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Protein

Thiazole synthase

Gene

thiG

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei97Schiff-base intermediate with DXPUniRule annotation1
Binding sitei158DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

BioCyciCTHE203119:G1G86-625-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:Cthe_0598
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000260031 – 255Thiazole synthaseAdd BLAST255

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi203119.Cthe_0598

Structurei

3D structure databases

ProteinModelPortaliA3DD04
SMRiA3DD04
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 185DXP bindingUniRule annotation2
Regioni206 – 207DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8 Bacteria
COG2022 LUCA
HOGENOMiHOG000248049
KOiK03149
OMAiAQYPSPA
OrthoDBiPOG091H01K9

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

A3DD04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDKLIIGGR EIISRLFIGT GKFPANRIIP DVVRMSGAQV VTVALRRIDF
60 70 80 90 100
DSDEENMLRY IPKDCILMPN TSGARNAEEA VRIARLARAS GCGNFIKIEV
110 120 130 140 150
ISDNRYLLPD NLETIKATEI LVKEGFIVLP YMNPDLMDAR RLKDAGAAAV
160 170 180 190 200
MPLGAPIGTN KGLKTREMIK ILIDEIDIPV IVDAGIGKPS DACEAMELGA
210 220 230 240 250
DAVLVNTAIA TAGDPVVMAE AFANAVIAGR KAYLSGLGPV KEYAEASSPL

TGFLR
Length:255
Mass (Da):27,371
Last modified:March 20, 2007 - v1
Checksum:iD34AA48D5DC7BC82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA Translation: ABN51833.1
RefSeqiWP_003516495.1, NC_009012.1

Genome annotation databases

EnsemblBacteriaiABN51833; ABN51833; Cthe_0598
GeneIDi35803384
KEGGicth:Cthe_0598

Similar proteinsi

Entry informationi

Entry nameiTHIG_CLOTH
AccessioniPrimary (citable) accession number: A3DD04
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health