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Protein

NAD kinase

Gene

nadK

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei68Proton acceptorUniRule annotation1
Binding sitei73NADUniRule annotation1
Binding sitei153NADUniRule annotation1
Binding sitei172NADUniRule annotation1
Binding sitei243NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi68 – 69NADUniRule annotation2
Nucleotide bindingi142 – 143NADUniRule annotation2
Nucleotide bindingi183 – 188NADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.6.1.83. 1530.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:Cthe_0816
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000794851 – 289NAD kinaseAdd BLAST289

Proteomic databases

PRIDEiA3DDM2.

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0816.

Structurei

3D structure databases

ProteinModelPortaliA3DDM2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F91. Bacteria.
COG0061. LUCA.
HOGENOMiHOG000227222.
KOiK00858.
OMAiKLHWHEY.
OrthoDBiPOG091H02D1.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

A3DDM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKIGIIPNE DKDEELKYTR ILVDSIKKCG GTAIVCDDIA LKLGDKESNI
60 70 80 90 100
NEDNIVDMSD VMVCLGGDGT FLKAARMTVV KGKPLLGVNL GKLGFLADVD
110 120 130 140 150
KNDIENAVKR LVEDKFTVDE RMMLDTVIVR DGKIIAEDIV LNDVVISRGA
160 170 180 190 200
ISRILHLKTY INDAFMDLYP GDGLIISTPT GSTAYSLSAG GPLVEPDVDL
210 220 230 240 250
IICTPICPHL LYSRSFITTA DRVIKVVVAE SSSHEAMVTV DGQNGYEVRG
260 270 280
GDVIITKKSR IRMPMVRLNG KNFFDVLRGK IYDRGESMK
Length:289
Mass (Da):31,670
Last modified:March 20, 2007 - v1
Checksum:iAF95C884EB114B6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA. Translation: ABN52051.1.
RefSeqiWP_003518725.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN52051; ABN52051; Cthe_0816.
KEGGicth:Cthe_0816.
PATRICi19515339. VBICloThe47081_0858.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA. Translation: ABN52051.1.
RefSeqiWP_003518725.1. NC_009012.1.

3D structure databases

ProteinModelPortaliA3DDM2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203119.Cthe_0816.

Proteomic databases

PRIDEiA3DDM2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN52051; ABN52051; Cthe_0816.
KEGGicth:Cthe_0816.
PATRICi19515339. VBICloThe47081_0858.

Phylogenomic databases

eggNOGiENOG4105F91. Bacteria.
COG0061. LUCA.
HOGENOMiHOG000227222.
KOiK00858.
OMAiKLHWHEY.
OrthoDBiPOG091H02D1.

Enzyme and pathway databases

BRENDAi2.6.1.83. 1530.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADK_CLOTH
AccessioniPrimary (citable) accession number: A3DDM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 20, 2007
Last modified: November 2, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.