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Protein

Bifunctional protein FolD

Gene

folD

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei190NADPUniRule annotation1
Binding sitei231NADP; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 167NADPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Oxidoreductase
Biological processAmino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciCTHE203119:G1G86-1140-MONOMER
UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:Cthe_1093
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003058091 – 285Bifunctional protein FolDAdd BLAST285

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi203119.Cthe_1093

Structurei

3D structure databases

ProteinModelPortaliA3DEE6
SMRiA3DEE6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CN0 Bacteria
COG0190 LUCA
HOGENOMiHOG000218242
KOiK01491
OMAiAGKLCGD
OrthoDBiPOG091H0041

Family and domain databases

HAMAPiMF_01576 THF_DHG_CYH, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR000672 THF_DH/CycHdrlase
IPR020630 THF_DH/CycHdrlase_cat_dom
IPR020867 THF_DH/CycHdrlase_CS
IPR020631 THF_DH/CycHdrlase_NAD-bd_dom
PANTHERiPTHR10025 PTHR10025, 1 hit
PfamiView protein in Pfam
PF00763 THF_DHG_CYH, 1 hit
PF02882 THF_DHG_CYH_C, 1 hit
PRINTSiPR00085 THFDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00766 THF_DHG_CYH_1, 1 hit
PS00767 THF_DHG_CYH_2, 1 hit

Sequencei

Sequence statusi: Complete.

A3DEE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAKVLNGKE LSTKIKEELK IEVEDLKKNN INPGLAVIIV GNDPASRVYV
60 70 80 90 100
NSKKKACAEI GIESFEYALE ENTTQEELIE LIHKLNKDDK VSGILVQLPL
110 120 130 140 150
PKHIDEEKVI YAIDPSKDVD AFHPVNVGKL MIGNPDFLPC TPAGVMELIK
160 170 180 190 200
ESGIDITGKE CVVVGRSNIV GKPMAMLLLA QNGTVTVCHS RTQNIDEVCK
210 220 230 240 250
RADILVVAVG KPEFIKGSSI KPGAVVIDVG INRLENKKLV GDVEYESASR
260 270 280
VASAITPVPG GVGPMTIAML MKNTVKAAKL QAKMK
Length:285
Mass (Da):30,787
Last modified:March 20, 2007 - v1
Checksum:i9753F4F06A1B1B83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA Translation: ABN52325.1
RefSeqiWP_003515736.1, NC_009012.1

Genome annotation databases

EnsemblBacteriaiABN52325; ABN52325; Cthe_1093
GeneIDi35804655
KEGGicth:Cthe_1093

Similar proteinsi

Entry informationi

Entry nameiFOLD_CLOTH
AccessioniPrimary (citable) accession number: A3DEE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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