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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi486MagnesiumUniRule annotation1
Metal bindingi492MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciCTHE203119:G1G86-437-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Cthe_0418
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003296051 – 700Polyribonucleotide nucleotidyltransferaseAdd BLAST700

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0418

Structurei

3D structure databases

ProteinModelPortaliA3DCH7
SMRiA3DCH7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini554 – 613KHUniRule annotationAdd BLAST60
Domaini623 – 691S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62 Bacteria
COG1185 LUCA
HOGENOMiHOG000218327
KOiK00962
OMAiRYMHNYN
OrthoDBiPOG091H00M0

Family and domain databases

Gene3Di3.30.1370.10, 1 hit
3.30.230.70, 2 hits
HAMAPiMF_01595 PNPase, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
IPR012340 NA-bd_OB-fold
IPR012162 PNPase
IPR027408 PNPase/RNase_PH_dom_sf
IPR015848 PNPase_PH_RNA-bd_bac/org-type
IPR036456 PNPase_PH_RNA-bd_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR022967 S1_dom
IPR003029 S1_domain
PANTHERiPTHR11252 PTHR11252, 1 hit
PfamiView protein in Pfam
PF00013 KH_1, 1 hit
PF03726 PNPase, 1 hit
PF01138 RNase_PH, 2 hits
PF03725 RNase_PH_C, 1 hit
PF00575 S1, 1 hit
PIRSFiPIRSF005499 PNPase, 1 hit
SMARTiView protein in SMART
SM00322 KH, 1 hit
SM00316 S1, 1 hit
SUPFAMiSSF46915 SSF46915, 1 hit
SSF50249 SSF50249, 1 hit
SSF54211 SSF54211, 2 hits
SSF54791 SSF54791, 1 hit
SSF55666 SSF55666, 2 hits
TIGRFAMsiTIGR03591 polynuc_phos, 1 hit
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 1 hit
PS50126 S1, 1 hit

Sequencei

Sequence statusi: Complete.

A3DCH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKTFSMELA GRTLTIETGK LAQLANGSVL VRYGDTVVLS TATASATPRE
60 70 80 90 100
GVDFFPLSVD YEERLYAVGK IPGGFIKREG KPSEKAILTA RVIDRPLRPL
110 120 130 140 150
FPKDLRNDVA IVNTVLSVDQ DNSPELAALL GSSIAVSISD IPFNGPVGAV
160 170 180 190 200
ILGLIDGEVI INPTEKQKEI SQMYVTLAGT RNKIVMIEAG ANEVPDEVML
210 220 230 240 250
DAIKKGHEEI KKIVDFIDGI VKEVGKPKFE YESAEVPEEI FNAVREYAYD
260 270 280 290 300
KMREAVLAVD KQVRDKNIDD LTKEITEHFA EVFPEMEPAI KEAIYKLEKK
310 320 330 340 350
VVREYILEEG RRVDGRRLDE IRPLSAEVGL LPRVHGSGLF TRGQTQVLSS
360 370 380 390 400
VTLGAMGDVQ ILDGIDTEET KRYMHHYNFP GFSVGEAKSS RGPGRREIGH
410 420 430 440 450
GALAERALEP VIPSEEEFPY TIRVVSEVLM SNGSTSQGSV CGSTLALMDA
460 470 480 490 500
GVPIKKPVAG ISAGLVVDEN NPDRFVTFMD IQGIEDFFGD MDFKVAGTKD
510 520 530 540 550
GITAIQVDIK IDGLTEEIIK QAFELTRKGR LYIIDNVLLK AIPEPRKQMS
560 570 580 590 600
KYAPKIISTT INPDKIREVI GPGGKMINKI IDETGVKIDI NDDGRVYIFS
610 620 630 640 650
SDIQAGKRAR SMIEAIAKDI EPGQVFLGRV IRVTSFGAFV EFLPGKEGLV
660 670 680 690 700
HISKLDKKRV ERVEDIVRVG DQILVKVIEI DKQGRVNLSR KDAMEDEWDK
Length:700
Mass (Da):77,318
Last modified:March 20, 2007 - v1
Checksum:i4DAC5DD2A1704F59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA Translation: ABN51656.1
RefSeqiWP_004463117.1, NC_009012.1

Genome annotation databases

EnsemblBacteriaiABN51656; ABN51656; Cthe_0418
GeneIDi35804284
KEGGicth:Cthe_0418

Similar proteinsi

Entry informationi

Entry nameiPNP_CLOTH
AccessioniPrimary (citable) accession number: A3DCH7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health