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Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase

Gene

panB

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)
  2. no protein annotated in this organism
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi46MagnesiumUniRule annotation1
Metal bindingi85MagnesiumUniRule annotation1
Binding sitei85Alpha-ketoisovalerateUniRule annotation1
Binding sitei115Alpha-ketoisovalerateUniRule annotation1
Metal bindingi117MagnesiumUniRule annotation1
Active sitei184Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPantothenate biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciCTHE203119:G1G86-940-MONOMER
UniPathwayiUPA00028; UER00003

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferaseUniRule annotation (EC:2.1.2.11UniRule annotation)
Alternative name(s):
Ketopantoate hydroxymethyltransferaseUniRule annotation
Short name:
KPHMTUniRule annotation
Gene namesi
Name:panBUniRule annotation
Ordered Locus Names:Cthe_0902
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002972481 – 2833-methyl-2-oxobutanoate hydroxymethyltransferaseAdd BLAST283

Interactioni

Subunit structurei

Homodecamer; pentamer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi203119.Cthe_0902

Structurei

3D structure databases

ProteinModelPortaliA3DDV7
SMRiA3DDV7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni46 – 47Alpha-ketoisovalerate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PanB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCG Bacteria
COG0413 LUCA
HOGENOMiHOG000078427
KOiK00606
OMAiTEDMIGA
OrthoDBiPOG091H02K8

Family and domain databases

CDDicd06557 KPHMT-like, 1 hit
HAMAPiMF_00156 PanB, 1 hit
InterProiView protein in InterPro
IPR003700 Pantoate_hydroxy_MeTrfase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERiPTHR20881 PTHR20881, 1 hit
PfamiView protein in Pfam
PF02548 Pantoate_transf, 1 hit
PIRSFiPIRSF000388 Pantoate_hydroxy_MeTrfase, 1 hit
SUPFAMiSSF51621 SSF51621, 1 hit
TIGRFAMsiTIGR00222 panB, 1 hit

Sequencei

Sequence statusi: Complete.

A3DDV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDKFTTASF LESKKQGRKI TMLTAYDYPT AKILDEAGVD SILVGDSLGM
60 70 80 90 100
VVLGYEDTTR VTMDDMVHHT KAVVRGVKRA MVVADMPFLS YHTGKNDSVR
110 120 130 140 150
NAGRLVSEGG CKAVKLEGGE DIIENVKAII SAGIPVVGHL GYTPQSINVF
160 170 180 190 200
GGHKAQGKTI DTAKKIYRDA LLLQKAGVFA VVLECVPYKL AAFISKRLDI
210 220 230 240 250
PTIGIGSGPD CDGQVLVIHD LTGMFKDFTP KHVKKYAEIG ETVSTAVKSY
260 270 280
IDEVKNGVFP TEKNSFKVDD WIIDELDKVR FDI
Length:283
Mass (Da):30,876
Last modified:March 20, 2007 - v1
Checksum:i4A6CDC9D581834E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA Translation: ABN52136.1
RefSeqiWP_003517259.1, NC_009012.1

Genome annotation databases

EnsemblBacteriaiABN52136; ABN52136; Cthe_0902
GeneIDi35802922
KEGGicth:Cthe_0902

Similar proteinsi

Entry informationi

Entry nameiPANB_CLOTH
AccessioniPrimary (citable) accession number: A3DDV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health