWPSFDPAAL VKDEIEIVVQ INGKVRDKIV VPSDLTKEQV EERALNSEKI
810 820
KAETAGKNVV KVISVPGKLV NIVVK
Length:825
Mass (Da):94,886
Last modified:March 20, 2007 - v1
Checksum:i45416BD31E0D0AEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA Translation: ABN52469.1
RefSeqiWP_003517434.1, NC_009012.1

Genome annotation databases

EnsemblBacteriaiABN52469; ABN52469; Cthe_1237
GeneIDi35802993
KEGGicth:Cthe_1237

Similar proteinsi

Entry informationi

Entry nameiSYL_CLOTH
AccessioniPrimary (citable) accession number: A3DEU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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