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Protein

Endoglucanase D

Gene

celD

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.By similarity

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei201NucleophilePROSITE-ProRule annotation1
Active sitei516By similarity1
Active sitei546By similarity1
Active sitei555Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation
LigandCalcium

Enzyme and pathway databases

BioCyciCTHE203119:G1G86-860-MONOMER
MetaCyc:MONOMER-16416

Protein family/group databases

CAZyiGH9 Glycoside Hydrolase Family 9

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase D (EC:3.2.1.4)
Short name:
EGD
Alternative name(s):
Cellulase D
Endo-1,4-beta-glucanase
Gene namesi
Name:celD
Ordered Locus Names:Cthe_0825
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 41By similarityAdd BLAST41
ChainiPRO_000028472242 – 649Endoglucanase DAdd BLAST608

Proteomic databases

PRIDEiA3DDN1

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0825

Structurei

Secondary structure

1649
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi594 – 604Combined sources11
Helixi614 – 620Combined sources7
Helixi630 – 640Combined sources11
Beta strandi643 – 645Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4FL4X-ray2.90A/D/G/J584-649[»]
ProteinModelPortaliA3DDN1
SMRiA3DDN1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA3DDN1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini579 – 649DockerinPROSITE-ProRule annotationAdd BLAST71

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105E08 Bacteria
ENOG410XNTA LUCA
HOGENOMiHOG000245359
KOiK01179
OMAiYYTQRSG
OrthoDBiPOG091H04TS

Family and domain databases

CDDicd02850 E_set_Cellulase_N, 1 hit
Gene3Di1.50.10.10, 1 hit
2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR004197 Cellulase_Ig-like
IPR002105 Dockerin_1_rpt
IPR016134 Dockerin_dom
IPR036439 Dockerin_dom_sf
IPR001701 Glyco_hydro_9
IPR033126 Glyco_hydro_9_Asp/Glu_AS
IPR018221 Glyco_hydro_9_His_AS
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PfamiView protein in Pfam
PF02927 CelD_N, 1 hit
PF00404 Dockerin_1, 2 hits
PF00759 Glyco_hydro_9, 1 hit
SUPFAMiSSF48208 SSF48208, 1 hit
SSF63446 SSF63446, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00448 CLOS_CELLULOSOME_RPT, 2 hits
PS51766 DOCKERIN, 1 hit
PS00018 EF_HAND_1, 2 hits
PS00592 GLYCOSYL_HYDROL_F9_1, 1 hit
PS00698 GLYCOSYL_HYDROL_F9_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A3DDN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRMTLKSSM KKRVLSLLIA VVFLSLTGVF PSGLIETKVS AAKITENYQF
60 70 80 90 100
DSRIRLNSIG FIPNHSKKAT IAANCSTFYV VKEDGTIVYT GTATSMFDND
110 120 130 140 150
TKETVYIADF SSVNEEGTYY LAVPGVGKSV NFKIAMNVYE DAFKTAMLGM
160 170 180 190 200
YLLRCGTSVS ATYNGIHYSH GPCHTNDAYL DYINGQHTKK DSTKGWHDAG
210 220 230 240 250
DYNKYVVNAG ITVGSMFLAW EHFKDQLEPV ALEIPEKNNS IPDFLDELKY
260 270 280 290 300
EIDWILTMQY PDGSGRVAHK VSTRNFGGFI MPENEHDERF FVPWSSAATA
310 320 330 340 350
DFVAMTAMAA RIFRPYDPQY AEKCINAAKV SYEFLKNNPA NVFANQSGFS
360 370 380 390 400
TGEYATVSDA DDRLWAAAEM WETLGDEEYL RDFENRAAQF SKKIEADFDW
410 420 430 440 450
DNVANLGMFT YLLSERPGKN PALVQSIKDS LLSTADSIVR TSQNHGYGRT
460 470 480 490 500
LGTTYYWGCN GTVVRQTMIL QVANKISPNN DYVNAALDAI SHVFGRNYYN
510 520 530 540 550
RSYVTGLGIN PPMNPHDRRS GADGIWEPWP GYLVGGGWPG PKDWVDIQDS
560 570 580 590 600
YQTNEIAINW NAALIYALAG FVNYNSAQNE VLYGDVNDDG KVNSTDLTLL
610 620 630 640
KRYVLKAVST LPSSKAEKNA DVNRDGRVNS SDVTILSRYL IRVIEKLPI
Length:649
Mass (Da):72,415
Last modified:March 20, 2007 - v1
Checksum:i3A6A44735B29D3F4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti577A → P in CAA28255 (PubMed:3024110).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04584 Genomic DNA Translation: CAA28255.1
CP000568 Genomic DNA Translation: ABN52060.1
PIRiA25535 CZCLDM
RefSeqiWP_011837914.1, NC_009012.1

Genome annotation databases

EnsemblBacteriaiABN52060; ABN52060; Cthe_0825
GeneIDi35803078
KEGGicth:Cthe_0825

Similar proteinsi

Entry informationi

Entry nameiGUND_CLOTH
AccessioniPrimary (citable) accession number: A3DDN1
Secondary accession number(s): P04954
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health