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Protein

Ribonuclease H

Gene

rnhA

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9Magnesium 1UniRule annotation1
Metal bindingi9Magnesium 2UniRule annotation1
Metal bindingi47Magnesium 1UniRule annotation1
Metal bindingi69Magnesium 1UniRule annotation1
Metal bindingi134Magnesium 2UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciCTHE203119:G1G86-706-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HUniRule annotation (EC:3.1.26.4UniRule annotation)
Short name:
RNase HUniRule annotation
Gene namesi
Name:rnhAUniRule annotation
Ordered Locus Names:Cthe_0673
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003325831 – 147Ribonuclease HAdd BLAST147

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi203119.Cthe_0673

Structurei

3D structure databases

ProteinModelPortaliA3DD79
SMRiA3DD79
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 142RNase HUniRule annotationAdd BLAST142

Sequence similaritiesi

Belongs to the RNase H family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UMW Bacteria
COG0328 LUCA
HOGENOMiHOG000040465
KOiK03469
OMAiMQEIEIF
OrthoDBiPOG091H04XT

Family and domain databases

CDDicd09278 RNase_HI_prokaryote_like, 1 hit
Gene3Di3.30.420.10, 1 hit
HAMAPiMF_00042 RNase_H, 1 hit
InterProiView protein in InterPro
IPR017290 RNase_H_bac
IPR012337 RNaseH-like_sf
IPR002156 RNaseH_domain
IPR036397 RNaseH_sf
IPR022892 RNaseHI
PfamiView protein in Pfam
PF00075 RNase_H, 1 hit
PIRSFiPIRSF037839 Ribonuclease_H, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
PROSITEiView protein in PROSITE
PS50879 RNASE_H, 1 hit

Sequencei

Sequence statusi: Complete.

A3DD79-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVSIYTDG ACSGNPGDGG WGAILIYGNH EKEVSGFEKD TTNNRMELVA
60 70 80 90 100
AINALKMLKE PCEVDLYSDS AYLVNGFLQN WVEKWKKNGW KTSNKEEVKN
110 120 130 140
MELWQELDRL SNIHKIRWIK VKGHSDNEYN NRCDKLATDE IKKNSKK
Length:147
Mass (Da):16,913
Last modified:March 20, 2007 - v1
Checksum:i441625A11CC0CFAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA Translation: ABN51908.1
RefSeqiWP_003516659.1, NC_009012.1

Genome annotation databases

EnsemblBacteriaiABN51908; ABN51908; Cthe_0673
GeneIDi35804764
KEGGicth:Cthe_0673

Similar proteinsi

Entry informationi

Entry nameiRNH_CLOTH
AccessioniPrimary (citable) accession number: A3DD79
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health