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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei583 – 5831ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-1287-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Cthe_1237
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
ProteomesiUP000002145 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 825825Leucine--tRNA ligasePRO_1000009331Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_1237.

Structurei

3D structure databases

ProteinModelPortaliA3DEU0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5011"HIGH" regionAdd
BLAST
Motifi580 – 5845"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3DEU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYYNFVDIEK KWQKKWLEEK AFAVREDESK KKYYVLEMFP YPSGNLHMGH
60 70 80 90 100
VRNYSIGDVV ARFKRMNGFN VLHPMGWDAF GLPAENAAIK RGVHPNDWTW
110 120 130 140 150
SNIDNMRRQL KQLGISYDWD REVATCHPDY YKWTQWMFLQ LYKNGLAYKK
160 170 180 190 200
KAYVNWCPSC ATVLANEQVV NGVCERCKSV VGKKDLEQWF FKITDYAQRL
210 220 230 240 250
LDDIEKLKGW PDKVKVMQQN WIGRSEGVEV DFKVDGMDKA VRVYTTRPDT
260 270 280 290 300
IYGVTYVVIA PEHPVVKELI KGTEQEQVCN EFINKMMFLN EIDRTATDVE
310 320 330 340 350
KEGVFTGRYV INPLNGDRVP LYLANYVLAE YGTGVVMAVP AHDQRDFEFA
360 370 380 390 400
KKYNLPIKVV IQPEGQELDA SRMTEAFVEV GYLVNSAEFD GVRSDEAIGK
410 420 430 440 450
IIDYIEQKGY GKRKINYRLR DWLISRQRYW GAPIPIIYCD DCGAVPVPEE
460 470 480 490 500
DLPVILPTDI KFSGVGESPL STSETFISAP CPKCGKMGRR ELDTMDTFVC
510 520 530 540 550
SSWYYLRYCD PCNDKAPFDK ERIRYWLPVD QYIGGVEHAI LHLLYSRFLM
560 570 580 590 600
KVLYDLGYVD YDEPFTNLLT QGMVLKDGAK MSKSLGNVVS PEEIIEKYGA
610 620 630 640 650
DTARLFILFA SPPEKDLEWS DQGVEGCYRF INRVWRIVNE FADAVKEGGN
660 670 680 690 700
IDTSTFTKAD KELWYMLNNT LKRVTDDISQ RFNFNTAISA VMELVNSLYY
710 720 730 740 750
YKDKVADDSK NKALVREVIE KLIIMLAPFI PHATEELWSA IGKEGSVHEQ
760 770 780 790 800
KWPSFDPAAL VKDEIEIVVQ INGKVRDKIV VPSDLTKEQV EERALNSEKI
810 820
KAETAGKNVV KVISVPGKLV NIVVK
Length:825
Mass (Da):94,886
Last modified:March 20, 2007 - v1
Checksum:i45416BD31E0D0AEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA. Translation: ABN52469.1.
RefSeqiWP_003517434.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN52469; ABN52469; Cthe_1237.
KEGGicth:Cthe_1237.
PATRICi19516249. VBICloThe47081_1305.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA. Translation: ABN52469.1.
RefSeqiWP_003517434.1. NC_009012.1.

3D structure databases

ProteinModelPortaliA3DEU0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203119.Cthe_1237.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN52469; ABN52469; Cthe_1237.
KEGGicth:Cthe_1237.
PATRICi19516249. VBICloThe47081_1305.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-1287-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

Entry informationi

Entry nameiSYL_CLOTH
AccessioniPrimary (citable) accession number: A3DEU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 20, 2007
Last modified: July 22, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.