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A3DDN1

- GUND_CLOTH

UniProt

A3DDN1 - GUND_CLOTH

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Protein
Endoglucanase D
Gene
celD, Cthe_0825
Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans By similarity.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Cofactori

Calcium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei201 – 2011Nucleophile By similarity
Active sitei516 – 5161 By similarity
Active sitei546 – 5461 By similarity
Active sitei555 – 5551Proton donor By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-854-MONOMER.
MetaCyc:MONOMER-16416.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase D (EC:3.2.1.4)
Short name:
EGD
Alternative name(s):
Cellulase D
Endo-1,4-beta-glucanase
Gene namesi
Name:celD
Ordered Locus Names:Cthe_0825
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
ProteomesiUP000002145: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4141 By similarity
Add
BLAST
Chaini42 – 649608Endoglucanase D
PRO_0000284722Add
BLAST

Proteomic databases

PRIDEiA3DDN1.

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0825.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi594 – 60411
Helixi614 – 6207
Helixi630 – 64011
Beta strandi643 – 6453

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FL4X-ray2.90A/D/G/J584-649[»]
ProteinModelPortaliA3DDN1.
SMRiA3DDN1. Positions 35-575, 580-649.

Miscellaneous databases

EvolutionaryTraceiA3DDN1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati585 – 608241
Add
BLAST
Repeati621 – 644242
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni585 – 644602 X 24 AA approximate repeats
Add
BLAST

Domaini

A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component By similarity.

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG05134.
HOGENOMiHOG000245359.
KOiK01179.
OMAiQQDAKEC.
OrthoDBiEOG6BCSN2.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR004197. Glyco_hydro_9_Ig-like.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02927. CelD_N. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF63446. SSF63446. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 2 hits.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A3DDN1-1 [UniParc]FASTAAdd to Basket

« Hide

MSRMTLKSSM KKRVLSLLIA VVFLSLTGVF PSGLIETKVS AAKITENYQF    50
DSRIRLNSIG FIPNHSKKAT IAANCSTFYV VKEDGTIVYT GTATSMFDND 100
TKETVYIADF SSVNEEGTYY LAVPGVGKSV NFKIAMNVYE DAFKTAMLGM 150
YLLRCGTSVS ATYNGIHYSH GPCHTNDAYL DYINGQHTKK DSTKGWHDAG 200
DYNKYVVNAG ITVGSMFLAW EHFKDQLEPV ALEIPEKNNS IPDFLDELKY 250
EIDWILTMQY PDGSGRVAHK VSTRNFGGFI MPENEHDERF FVPWSSAATA 300
DFVAMTAMAA RIFRPYDPQY AEKCINAAKV SYEFLKNNPA NVFANQSGFS 350
TGEYATVSDA DDRLWAAAEM WETLGDEEYL RDFENRAAQF SKKIEADFDW 400
DNVANLGMFT YLLSERPGKN PALVQSIKDS LLSTADSIVR TSQNHGYGRT 450
LGTTYYWGCN GTVVRQTMIL QVANKISPNN DYVNAALDAI SHVFGRNYYN 500
RSYVTGLGIN PPMNPHDRRS GADGIWEPWP GYLVGGGWPG PKDWVDIQDS 550
YQTNEIAINW NAALIYALAG FVNYNSAQNE VLYGDVNDDG KVNSTDLTLL 600
KRYVLKAVST LPSSKAEKNA DVNRDGRVNS SDVTILSRYL IRVIEKLPI 649
Length:649
Mass (Da):72,415
Last modified:March 20, 2007 - v1
Checksum:i3A6A44735B29D3F4
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti577 – 5771A → P in CAA28255. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04584 Genomic DNA. Translation: CAA28255.1.
CP000568 Genomic DNA. Translation: ABN52060.1.
PIRiA25535. CZCLDM.
RefSeqiYP_001037253.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN52060; ABN52060; Cthe_0825.
GeneIDi4810443.
KEGGicth:Cthe_0825.
PATRICi19515357. VBICloThe47081_0867.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04584 Genomic DNA. Translation: CAA28255.1 .
CP000568 Genomic DNA. Translation: ABN52060.1 .
PIRi A25535. CZCLDM.
RefSeqi YP_001037253.1. NC_009012.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4FL4 X-ray 2.90 A/D/G/J 584-649 [» ]
ProteinModelPortali A3DDN1.
SMRi A3DDN1. Positions 35-575, 580-649.
ModBasei Search...

Protein-protein interaction databases

STRINGi 203119.Cthe_0825.

Protein family/group databases

CAZyi GH9. Glycoside Hydrolase Family 9.

Proteomic databases

PRIDEi A3DDN1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN52060 ; ABN52060 ; Cthe_0825 .
GeneIDi 4810443.
KEGGi cth:Cthe_0825.
PATRICi 19515357. VBICloThe47081_0867.

Phylogenomic databases

eggNOGi NOG05134.
HOGENOMi HOG000245359.
KOi K01179.
OMAi QQDAKEC.
OrthoDBi EOG6BCSN2.

Enzyme and pathway databases

BioCyci CTHE203119:GIW8-854-MONOMER.
MetaCyc:MONOMER-16416.

Miscellaneous databases

EvolutionaryTracei A3DDN1.

Family and domain databases

Gene3Di 1.10.1330.10. 1 hit.
1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR004197. Glyco_hydro_9_Ig-like.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
Pfami PF02927. CelD_N. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
SSF63446. SSF63446. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 2 hits.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the cellulase gene celD encoding endoglucanase D of Clostridium thermocellum."
    Joliff G., Beguin P., Aubert J.-P.
    Nucleic Acids Res. 14:8605-8613(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27405 / DSM 1237.

Entry informationi

Entry nameiGUND_CLOTH
AccessioniPrimary (citable) accession number: A3DDN1
Secondary accession number(s): P04954
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: March 20, 2007
Last modified: September 3, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi